Optimization of cytotoxic assay by target cell retention of the fluorescent dye carboxyfluorescein diacetate (CFDA) and comparison with conventional 51CR release assay

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Abstract

This paper describes the comparison between a fluorimetric NK assay based on the target cell retention of fluorescent dye carboxyfluorescein diacetate (cFDA) and standard 51Cr release assay. The results provide several suggestions to improve the cytotoxic assay based on the use of the fluorogenic substrate showing that the measurements of cFDA retained by target cells represent a method of evaluating cytotoxicity completely comparable to the 51Cr release assay.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalJournal of Immunological Methods
Volume155
Issue number1
DOIs
Publication statusPublished - Oct 19 1992

Keywords

  • Carboxyfluorescein diacetate
  • Cytotoxic assay
  • Fluorimetric analysis

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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