Osteogenic differentiation and gene expression of dental pulp stem cells under low-level laser irradiation: a good promise for tissue engineering

A. Ballini, F. Mastrangelo, G. Gastaldi, L. Tettamanti, N. Bukvic, S. Cantore, T. Cocco, R. Saini, A. Desiate, E. Gherlone, S. Scacco

Research output: Contribution to journalArticle

Abstract

The effects of low-level laser therapy (LLLT) has been the focus of recent studies as being assumed responsible for promoting photostimulatory and photobiomodulatory effects in vivo and in vitro, increasing cell metabolism, improving cell regeneration and invoking an anti-inflammatory response. A positive effect of LLLT on the bone proliferation of some cell types has been observed, but little is known about its effect on dental pulp stem cells (DPSCs). Here, we accurately describe the technical procedure to isolate mesenchymal DPSCs, and assay their osteogenic capacity when irradiated with an LLLT source. These preliminary results show that LLLT irradiation influences the in vitro proliferation of DPSCs and increases the expression of essential proteins for bone formation, although it is necessary to carry out further experiments on other cell types and to uniform the methodological designs.

Original languageEnglish
Pages (from-to)813-822
Number of pages10
JournalJournal of Biological Regulators and Homeostatic Agents
Volume29
Issue number4
Publication statusPublished - Oct 1 2015

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Dental Pulp
Tissue Engineering
Lasers
Stem Cells
Gene Expression
Colony-Forming Units Assay
Osteogenesis
Regeneration
Anti-Inflammatory Agents
Cell Proliferation
Bone and Bones
Low-Level Light Therapy
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Osteogenic differentiation and gene expression of dental pulp stem cells under low-level laser irradiation : a good promise for tissue engineering. / Ballini, A.; Mastrangelo, F.; Gastaldi, G.; Tettamanti, L.; Bukvic, N.; Cantore, S.; Cocco, T.; Saini, R.; Desiate, A.; Gherlone, E.; Scacco, S.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 29, No. 4, 01.10.2015, p. 813-822.

Research output: Contribution to journalArticle

Ballini, A. ; Mastrangelo, F. ; Gastaldi, G. ; Tettamanti, L. ; Bukvic, N. ; Cantore, S. ; Cocco, T. ; Saini, R. ; Desiate, A. ; Gherlone, E. ; Scacco, S. / Osteogenic differentiation and gene expression of dental pulp stem cells under low-level laser irradiation : a good promise for tissue engineering. In: Journal of Biological Regulators and Homeostatic Agents. 2015 ; Vol. 29, No. 4. pp. 813-822.
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