Organoid Models and Applications in Biomedical Research

Christodoulos Xinaris, Valerio Brizi, Giuseppe Remuzzi

Research output: Contribution to journalReview article

Abstract

Recent technical advances in the stem cell field have enabled the in vitro generation of complex structures resembling whole organs termed organoids. Most of these approaches employ three-dimensional (3D) culture systems that allow stem cell-derived or tissue progenitor cells to self-organize into 3D structures. These systems evolved, methodologically and conceptually, from classical reaggregation experiments, showing that dissociated cells from embryonic organs can reaggregate and re-create the original organ architecture. Since organoids can be grown from human stem cells and from patient-derived induced pluripotent stem cells, they create significant prospects for modelling development and diseases, for toxicology and drug discovery studies, and in the field of regenerative medicine. Here, we outline historical advances in the field and describe some of the major recent developments in 3D human organoid formation. Finally, we underline current limitations and highlight examples of how organoid technology can be applied in biomedical research.

Original languageEnglish
Pages (from-to)191-199
Number of pages9
JournalNephron
Volume130
Issue number3
DOIs
Publication statusPublished - 2015

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ASJC Scopus subject areas

  • Medicine(all)

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