Optimization of the isolation and expansion method of human mediastinal–adipose tissue derived mesenchymal stem cells with virally inactivated GMP-grade platelet lysate

Camilla Siciliano, Mohsen Ibrahim, Gaia Scafetta, Chiara Napoletano, Giorgio Mangino, Luca Pierelli, Giacomo Frati, Elena De Falco

Research output: Contribution to journalArticle

Abstract

Mesenchymal stem cells (MSCs) are adult multipotent cells currently employed in several clinical trials due to their immunomodulating, angiogenic and repairing features. The adipose tissue is certainly considered an eligible source of MSCs. Recently, putative adipose tissue derived MSCs (ADMSCs) have been isolated from the mediastinal depots. However, very little is known about the properties, the function and the potential of human mediastinal ADMSCs (hmADMSCs). However, the lack of standardized methodologies to culture ADMSCs prevents comparison across. Herein for the first time, we report a detailed step by step description to optimize the isolation and the expansion methodology of hmADMSCs using a virally inactivated good manufacturing practice (GMP)-grade platelet lysate, highlighting the critical aspects of the procedure and providing useful troubleshooting suggestions. Our approach offers a reproducible system which could provide standardization across laboratories. Moreover, our system is time and cost effective, and it can provide a reproducible source of adipose stem cells to enable future studies to unravel new insights regard this promising stem cell population.

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalCytotechnology
Volume67
Issue number1
DOIs
Publication statusPublished - 2013

Keywords

  • Adipose tissue
  • Good manufacturing practices
  • Mesenchymal stem cells
  • Platelet lysate

ASJC Scopus subject areas

  • Cell Biology
  • Biotechnology
  • Biomedical Engineering
  • Bioengineering
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Optimization of the isolation and expansion method of human mediastinal–adipose tissue derived mesenchymal stem cells with virally inactivated GMP-grade platelet lysate'. Together they form a unique fingerprint.

  • Cite this