Optimized protocol for the extraction of proteins from the human mitral valve

Research output: Contribution to journalArticle

Abstract

Analysis of the cellular proteome can help to elucidate the molecular mechanisms underlying diseases due to the development of technologies that permit the large-scale identification and quantification of the proteins present in complex biological systems. The knowledge gained from a proteomic approach can potentially lead to a better understanding of the pathogenic mechanisms underlying diseases, allowing for the identification of novel diagnostic and prognostic disease markers, and, hopefully, of therapeutic targets. However, the cardiac mitral valve represents a very challenging sample for proteomic analysis because of the low cellularity in proteoglycan and collagen-enriched extracellular matrix. This makes it challenging to extract proteins for a global proteomic analysis. This work describes a protocol that is compatible with subsequent protein analysis, such as quantitative proteomics and immunoblotting. This can allow for the correlation of data concerning protein expression with data on quantitative mRNA expression and non-quantitative immunohistochemical analysis. Indeed, these approaches, when performed together, will lead to a more comprehensive understanding of the molecular mechanisms underlying diseases, from mRNA to posttranslational protein modification. Thus, this method can be relevant to researchers interested in the study of cardiac valve physiopathology.

Original languageEnglish
Article numbere55762
JournalJournal of Visualized Experiments
Volume2017
Issue number124
DOIs
Publication statusPublished - Jun 14 2017

Fingerprint

Mitral Valve
Proteomics
Proteins
Heart Valves
Messenger RNA
Biological systems
Proteoglycans
Proteome
Post Translational Protein Processing
Immunoblotting
Extracellular Matrix
Collagen
Research Personnel
Technology
Therapeutics

Keywords

  • Biochemistry
  • Human normal mitral valve
  • Issue 124
  • Protein analysis
  • Protein extraction
  • Proteomics
  • Tissue homogenization

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Optimized protocol for the extraction of proteins from the human mitral valve. / Banfi, Cristina; Guarino, Anna; Brioschi, Maura; Ghilardi, Stefania; Mastrullo, Valeria; Tremoli, Elena; Polvani, Gianluca.

In: Journal of Visualized Experiments, Vol. 2017, No. 124, e55762, 14.06.2017.

Research output: Contribution to journalArticle

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