Optimization of the bone regeneration capacity of gelatin-based cryogels via electromagnetic stimulation

Lorenzo Fassina, Enrica Saino, Sandra Van Vlierberghe, Jorg Schelfhout, Manuel Dierick, Luc Van Hoorebeke, Peter Dubruel, Francesco Benazzo, Livia Visai, Giovanni Magenes

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

One of the key challenges in reconstructive bone surgery is to provide living constructs that possess the ability to integrate in the surrounding host tissue. Bone graft substitutes and biomaterials have already been widely used to heal critical-size bone defects due to trauma, tumor resection and tissue degeneration. In the present study, gelatin-based cryogels have been seeded with human SAOS-2 osteoblasts followed by the in vitro culture of the cells. In order to overcome the drawbacks associated with static culture systems, including limited diffusion and inhomogeneous cell-matrix distribution, the present work describes the application of a bioreactor to physically enhance the cell culture in vitro using an electromagnetic stimulus. The results indicate that the physical stimulation of cell-seeded gelatin-based cryogels upregulates the bone matrix production. We anticipate that the scaffolds developed consisting of human bone proteins and cells could be applied for clinical purposes related to bone repair.

Original languageEnglish
Title of host publicationBone Grafts: Procedures, Complications and Alternatives
PublisherNova Science Publishers, Inc.
Pages1-12
Number of pages12
ISBN (Print)9781622576999
Publication statusPublished - Feb 2013

Fingerprint

Cryogels
Bone Regeneration
Electromagnetic Phenomena
Gelatin
Bone and Bones
Reconstructive Surgical Procedures
Physical Stimulation
Cell Culture Techniques
Bone Substitutes
Bone Matrix
Biocompatible Materials
Bioreactors
Osteoblasts
Up-Regulation
Transplants
Wounds and Injuries
Neoplasms
Proteins

Keywords

  • Biomimetics
  • Bone tissue engineering
  • Cryogel
  • Electromagnetic stimulation
  • Gelatin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Fassina, L., Saino, E., Van Vlierberghe, S., Schelfhout, J., Dierick, M., Van Hoorebeke, L., ... Magenes, G. (2013). Optimization of the bone regeneration capacity of gelatin-based cryogels via electromagnetic stimulation. In Bone Grafts: Procedures, Complications and Alternatives (pp. 1-12). Nova Science Publishers, Inc..

Optimization of the bone regeneration capacity of gelatin-based cryogels via electromagnetic stimulation. / Fassina, Lorenzo; Saino, Enrica; Van Vlierberghe, Sandra; Schelfhout, Jorg; Dierick, Manuel; Van Hoorebeke, Luc; Dubruel, Peter; Benazzo, Francesco; Visai, Livia; Magenes, Giovanni.

Bone Grafts: Procedures, Complications and Alternatives. Nova Science Publishers, Inc., 2013. p. 1-12.

Research output: Chapter in Book/Report/Conference proceedingChapter

Fassina, L, Saino, E, Van Vlierberghe, S, Schelfhout, J, Dierick, M, Van Hoorebeke, L, Dubruel, P, Benazzo, F, Visai, L & Magenes, G 2013, Optimization of the bone regeneration capacity of gelatin-based cryogels via electromagnetic stimulation. in Bone Grafts: Procedures, Complications and Alternatives. Nova Science Publishers, Inc., pp. 1-12.
Fassina L, Saino E, Van Vlierberghe S, Schelfhout J, Dierick M, Van Hoorebeke L et al. Optimization of the bone regeneration capacity of gelatin-based cryogels via electromagnetic stimulation. In Bone Grafts: Procedures, Complications and Alternatives. Nova Science Publishers, Inc. 2013. p. 1-12
Fassina, Lorenzo ; Saino, Enrica ; Van Vlierberghe, Sandra ; Schelfhout, Jorg ; Dierick, Manuel ; Van Hoorebeke, Luc ; Dubruel, Peter ; Benazzo, Francesco ; Visai, Livia ; Magenes, Giovanni. / Optimization of the bone regeneration capacity of gelatin-based cryogels via electromagnetic stimulation. Bone Grafts: Procedures, Complications and Alternatives. Nova Science Publishers, Inc., 2013. pp. 1-12
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