Osseointegration is improved by coating titanium implants with a nanostructured thin film with titanium carbide and titanium oxides clustered around graphitic carbon

Francesca Veronesi, Gianluca Giavaresi, Milena Fini, Giovanni Longo, Caterina Alexandra Ioannidu, Anna Scotto d'Abusco, Fabiana Superti, Gianluca Panzini, Carlo Misiano, Alberto Palattella, Paolo Selleri, Nicola Di Girolamo, Viola Garbarino, Laura Politi, Roberto Scandurra

Research output: Contribution to journalArticle

Abstract

Titanium implants coated with a 500 nm nanostructured layer, deposited by the Ion Plating Plasma Assisted (IPPA) technology, composed of 60% graphitic carbon, 25% titanium oxides and 15% titanium carbide were implanted into rabbit femurs whilst into the controlateral femurs uncoated titanium implants were inserted as control. At four time points the animals were injected with calcein green, xylenol orange, oxytetracycline and alizarin. After 2, 4 and 8 weeks femurs were removed and processed for histology and static and dynamic histomorphometry for undecalcified bone processing into methylmethacrylate, sectioned, thinned, polished and stained with Toluidine blue and Fast green. The overall bone-implant contacts rate (percentage of bone-implant contacts/weeks) of the TiC coated implant was 1.6 fold than that of the uncoated titanium implant. The histomorphometric analyses confirmed the histological evaluations. More precisely, higher Mineral Apposition Rate (MAR, μm/day) (p < 0.005) and Bone Formation Rate (BFR, μm2/μm/day) (p < 0.0005) as well as Bone Implant Contact (Bic) and Bone Ingrowth values (p < 0.0005) were observed for the TiC coated implants compared to uncoated implants. In conclusion the hard nanostructured TiC layer protects the bulk titanium implant against the harsh conditions of biological tissues and in the same time, stimulating adhesion, proliferation and activity of osteoblasts, induces a better bone-implant contacts of the implant compared to the uncoated titanium implant.

Original languageEnglish
Pages (from-to)264-271
Number of pages8
JournalMaterials Science and Engineering C
Volume70
Issue numberpt 1
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

titanium carbides
Titanium carbide
Titanium oxides
Titanium
titanium oxides
bones
Bone
Carbon
titanium
coatings
femur
Thin films
Coatings
oxides
carbon
thin films
osteogenesis
osteoblasts
ion plating
Alizarin

Keywords

  • Animal model
  • Graphitic carbon
  • Nanostructured thin film
  • Osseointegration
  • Osteoblasts
  • Titanium carbide

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Osseointegration is improved by coating titanium implants with a nanostructured thin film with titanium carbide and titanium oxides clustered around graphitic carbon. / Veronesi, Francesca; Giavaresi, Gianluca; Fini, Milena; Longo, Giovanni; Ioannidu, Caterina Alexandra; Scotto d'Abusco, Anna; Superti, Fabiana; Panzini, Gianluca; Misiano, Carlo; Palattella, Alberto; Selleri, Paolo; Di Girolamo, Nicola; Garbarino, Viola; Politi, Laura; Scandurra, Roberto.

In: Materials Science and Engineering C, Vol. 70, No. pt 1, 01.01.2017, p. 264-271.

Research output: Contribution to journalArticle

Veronesi, F, Giavaresi, G, Fini, M, Longo, G, Ioannidu, CA, Scotto d'Abusco, A, Superti, F, Panzini, G, Misiano, C, Palattella, A, Selleri, P, Di Girolamo, N, Garbarino, V, Politi, L & Scandurra, R 2017, 'Osseointegration is improved by coating titanium implants with a nanostructured thin film with titanium carbide and titanium oxides clustered around graphitic carbon', Materials Science and Engineering C, vol. 70, no. pt 1, pp. 264-271. https://doi.org/10.1016/j.msec.2016.08.076
Veronesi, Francesca ; Giavaresi, Gianluca ; Fini, Milena ; Longo, Giovanni ; Ioannidu, Caterina Alexandra ; Scotto d'Abusco, Anna ; Superti, Fabiana ; Panzini, Gianluca ; Misiano, Carlo ; Palattella, Alberto ; Selleri, Paolo ; Di Girolamo, Nicola ; Garbarino, Viola ; Politi, Laura ; Scandurra, Roberto. / Osseointegration is improved by coating titanium implants with a nanostructured thin film with titanium carbide and titanium oxides clustered around graphitic carbon. In: Materials Science and Engineering C. 2017 ; Vol. 70, No. pt 1. pp. 264-271.
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AU - Giavaresi, Gianluca

AU - Fini, Milena

AU - Longo, Giovanni

AU - Ioannidu, Caterina Alexandra

AU - Scotto d'Abusco, Anna

AU - Superti, Fabiana

AU - Panzini, Gianluca

AU - Misiano, Carlo

AU - Palattella, Alberto

AU - Selleri, Paolo

AU - Di Girolamo, Nicola

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AU - Scandurra, Roberto

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N2 - Titanium implants coated with a 500 nm nanostructured layer, deposited by the Ion Plating Plasma Assisted (IPPA) technology, composed of 60% graphitic carbon, 25% titanium oxides and 15% titanium carbide were implanted into rabbit femurs whilst into the controlateral femurs uncoated titanium implants were inserted as control. At four time points the animals were injected with calcein green, xylenol orange, oxytetracycline and alizarin. After 2, 4 and 8 weeks femurs were removed and processed for histology and static and dynamic histomorphometry for undecalcified bone processing into methylmethacrylate, sectioned, thinned, polished and stained with Toluidine blue and Fast green. The overall bone-implant contacts rate (percentage of bone-implant contacts/weeks) of the TiC coated implant was 1.6 fold than that of the uncoated titanium implant. The histomorphometric analyses confirmed the histological evaluations. More precisely, higher Mineral Apposition Rate (MAR, μm/day) (p < 0.005) and Bone Formation Rate (BFR, μm2/μm/day) (p < 0.0005) as well as Bone Implant Contact (Bic) and Bone Ingrowth values (p < 0.0005) were observed for the TiC coated implants compared to uncoated implants. In conclusion the hard nanostructured TiC layer protects the bulk titanium implant against the harsh conditions of biological tissues and in the same time, stimulating adhesion, proliferation and activity of osteoblasts, induces a better bone-implant contacts of the implant compared to the uncoated titanium implant.

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