TY - JOUR
T1 - Orderly osteochondral regeneration in a sheep model using a novel nano-composite multilayered biomaterial
AU - Kon, Elizaveta
AU - Delcogliano, Marco
AU - Filardo, Giuseppe
AU - Fini, Milena
AU - Giavaresi, Gianluca
AU - Francioli, Silvia
AU - Martin, Ivan
AU - Pressato, Daniele
AU - Arcangeli, Elena
AU - Quarto, Rodolfo
AU - Sandri, Monica
AU - Marcacci, Maurillo
PY - 2010/1
Y1 - 2010/1
N2 - The objective of this articlewas to investigate the safety and regenerative potential of anewly developed biomimetic scaffoldwhen applied to osteochondral defects in an animal model. A newmultilayer gradient nano-composite scaffold was obtained by nucleating collagen fibrils with hydroxyapatite nanoparticles. In the femoral condyles of 12 sheep, 24 osteochondral lesionswere created.Animalswere randomized into three treatment groups: scaffold alone, scaffold colonized in vitro with autologous chondrocytes and empty defects. Six months after surgery, the animals were sacrificed and the lesions were histologically evaluated. Histologic and gross evaluation of specimens showed good integration of the chondral surface in all groups except for the control group. Significantly better bone regeneration was observed both in the group receiving the scaffold alone and in the group with scaffold loaded with autologous chondrocytes. No difference in cartilage surface reconstruction and osteochondral defect filling was noted between cell-seeded and cell-free groups. In the control group, no bone or cartilage defect healing occurred, and the defects were filled with fibrous tissue. Quantitativemacroscopic and histological score evaluations confirmed the qualitative trends observed. The results of the present study showed that this novel osteochondral scaffold is safe and easy to use, and may represent a suitable matrix to direct and coordinate the process of bone and hyaline-like cartilage regeneration. The comparable regeneration process observed with or without autologous chondrocytes suggests that themainmode of action of the scaffold is based on the recruitment of local cells.
AB - The objective of this articlewas to investigate the safety and regenerative potential of anewly developed biomimetic scaffoldwhen applied to osteochondral defects in an animal model. A newmultilayer gradient nano-composite scaffold was obtained by nucleating collagen fibrils with hydroxyapatite nanoparticles. In the femoral condyles of 12 sheep, 24 osteochondral lesionswere created.Animalswere randomized into three treatment groups: scaffold alone, scaffold colonized in vitro with autologous chondrocytes and empty defects. Six months after surgery, the animals were sacrificed and the lesions were histologically evaluated. Histologic and gross evaluation of specimens showed good integration of the chondral surface in all groups except for the control group. Significantly better bone regeneration was observed both in the group receiving the scaffold alone and in the group with scaffold loaded with autologous chondrocytes. No difference in cartilage surface reconstruction and osteochondral defect filling was noted between cell-seeded and cell-free groups. In the control group, no bone or cartilage defect healing occurred, and the defects were filled with fibrous tissue. Quantitativemacroscopic and histological score evaluations confirmed the qualitative trends observed. The results of the present study showed that this novel osteochondral scaffold is safe and easy to use, and may represent a suitable matrix to direct and coordinate the process of bone and hyaline-like cartilage regeneration. The comparable regeneration process observed with or without autologous chondrocytes suggests that themainmode of action of the scaffold is based on the recruitment of local cells.
KW - Autologous chondrocytes
KW - Biomimetic scaffold
KW - Cartilage regeneration
KW - Osteochondral defect
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U2 - 10.1002/jor.20958
DO - 10.1002/jor.20958
M3 - Article
C2 - 19623663
AN - SCOPUS:73249123534
VL - 28
SP - 116
EP - 124
JO - Journal of Orthopaedic Research
JF - Journal of Orthopaedic Research
SN - 0736-0266
IS - 1
ER -