Optimization of the yield of PBSC for autotransplantation mobilized by high-dose chemotherapy and G-CSF: Proposal for a mathematical model

A. Olivieri, M. Offidani, L. Ciniero, A. Poloni, M. C. Masia, A. Salvi, P. Leoni

Research output: Contribution to journalArticle

Abstract

We analyzed the results of 71 leukapheresis procedures performed in 21 patients to identify the best predictive factors affecting the yield of peripheral blood progenitors after high-dose chemotherapy followed by G-CSF administration. An average of 1 ± 1 x 108 MNC/kg, 5 ± 6 x 104 CFU-GM/kg and 4 ± 6 x 106 CD34+ cells/kg was collected for each leukapheresis. When we defined ≥ 5 x 104/kg as the minimum number of CFU-GM per procedure for a 'satisfactory' collection, multiparameter analysis of clinical features and laboratory findings showed that the only factors that predicted the numbers of CFU-GM collected were prior treatment with the MOPP regimen and the number of mononuclear cells identified in the basophil channel of the H*1=Technicon. A logistic regression analysis performed to generate a mathematical model revealed four predictive factors: the number of previous cycles of chemotherapy, previous MOPP chemotherapy, the interval from latest chemotherapy and the number of mononuclear cells/μl. This model was valuable in defining the optimal time for the first leukapheresis procedure. In contrast, the number of circulating CD34+ cells did not correlate with CFU-GM numbers collected whereas the numbers of mononuclear cells did provide a simple and reliable index. Thus the principal factor affecting the efficiency of peripheral blood stem cell collection was prior therapy with MOPP.

Original languageEnglish
Pages (from-to)273-278
Number of pages6
JournalBone Marrow Transplantation
Volume14
Issue number2
Publication statusPublished - 1994

    Fingerprint

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Olivieri, A., Offidani, M., Ciniero, L., Poloni, A., Masia, M. C., Salvi, A., & Leoni, P. (1994). Optimization of the yield of PBSC for autotransplantation mobilized by high-dose chemotherapy and G-CSF: Proposal for a mathematical model. Bone Marrow Transplantation, 14(2), 273-278.