Orientation of the central domains of KSRP and its implications for the interaction with the RNA targets

Irene Díaz-Moreno, David Hollingworth, Geoff Kelly, Stephen Martin, MaríaFlor García-Mayoral, Paola Briata, Roberto Gherzi, Andres Ramos

Research output: Contribution to journalArticlepeer-review

Abstract

KSRP is a multi-domain RNA-binding protein that recruits the exosome-containing mRNA degradation complex to mRNAs coding for cellular proliferation and inflammatory response factors. The selectivity of this mRNA degradation mechanism relies on KSRP recognition of AU-rich elements in the mRNA 30UTR, that is mediated by KSRP's KH domains. Our structural analysis shows that the inter-domain linker orients the two central KH domains of KSRP-and their RNA-binding surfaces-creating a two-domain unit. We also show that this inter-domain arrangement is important to the interaction with KSRP's RNA targets.

Original languageEnglish
Article numbergkq216
Pages (from-to)5193-5205
Number of pages13
JournalNucleic Acids Research
Volume38
Issue number15
DOIs
Publication statusPublished - Apr 10 2010

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

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