Osteogenic effects of an electromagnetic stimulation on human mesenchymal stromal cells seeded onto gelatin cryogel

Lorenzo Fassina, Enrica Saino, Sandra Van Vlierberghe, Jorg Schelfhout, Maria Antonietta Avanzini, Peter Dubruel, Francesco Benazzo, Giovanni Magenes, Livia Visai

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Bone tissue engineering typically uses biomaterial scaffolds, osteoblasts or cells that can become osteoblasts, and biophysical stimulations to promote cell attachment and differentiation. In this study, we investigated the effects of an electromagnetic wave on mesenchymal stromal cells isolated from the bone marrow and seeded upon gelatin cryogel disks. In comparison with control conditions without electromagnetic stimulus, the electromagnetic treatment (magnetic field, 2 mT; frequency, 75 Hz) increased the cell proliferation and differentiation and enhanced the biomaterial surface coating with bone extracellular matrix proteins (osteocalcin, osteopontin, and type-I collagen). Using this tissue-engineering approach, the gelatin biomaterial, coated with differentiated cells and their extracellular matrix proteins, may be used in clinical applications as an implant for bone defect repair.

Original languageEnglish
Title of host publicationOsteocalcin: Production, Regulation and Disease
PublisherNova Science Publishers, Inc.
Pages29-42
Number of pages14
ISBN (Print)9781619420472
Publication statusPublished - Jan 2012

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Keywords

  • Bone matrix proteins
  • Bovine gelatin cryogel
  • Electromagnetic bioreactor
  • Mesenchymal stromal cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fassina, L., Saino, E., Vlierberghe, S. V., Schelfhout, J., Avanzini, M. A., Dubruel, P., Benazzo, F., Magenes, G., & Visai, L. (2012). Osteogenic effects of an electromagnetic stimulation on human mesenchymal stromal cells seeded onto gelatin cryogel. In Osteocalcin: Production, Regulation and Disease (pp. 29-42). Nova Science Publishers, Inc..