Oral lichen planus pathogenesis: A role for the HCV-specific cellular immune response

Massimo Pilli, Amalia Penna, Alessandro Zerbini, Paolo Vescovi, Maddalena Manfredi, Francesco Negro, Marco Carrozzo, Cristina Mori, Tiziana Giuberti, Carlo Ferrari, Gabriele Missale

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Hepatitis C virus infection can be associated with different extrahepatic manifestations, including lichen planus; however, no clear role for HCV in their pathogenesis has been established. T cells were isolated from lichen biopsy specimens of 7 HCV positive patients with oral lichen planus. HCV-specific CD4+ T-cell lines were obtained in 4 patients from lichen lesions but only in 2 of them from the peripheral blood. Different clonal populations were found in oral tissue and peripheral blood of individual patients, as shown by TCR-Vβ analysis of antigen-specific T cellr. Frequency of HCV-specific CD8+ cells tested with 4 different HCV tetramers was significantly higher in the lichen tissue than in the circulation; moreover, lichen-derived HCV-specific CD8+ T cells showed the phenotype of recently activated T cells because most of them were CD69+ and produced interferon gamma (IFN-γ) but expanded poorly in vitro upon antigen stimulation. The specificity of HCV-reactive T-cell recruitment into the lichen tissue was further confirmed by the absence of HBV-specific T cells within lichen lesions in 3 additional patients with lichen planus associated with HBV infection. Our study shows HCV-specific T-cell responses at the site of the lesions of an HCV-associated dermatologic disease, sustained by HCV-specific T cells with phenotypic and functional characteristics of terminally differentiated effector cells. In conclusion, this finding and the detection of HCV RNA strands in the lichen tissue strongly suggest a role for HCV-specific T-cell responses in the pathogenesis of oral lichen planus associated with HCV infection.

Original languageEnglish
Pages (from-to)1446-1452
Number of pages7
JournalHepatology
Volume36
Issue number6
DOIs
Publication statusPublished - Dec 1 2002

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Oral Lichen Planus
Lichens
Cellular Immunity
T-Lymphocytes
Lichen Planus
Viral Tumor Antigens
Virus Diseases
Infection
Hepacivirus
Interferon-gamma
RNA
Phenotype
Biopsy
Antigens
Cell Line

ASJC Scopus subject areas

  • Hepatology

Cite this

Pilli, M., Penna, A., Zerbini, A., Vescovi, P., Manfredi, M., Negro, F., ... Missale, G. (2002). Oral lichen planus pathogenesis: A role for the HCV-specific cellular immune response. Hepatology, 36(6), 1446-1452. https://doi.org/10.1053/jhep.2002.37199

Oral lichen planus pathogenesis : A role for the HCV-specific cellular immune response. / Pilli, Massimo; Penna, Amalia; Zerbini, Alessandro; Vescovi, Paolo; Manfredi, Maddalena; Negro, Francesco; Carrozzo, Marco; Mori, Cristina; Giuberti, Tiziana; Ferrari, Carlo; Missale, Gabriele.

In: Hepatology, Vol. 36, No. 6, 01.12.2002, p. 1446-1452.

Research output: Contribution to journalArticle

Pilli, M, Penna, A, Zerbini, A, Vescovi, P, Manfredi, M, Negro, F, Carrozzo, M, Mori, C, Giuberti, T, Ferrari, C & Missale, G 2002, 'Oral lichen planus pathogenesis: A role for the HCV-specific cellular immune response', Hepatology, vol. 36, no. 6, pp. 1446-1452. https://doi.org/10.1053/jhep.2002.37199
Pilli M, Penna A, Zerbini A, Vescovi P, Manfredi M, Negro F et al. Oral lichen planus pathogenesis: A role for the HCV-specific cellular immune response. Hepatology. 2002 Dec 1;36(6):1446-1452. https://doi.org/10.1053/jhep.2002.37199
Pilli, Massimo ; Penna, Amalia ; Zerbini, Alessandro ; Vescovi, Paolo ; Manfredi, Maddalena ; Negro, Francesco ; Carrozzo, Marco ; Mori, Cristina ; Giuberti, Tiziana ; Ferrari, Carlo ; Missale, Gabriele. / Oral lichen planus pathogenesis : A role for the HCV-specific cellular immune response. In: Hepatology. 2002 ; Vol. 36, No. 6. pp. 1446-1452.
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