Osteoarthritis treated with mesenchymal stem cells on Hyaluronan-based scaffold in rabbit

Brunella Grigolo, Gina Lisignoli, Giovanna Desando, Carola Cavallo, Emanuele Marconi, Matilde Tschon, Gianluca Giavaresi, Milena Fini, Roberto Giardino, Andrea Facchini

Research output: Contribution to journalArticle

Abstract

Objective: Osteoarthritis (OA) is a disease that limits the mobility of patients and is of considerable economical importance. Up to now, despite the increasing number of patients with OA, treatments to manage the disease remain symptomatic, designed to control pain, and improve function and quality of life limiting adverse events. With the aim to explore a new approach to treat OA patients suffering from early degenerative lesions of hyaline cartilage, we transplanted in an experimental animal model of OA a hyaluronan-based scaffold (Hyaff®11) seeded with mesenchymal stem cells (MSCs) obtained from bone marrow and expanded in culture. Design: Rabbit knee joints were bilaterally subjected to anterior cruciate ligament transection to surgically induce OA. After 8 weeks, the time necessary to the development of cartilage surface damage, animals were treated with MSCs seeded onto Hyaff-11 scaffold in the left condyle and unseeded Hyaff-11 in the controlateral knee. Untreated rabbits were used as controls. All the animals were sacrificed at 3 and 6 months after surgery. Histological, histomorphometric, and immunohistological evaluations were performed. Results: OA changes developed in all animals subjected to anterior cruciate ligament transection. The predominant macroscopically observed OA changes were mild (lateral femoral condyle) or moderate (medial femoral condyle) ulcerations. Statistically significant differences in the quality of the regenerated tissue were found between the implants with scaffolds carrying MSCs compared to the scaffold alone or controls in particular at 6 months. Conclusions: From the observations, it is possible to demonstrate that Hyaff-11, a hyaluronan-based scaffold, has potential for MSC implantation and that may have application for the treatment of early OA in humans.

Original languageEnglish
Pages (from-to)647-658
Number of pages12
JournalTissue Engineering - Part C: Methods
Volume15
Issue number4
DOIs
Publication statusPublished - Dec 1 2009

Fingerprint

Hyaluronic Acid
Stem cells
Mesenchymal Stromal Cells
Scaffolds
Osteoarthritis
Animals
Rabbits
Ligaments
Cartilage
Anterior Cruciate Ligament
Scaffolds (biology)
Thigh
Bone and Bones
Surgery
Bone
Tissue
Hyaline Cartilage
Knee Joint
Knee
Animal Models

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

Cite this

Osteoarthritis treated with mesenchymal stem cells on Hyaluronan-based scaffold in rabbit. / Grigolo, Brunella; Lisignoli, Gina; Desando, Giovanna; Cavallo, Carola; Marconi, Emanuele; Tschon, Matilde; Giavaresi, Gianluca; Fini, Milena; Giardino, Roberto; Facchini, Andrea.

In: Tissue Engineering - Part C: Methods, Vol. 15, No. 4, 01.12.2009, p. 647-658.

Research output: Contribution to journalArticle

Grigolo, Brunella ; Lisignoli, Gina ; Desando, Giovanna ; Cavallo, Carola ; Marconi, Emanuele ; Tschon, Matilde ; Giavaresi, Gianluca ; Fini, Milena ; Giardino, Roberto ; Facchini, Andrea. / Osteoarthritis treated with mesenchymal stem cells on Hyaluronan-based scaffold in rabbit. In: Tissue Engineering - Part C: Methods. 2009 ; Vol. 15, No. 4. pp. 647-658.
@article{104b0476fd044ecbb5e60b9cc9401cb6,
title = "Osteoarthritis treated with mesenchymal stem cells on Hyaluronan-based scaffold in rabbit",
abstract = "Objective: Osteoarthritis (OA) is a disease that limits the mobility of patients and is of considerable economical importance. Up to now, despite the increasing number of patients with OA, treatments to manage the disease remain symptomatic, designed to control pain, and improve function and quality of life limiting adverse events. With the aim to explore a new approach to treat OA patients suffering from early degenerative lesions of hyaline cartilage, we transplanted in an experimental animal model of OA a hyaluronan-based scaffold (Hyaff{\circledR}11) seeded with mesenchymal stem cells (MSCs) obtained from bone marrow and expanded in culture. Design: Rabbit knee joints were bilaterally subjected to anterior cruciate ligament transection to surgically induce OA. After 8 weeks, the time necessary to the development of cartilage surface damage, animals were treated with MSCs seeded onto Hyaff-11 scaffold in the left condyle and unseeded Hyaff-11 in the controlateral knee. Untreated rabbits were used as controls. All the animals were sacrificed at 3 and 6 months after surgery. Histological, histomorphometric, and immunohistological evaluations were performed. Results: OA changes developed in all animals subjected to anterior cruciate ligament transection. The predominant macroscopically observed OA changes were mild (lateral femoral condyle) or moderate (medial femoral condyle) ulcerations. Statistically significant differences in the quality of the regenerated tissue were found between the implants with scaffolds carrying MSCs compared to the scaffold alone or controls in particular at 6 months. Conclusions: From the observations, it is possible to demonstrate that Hyaff-11, a hyaluronan-based scaffold, has potential for MSC implantation and that may have application for the treatment of early OA in humans.",
author = "Brunella Grigolo and Gina Lisignoli and Giovanna Desando and Carola Cavallo and Emanuele Marconi and Matilde Tschon and Gianluca Giavaresi and Milena Fini and Roberto Giardino and Andrea Facchini",
year = "2009",
month = "12",
day = "1",
doi = "10.1089/ten.tec.2008.0569",
language = "English",
volume = "15",
pages = "647--658",
journal = "Tissue Engineering - Part C: Methods",
issn = "1937-3384",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - Osteoarthritis treated with mesenchymal stem cells on Hyaluronan-based scaffold in rabbit

AU - Grigolo, Brunella

AU - Lisignoli, Gina

AU - Desando, Giovanna

AU - Cavallo, Carola

AU - Marconi, Emanuele

AU - Tschon, Matilde

AU - Giavaresi, Gianluca

AU - Fini, Milena

AU - Giardino, Roberto

AU - Facchini, Andrea

PY - 2009/12/1

Y1 - 2009/12/1

N2 - Objective: Osteoarthritis (OA) is a disease that limits the mobility of patients and is of considerable economical importance. Up to now, despite the increasing number of patients with OA, treatments to manage the disease remain symptomatic, designed to control pain, and improve function and quality of life limiting adverse events. With the aim to explore a new approach to treat OA patients suffering from early degenerative lesions of hyaline cartilage, we transplanted in an experimental animal model of OA a hyaluronan-based scaffold (Hyaff®11) seeded with mesenchymal stem cells (MSCs) obtained from bone marrow and expanded in culture. Design: Rabbit knee joints were bilaterally subjected to anterior cruciate ligament transection to surgically induce OA. After 8 weeks, the time necessary to the development of cartilage surface damage, animals were treated with MSCs seeded onto Hyaff-11 scaffold in the left condyle and unseeded Hyaff-11 in the controlateral knee. Untreated rabbits were used as controls. All the animals were sacrificed at 3 and 6 months after surgery. Histological, histomorphometric, and immunohistological evaluations were performed. Results: OA changes developed in all animals subjected to anterior cruciate ligament transection. The predominant macroscopically observed OA changes were mild (lateral femoral condyle) or moderate (medial femoral condyle) ulcerations. Statistically significant differences in the quality of the regenerated tissue were found between the implants with scaffolds carrying MSCs compared to the scaffold alone or controls in particular at 6 months. Conclusions: From the observations, it is possible to demonstrate that Hyaff-11, a hyaluronan-based scaffold, has potential for MSC implantation and that may have application for the treatment of early OA in humans.

AB - Objective: Osteoarthritis (OA) is a disease that limits the mobility of patients and is of considerable economical importance. Up to now, despite the increasing number of patients with OA, treatments to manage the disease remain symptomatic, designed to control pain, and improve function and quality of life limiting adverse events. With the aim to explore a new approach to treat OA patients suffering from early degenerative lesions of hyaline cartilage, we transplanted in an experimental animal model of OA a hyaluronan-based scaffold (Hyaff®11) seeded with mesenchymal stem cells (MSCs) obtained from bone marrow and expanded in culture. Design: Rabbit knee joints were bilaterally subjected to anterior cruciate ligament transection to surgically induce OA. After 8 weeks, the time necessary to the development of cartilage surface damage, animals were treated with MSCs seeded onto Hyaff-11 scaffold in the left condyle and unseeded Hyaff-11 in the controlateral knee. Untreated rabbits were used as controls. All the animals were sacrificed at 3 and 6 months after surgery. Histological, histomorphometric, and immunohistological evaluations were performed. Results: OA changes developed in all animals subjected to anterior cruciate ligament transection. The predominant macroscopically observed OA changes were mild (lateral femoral condyle) or moderate (medial femoral condyle) ulcerations. Statistically significant differences in the quality of the regenerated tissue were found between the implants with scaffolds carrying MSCs compared to the scaffold alone or controls in particular at 6 months. Conclusions: From the observations, it is possible to demonstrate that Hyaff-11, a hyaluronan-based scaffold, has potential for MSC implantation and that may have application for the treatment of early OA in humans.

UR - http://www.scopus.com/inward/record.url?scp=68949107050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68949107050&partnerID=8YFLogxK

U2 - 10.1089/ten.tec.2008.0569

DO - 10.1089/ten.tec.2008.0569

M3 - Article

C2 - 19249964

AN - SCOPUS:68949107050

VL - 15

SP - 647

EP - 658

JO - Tissue Engineering - Part C: Methods

JF - Tissue Engineering - Part C: Methods

SN - 1937-3384

IS - 4

ER -