Organoids are promising tools for species-specific in vitro toxicological studies

Justyna Augustyniak, Alessia Bertero, Teresa Coccini, Diego Baderna, Leonora Buzanska, Francesca Caloni

Research output: Contribution to journalReview article

Abstract

Organoids are three-dimensional self-aggregating structures generated from stem cells (SCs) or progenitor cells in a process that recapitulates molecular and cellular stages of early organ development. The differentiation process leads to the appearance of specialized mature cells and is connected with changes in the organoid internal structure rearrangement and self-organization. The formation of organ-specific structures in vitro with highly ordered architecture is also strongly influenced by the extracellular matrix. These features make organoids as a powerful model for in vitro toxicology. Nowadays this technology is developing very quickly. In this review we present, from a toxicological and species-specific point of view, the state of the art of organoid generation from adult SCs and pluripotent SCs: embryonic SCs or induced pluripotent SCs. The current culture organoid techniques are discussed for their main advantages, disadvantages and limitations. In the second part of the review, we concentrated on the characterization of species-specific organoids generated from tissue-specific SCs of different sources: mammary (bovine), epidermis (canine), intestinal (porcine, bovine, canine, chicken) and liver (feline, canine).

Original languageEnglish
Pages (from-to)1610-1622
Number of pages13
JournalJournal of Applied Toxicology
Volume39
Issue number12
DOIs
Publication statusPublished - Dec 2019

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