### Abstract

We analyzed the results of 71 leukapheresis procedures performed in 21 patients to identify the best predictive factors affecting the yield of peripheral blood progenitors after high-dose chemotherapy followed by G-CSF administration. An average of 1 ± 1 x 10^{8} MNC/kg, 5 ± 6 x 10^{4} CFU-GM/kg and 4 ± 6 x 10^{6} CD34+ cells/kg was collected for each leukapheresis. When we defined ≥ 5 x 10^{4}/kg as the minimum number of CFU-GM per procedure for a 'satisfactory' collection, multiparameter analysis of clinical features and laboratory findings showed that the only factors that predicted the numbers of CFU-GM collected were prior treatment with the MOPP regimen and the number of mononuclear cells identified in the basophil channel of the H*1=Technicon. A logistic regression analysis performed to generate a mathematical model revealed four predictive factors: the number of previous cycles of chemotherapy, previous MOPP chemotherapy, the interval from latest chemotherapy and the number of mononuclear cells/μl. This model was valuable in defining the optimal time for the first leukapheresis procedure. In contrast, the number of circulating CD34+ cells did not correlate with CFU-GM numbers collected whereas the numbers of mononuclear cells did provide a simple and reliable index. Thus the principal factor affecting the efficiency of peripheral blood stem cell collection was prior therapy with MOPP.

Original language | English |
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Pages (from-to) | 273-278 |

Number of pages | 6 |

Journal | Bone Marrow Transplantation |

Volume | 14 |

Issue number | 2 |

Publication status | Published - 1994 |

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### ASJC Scopus subject areas

- Hematology
- Transplantation

### Cite this

*Bone Marrow Transplantation*,

*14*(2), 273-278.