Orientation of the central domains of KSRP and its implications for the interaction with the RNA targets

Irene Díaz-Moreno, David Hollingworth, Geoff Kelly, Stephen Martin, MaríaFlor García-Mayoral, Paola Briata, Roberto Gherzi, Andres Ramos

Research output: Contribution to journalArticle

Abstract

KSRP is a multi-domain RNA-binding protein that recruits the exosome-containing mRNA degradation complex to mRNAs coding for cellular proliferation and inflammatory response factors. The selectivity of this mRNA degradation mechanism relies on KSRP recognition of AU-rich elements in the mRNA 30UTR, that is mediated by KSRP's KH domains. Our structural analysis shows that the inter-domain linker orients the two central KH domains of KSRP-and their RNA-binding surfaces-creating a two-domain unit. We also show that this inter-domain arrangement is important to the interaction with KSRP's RNA targets.

Original languageEnglish
Article numbergkq216
Pages (from-to)5193-5205
Number of pages13
JournalNucleic Acids Research
Volume38
Issue number15
DOIs
Publication statusPublished - Apr 10 2010

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RNA Stability
AU Rich Elements
RNA
Exosomes
Messenger RNA
Cell Proliferation
RNA Recognition Motif Proteins

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Díaz-Moreno, I., Hollingworth, D., Kelly, G., Martin, S., García-Mayoral, M., Briata, P., ... Ramos, A. (2010). Orientation of the central domains of KSRP and its implications for the interaction with the RNA targets. Nucleic Acids Research, 38(15), 5193-5205. [gkq216]. https://doi.org/10.1093/nar/gkq216

Orientation of the central domains of KSRP and its implications for the interaction with the RNA targets. / Díaz-Moreno, Irene; Hollingworth, David; Kelly, Geoff; Martin, Stephen; García-Mayoral, MaríaFlor; Briata, Paola; Gherzi, Roberto; Ramos, Andres.

In: Nucleic Acids Research, Vol. 38, No. 15, gkq216, 10.04.2010, p. 5193-5205.

Research output: Contribution to journalArticle

Díaz-Moreno, I, Hollingworth, D, Kelly, G, Martin, S, García-Mayoral, M, Briata, P, Gherzi, R & Ramos, A 2010, 'Orientation of the central domains of KSRP and its implications for the interaction with the RNA targets', Nucleic Acids Research, vol. 38, no. 15, gkq216, pp. 5193-5205. https://doi.org/10.1093/nar/gkq216
Díaz-Moreno I, Hollingworth D, Kelly G, Martin S, García-Mayoral M, Briata P et al. Orientation of the central domains of KSRP and its implications for the interaction with the RNA targets. Nucleic Acids Research. 2010 Apr 10;38(15):5193-5205. gkq216. https://doi.org/10.1093/nar/gkq216
Díaz-Moreno, Irene ; Hollingworth, David ; Kelly, Geoff ; Martin, Stephen ; García-Mayoral, MaríaFlor ; Briata, Paola ; Gherzi, Roberto ; Ramos, Andres. / Orientation of the central domains of KSRP and its implications for the interaction with the RNA targets. In: Nucleic Acids Research. 2010 ; Vol. 38, No. 15. pp. 5193-5205.
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