Abstract
Background: Testing for oral high-risk human papillomavirus (HPV) DNA may be useful for identifying individuals at increased risk for HPV-driven oropharyngeal cancer (OPC). However, positivity for HPV DNA provides no information on the transforming potential of the infection. In contrast, the detection of high-risk HPV E6/E7 messenger RNA (mRNA) may help to identify clinically significant infections because of the indispensable role of E6/E7 viral oncoproteins in the carcinogenic process. Methods: Oral rinses were collected with a mouthwash from cancer-free individuals at increased risk for oral HPV infection. High-risk HPV DNA and mRNA were evaluated via the testing of the oral rinses with the Linear Array HPV genotyping test and the Aptima HPV assay, respectively. Results: Overall, 310 subjects with no clinical evidence of lesions of the oral cavity and oropharynx were included in the study. Thirty-three (10.6%) harbored high-risk HPV DNA in their oral rinse. These cases, together with 10 random samples negative for high-risk HPV DNA, were tested with the Aptima assay. A valid result was obtained for 41 of the 43 specimens (95.3%). Among the 31 cases that were positive for high-risk HPV DNA and had a valid Aptima result, 4 (12.9%) were positive for HPV mRNA. HPV mRNA was not detected in any of the samples negative for high-risk HPV DNA. Conclusions: HPV mRNA is detectable in oral rinses of cancer-free subjects. Oral HPV mRNA testing may be useful in the screening and/or early detection of HPV-driven OPC by possibly identifying active and transforming oral infections. The testing of individuals at increased risk for HPV-related OPC via simply and noninvasively collected oral specimens is an attractive option for future screening strategies.
| Original language | English |
|---|---|
| Pages (from-to) | 2587-2593 |
| Number of pages | 7 |
| Journal | Cancer |
| Volume | 125 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published - Aug 1 2019 |
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Keywords
- E6/E7 messenger RNA (mRNA)
- head and neck neoplasms
- human papillomavirus
- men who have sex with men (MSM)
- oral rinse
- oropharyngeal neoplasms
ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Oral testing for high-risk human papillomavirus DNA and E6/E7 messenger RNA in healthy individuals at risk for oral infection. / Rollo, Francesca; Pichi, Barbara; Benevolo, Maria; Giuliani, Massimo; Latini, Alessandra; Lorenzon, Laura; Colafigli, Manuela; Frasca, Mirko; Pellini, Raul; Cristaudo, Antonio; Donà, Maria Gabriella.
In: Cancer, Vol. 125, No. 15, 01.08.2019, p. 2587-2593.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oral testing for high-risk human papillomavirus DNA and E6/E7 messenger RNA in healthy individuals at risk for oral infection
AU - Rollo, Francesca
AU - Pichi, Barbara
AU - Benevolo, Maria
AU - Giuliani, Massimo
AU - Latini, Alessandra
AU - Lorenzon, Laura
AU - Colafigli, Manuela
AU - Frasca, Mirko
AU - Pellini, Raul
AU - Cristaudo, Antonio
AU - Donà, Maria Gabriella
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Background: Testing for oral high-risk human papillomavirus (HPV) DNA may be useful for identifying individuals at increased risk for HPV-driven oropharyngeal cancer (OPC). However, positivity for HPV DNA provides no information on the transforming potential of the infection. In contrast, the detection of high-risk HPV E6/E7 messenger RNA (mRNA) may help to identify clinically significant infections because of the indispensable role of E6/E7 viral oncoproteins in the carcinogenic process. Methods: Oral rinses were collected with a mouthwash from cancer-free individuals at increased risk for oral HPV infection. High-risk HPV DNA and mRNA were evaluated via the testing of the oral rinses with the Linear Array HPV genotyping test and the Aptima HPV assay, respectively. Results: Overall, 310 subjects with no clinical evidence of lesions of the oral cavity and oropharynx were included in the study. Thirty-three (10.6%) harbored high-risk HPV DNA in their oral rinse. These cases, together with 10 random samples negative for high-risk HPV DNA, were tested with the Aptima assay. A valid result was obtained for 41 of the 43 specimens (95.3%). Among the 31 cases that were positive for high-risk HPV DNA and had a valid Aptima result, 4 (12.9%) were positive for HPV mRNA. HPV mRNA was not detected in any of the samples negative for high-risk HPV DNA. Conclusions: HPV mRNA is detectable in oral rinses of cancer-free subjects. Oral HPV mRNA testing may be useful in the screening and/or early detection of HPV-driven OPC by possibly identifying active and transforming oral infections. The testing of individuals at increased risk for HPV-related OPC via simply and noninvasively collected oral specimens is an attractive option for future screening strategies.
AB - Background: Testing for oral high-risk human papillomavirus (HPV) DNA may be useful for identifying individuals at increased risk for HPV-driven oropharyngeal cancer (OPC). However, positivity for HPV DNA provides no information on the transforming potential of the infection. In contrast, the detection of high-risk HPV E6/E7 messenger RNA (mRNA) may help to identify clinically significant infections because of the indispensable role of E6/E7 viral oncoproteins in the carcinogenic process. Methods: Oral rinses were collected with a mouthwash from cancer-free individuals at increased risk for oral HPV infection. High-risk HPV DNA and mRNA were evaluated via the testing of the oral rinses with the Linear Array HPV genotyping test and the Aptima HPV assay, respectively. Results: Overall, 310 subjects with no clinical evidence of lesions of the oral cavity and oropharynx were included in the study. Thirty-three (10.6%) harbored high-risk HPV DNA in their oral rinse. These cases, together with 10 random samples negative for high-risk HPV DNA, were tested with the Aptima assay. A valid result was obtained for 41 of the 43 specimens (95.3%). Among the 31 cases that were positive for high-risk HPV DNA and had a valid Aptima result, 4 (12.9%) were positive for HPV mRNA. HPV mRNA was not detected in any of the samples negative for high-risk HPV DNA. Conclusions: HPV mRNA is detectable in oral rinses of cancer-free subjects. Oral HPV mRNA testing may be useful in the screening and/or early detection of HPV-driven OPC by possibly identifying active and transforming oral infections. The testing of individuals at increased risk for HPV-related OPC via simply and noninvasively collected oral specimens is an attractive option for future screening strategies.
KW - E6/E7 messenger RNA (mRNA)
KW - head and neck neoplasms
KW - human papillomavirus
KW - men who have sex with men (MSM)
KW - oral rinse
KW - oropharyngeal neoplasms
UR - http://www.scopus.com/inward/record.url?scp=85065203516&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065203516&partnerID=8YFLogxK
U2 - 10.1002/cncr.32152
DO - 10.1002/cncr.32152
M3 - Article
C2 - 31034590
AN - SCOPUS:85065203516
VL - 125
SP - 2587
EP - 2593
JO - Cancer
JF - Cancer
SN - 0008-543X
IS - 15
ER -