Optimization of the bone regeneration capacity of gelatin-based cryogels via electromagnetic stimulation

Lorenzo Fassina, Enrica Saino, Sandra Van Vlierberghe, Jorg Schelfhout, Manuel Dierick, Luc Van Hoorebeke, Peter Dubruel, Francesco Benazzo, Livia Visai, Giovanni Magenes

Research output: Chapter in Book/Report/Conference proceedingChapter


One of the key challenges in reconstructive bone surgery is to provide living constructs that possess the ability to integrate in the surrounding host tissue. Bone graft substitutes and biomaterials have already been widely used to heal critical-size bone defects due to trauma, tumor resection and tissue degeneration. In the present study, gelatin-based cryogels have been seeded with human SAOS-2 osteoblasts followed by the in vitro culture of the cells. In order to overcome the drawbacks associated with static culture systems, including limited diffusion and inhomogeneous cell-matrix distribution, the present work describes the application of a bioreactor to physically enhance the cell culture in vitro using an electromagnetic stimulus. The results indicate that the physical stimulation of cell-seeded gelatin-based cryogels upregulates the bone matrix production. We anticipate that the scaffolds developed consisting of human bone proteins and cells could be applied for clinical purposes related to bone repair.

Original languageEnglish
Title of host publicationBone Grafts: Procedures, Complications and Alternatives
PublisherNova Science Publishers, Inc.
Number of pages12
ISBN (Print)9781622576999
Publication statusPublished - Feb 2013


  • Biomimetics
  • Bone tissue engineering
  • Cryogel
  • Electromagnetic stimulation
  • Gelatin

ASJC Scopus subject areas

  • Medicine(all)


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