TY - JOUR
T1 - Ordering of human bone marrow B lymphocyte precursors by single-cell polymerase chain reaction analyses of the rearrangement status of the immunoglobulin H and L chain gene loci
AU - Ghia, Paolo
AU - Ten Boekel, Edwin
AU - Sanz, Eva
AU - De La Hera, Antonio
AU - Rolink, Antonius
AU - Melchers, Fritz
PY - 1996/12/1
Y1 - 1996/12/1
N2 - CD19+CD10+ human B lineage bone marrow cells were separated into cycling or resting cells, which differ in their expression of CD34, V(preB), recombination activating gene (RAG-1), and terminal deoxynucleotidyl transferase (TdT). Polymerase chain reaction analyses developed for D(H)J(H) and V(K)J(K) V(K)J(K)K((de)) and V(K)K((de)) rearrangements with DNA of single cells and a comparison with B lineage cell development in mouse bone marrow, allow to delineate the human B lymphocyte pathway of development as follows: CD34+V(preB)+RAG-1+TdT+, D(H)J(H)-rearranged, KL germline cycling pre-B I cells → CD34- V(preB)+μH chain+ (pre-B receptor+) RAG- 1+ TdT, V(H)D(H)J(H)-rearranged, KL germline, cycling pre-B II cells → CD34- V(preB)-, intracytoplasmic μH chain+ (pre-B receptor) RAG-1+ TdT, V(H)D(H)J(H)-rearranged, mainly KL germline cycling pre-B II cells → CD34+ V(preB)- intracytoplasmic μH chain+, RAG-1+ TdT, V(H)D(H)J(H)-rearranged, V(K)J(K)-rearranged, IgM-, resting pre-B II cells → CD34+ V(preB)-, sIgM+, RAG-1+ TdT-, V(H)D(H)J(H)- and V(K)J(K)-rearranged IgM+ immature B cells → CD34+, CD10- sIgM+/sIgD+ mature B cells. This order, for the first time established for human B lineage cells, shows striking similarities with that established for mouse B lineage cells in bone marrow.
AB - CD19+CD10+ human B lineage bone marrow cells were separated into cycling or resting cells, which differ in their expression of CD34, V(preB), recombination activating gene (RAG-1), and terminal deoxynucleotidyl transferase (TdT). Polymerase chain reaction analyses developed for D(H)J(H) and V(K)J(K) V(K)J(K)K((de)) and V(K)K((de)) rearrangements with DNA of single cells and a comparison with B lineage cell development in mouse bone marrow, allow to delineate the human B lymphocyte pathway of development as follows: CD34+V(preB)+RAG-1+TdT+, D(H)J(H)-rearranged, KL germline cycling pre-B I cells → CD34- V(preB)+μH chain+ (pre-B receptor+) RAG- 1+ TdT, V(H)D(H)J(H)-rearranged, KL germline, cycling pre-B II cells → CD34- V(preB)-, intracytoplasmic μH chain+ (pre-B receptor) RAG-1+ TdT, V(H)D(H)J(H)-rearranged, mainly KL germline cycling pre-B II cells → CD34+ V(preB)- intracytoplasmic μH chain+, RAG-1+ TdT, V(H)D(H)J(H)-rearranged, V(K)J(K)-rearranged, IgM-, resting pre-B II cells → CD34+ V(preB)-, sIgM+, RAG-1+ TdT-, V(H)D(H)J(H)- and V(K)J(K)-rearranged IgM+ immature B cells → CD34+, CD10- sIgM+/sIgD+ mature B cells. This order, for the first time established for human B lineage cells, shows striking similarities with that established for mouse B lineage cells in bone marrow.
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U2 - 10.1084/jem.184.6.2217
DO - 10.1084/jem.184.6.2217
M3 - Article
C2 - 8976177
AN - SCOPUS:0030442576
VL - 184
SP - 2217
EP - 2229
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 6
ER -