The possible role of two types of inclusion bodies induced by tomato bushy stunt virus in Gomphrena globosa cells was investigated by electron microscope autoradiography after administration of tritiated uridine and by immunogold labeling on ultrathin sections. Our observations indicate that both multivesicular bodies (MVBs) and associated chloroplast invaginations are involved in virus replication. Since MVBs are always present in infected cells, even in those not containing chloroplasts, they appear to be the main site of virus RNA synthesis, while chloroplast invaginations are likely to provide, when present, an additional vesicular site for the same purpose. Neither of these structures contains viral protein. By contrast, the dense granules are not involved in virus RNA synthesis and consist mainly of virus coat protein, whose production is likely to occur in the cytoplasm.
|Number of pages||8|
|Journal||Journal of Ultrastructure Research and Molecular Structure Research|
|Publication status||Published - 1986|
ASJC Scopus subject areas
- Molecular Biology