TY - JOUR
T1 - Orbital Rhabdomyosarcoma
T2 - Relationship between DNA Ploidy, P53, Bcl-2, MDR-1 and Ki67 (MIB1) Expression and Clinical Behavior
AU - Staibano, Stefania
AU - Franco, Renato
AU - Tranfa, Fausto
AU - Mezza, Ernesto
AU - Muzio, Lorenzo Lo
AU - Strianese, Diego
AU - Errico, Maria Elena
AU - Bufo, Pantaileo
AU - Ferrara, Gerardo
AU - Somma, Pasquale
AU - Mansueto, Gelsomina
AU - Immacolatagreco, [No Value]
AU - Fiorillo, Amedeo
AU - Bonavolontà, Giulio
AU - De Rosa, Gaetano
PY - 2004/1
Y1 - 2004/1
N2 - Background: As for rhabdomyosarcoma (RMS) of other anatomic regions, the evaluation of traditional clinicopathological parameters does not allow the unequivocal outcome prediction of the single cases of orbital RMS. We investigated the role of DNA ploidy and immunohistochemical expression of p53, bcl-2, MDR-1 and Ki67 (MIB1) in the prognostic evaluation of orbital rhabdomyosarcomas. Materials and Methods: The study population consisted of 11 selected cases. Serial sections of each tumor, stained with Feulgen's technique, were analyzed for the DNA content, using the QUANTIMET 500c Leica analyzer, QWINVO200A software. The results were compared with the immunohistochemical expression of p53 (wild plus mutated, W&M and mutated), bcl2, MDR-1 and Ki67 (MIB1), and with follow-up data. Results: The statistical analysis of results showed that the cases of tetraploid and/or multiploid RMS, overexpressing p53 (W&M and mutated) and MDR-1, were characterized by an overall worse prognosis. On the contrary, the tumors with a favourable clinical course showed hyperexpression of MIB1 and absence of mutated p53 expression. Significantly higher MIB1 expression was found in the relapse-free group of tumors, with respect to the RMS with relapse (both in primary tumors and relative relapses, p0.05). Conclusion: The evaluation of DNA ploidy, p53, MIBI and MDR-1 expression could be used for subtyping of orbital RMS into two prognostically different subcategories, respectively RMS responder to the therapy, with favourable clinical outcome, and RMS with a worse prognosis, requiring more aggressive therapeutic protocols.
AB - Background: As for rhabdomyosarcoma (RMS) of other anatomic regions, the evaluation of traditional clinicopathological parameters does not allow the unequivocal outcome prediction of the single cases of orbital RMS. We investigated the role of DNA ploidy and immunohistochemical expression of p53, bcl-2, MDR-1 and Ki67 (MIB1) in the prognostic evaluation of orbital rhabdomyosarcomas. Materials and Methods: The study population consisted of 11 selected cases. Serial sections of each tumor, stained with Feulgen's technique, were analyzed for the DNA content, using the QUANTIMET 500c Leica analyzer, QWINVO200A software. The results were compared with the immunohistochemical expression of p53 (wild plus mutated, W&M and mutated), bcl2, MDR-1 and Ki67 (MIB1), and with follow-up data. Results: The statistical analysis of results showed that the cases of tetraploid and/or multiploid RMS, overexpressing p53 (W&M and mutated) and MDR-1, were characterized by an overall worse prognosis. On the contrary, the tumors with a favourable clinical course showed hyperexpression of MIB1 and absence of mutated p53 expression. Significantly higher MIB1 expression was found in the relapse-free group of tumors, with respect to the RMS with relapse (both in primary tumors and relative relapses, p0.05). Conclusion: The evaluation of DNA ploidy, p53, MIBI and MDR-1 expression could be used for subtyping of orbital RMS into two prognostically different subcategories, respectively RMS responder to the therapy, with favourable clinical outcome, and RMS with a worse prognosis, requiring more aggressive therapeutic protocols.
KW - bcl2
KW - DNA ploidy
KW - Ki67 (MIB1)
KW - MDR-1
KW - Orbital rhabdomyosarcoma
KW - p53
KW - Prognosis
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M3 - Article
C2 - 15015604
AN - SCOPUS:10744221096
VL - 24
SP - 249
EP - 257
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 1
ER -