Organization of extracellular matrix fibers within polyglycolic acid-polylactic acid scaffolds analyzed using x-ray synchrotron-radiation phase-contrast micro computed tomography

Gianni Albertini, Alessandra Giuliani, Vladimir Komlev, Francesca Moroncini, Armanda Pugnaloni, Giuseppina Pennesi, Marzia Belicchi, Corrado Rubini, Franco Rustichelli, Roberta Tasso, Yvan Torrente

Research output: Contribution to journalArticle

Abstract

Spatiotemporal organized patterns of cell surface-associated and extracellular matrix (ECM)-embedded molecules play important roles in the development and functioning of tissues. ECM proteins interact with the surface of bioscaffold polymers and influence material-driven control of cell differentiation., Using X-ray phase-contrast micro computed tomography (microCT), we visualized the three-dimensional (3D) image of ECM organization after in vitro seeding of bone marrow-derived human and murine mesenchymal stem cells (MSCs) induced to myogenic differentiation, labelled with iron oxide nanoparticles, and seeded onto polyglycolic acid-polylactic acid scaffolds. X-ray microCT enabled us to detect with high spatial resolution the 3D structural organization of ECM within the bioscaffold and how the presence of cells modified the construct arrangement. Species-specific differences between the matrix produced by human and murine cells were observed. In conclusion, X-ray synchrotron radiation microCT analysis appeared to be a useful tool to identify the spatiotemporal pattern of organization of ECM fibers within a bioscaffold.

Original languageEnglish
Pages (from-to)403-411
Number of pages9
JournalTissue Engineering - Part C: Methods
Volume15
Issue number3
DOIs
Publication statusPublished - Sep 1 2009

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)
  • Medicine(all)

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