Osteogenic effects of an electromagnetic stimulation on human mesenchymal stromal cells seeded onto gelatin cryogel

Lorenzo Fassina, Enrica Saino, Sandra Van Vlierberghe, Jorg Schelfhout, Maria Antonietta Avanzini, Peter Dubruel, Francesco Benazzo, Giovanni Magenes, Livia Visai

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Bone tissue engineering typically uses biomaterial scaffolds, osteoblasts or cells that can become osteoblasts, and biophysical stimulations to promote cell attachment and differentiation. In this study, we investigated the effects of an electromagnetic wave on mesenchymal stromal cells isolated from the bone marrow and seeded upon gelatin cryogel disks. In comparison with control conditions without electromagnetic stimulus, the electromagnetic treatment (magnetic field, 2 mT; frequency, 75 Hz) increased the cell proliferation and differentiation and enhanced the biomaterial surface coating with bone extracellular matrix proteins (osteocalcin, osteopontin, and type-I collagen). Using this tissue-engineering approach, the gelatin biomaterial, coated with differentiated cells and their extracellular matrix proteins, may be used in clinical applications as an implant for bone defect repair.

Original languageEnglish
Title of host publicationOsteocalcin: Production, Regulation and Disease
PublisherNova Science Publishers, Inc.
Pages29-42
Number of pages14
ISBN (Print)9781619420472
Publication statusPublished - Jan 2012

Fingerprint

Cryogels
Electromagnetic Phenomena
Biocompatible Materials
Gelatin
Mesenchymal Stromal Cells
Bone
Extracellular Matrix Proteins
Tissue Engineering
Osteoblasts
Cell Differentiation
Tissue engineering
Electromagnetic Radiation
Bone and Bones
Bone Matrix
Osteopontin
Osteocalcin
Magnetic Fields
Collagen Type I
Cell proliferation
Scaffolds (biology)

Keywords

  • Bone matrix proteins
  • Bovine gelatin cryogel
  • Electromagnetic bioreactor
  • Mesenchymal stromal cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fassina, L., Saino, E., Vlierberghe, S. V., Schelfhout, J., Avanzini, M. A., Dubruel, P., ... Visai, L. (2012). Osteogenic effects of an electromagnetic stimulation on human mesenchymal stromal cells seeded onto gelatin cryogel. In Osteocalcin: Production, Regulation and Disease (pp. 29-42). Nova Science Publishers, Inc..

Osteogenic effects of an electromagnetic stimulation on human mesenchymal stromal cells seeded onto gelatin cryogel. / Fassina, Lorenzo; Saino, Enrica; Vlierberghe, Sandra Van; Schelfhout, Jorg; Avanzini, Maria Antonietta; Dubruel, Peter; Benazzo, Francesco; Magenes, Giovanni; Visai, Livia.

Osteocalcin: Production, Regulation and Disease. Nova Science Publishers, Inc., 2012. p. 29-42.

Research output: Chapter in Book/Report/Conference proceedingChapter

Fassina, L, Saino, E, Vlierberghe, SV, Schelfhout, J, Avanzini, MA, Dubruel, P, Benazzo, F, Magenes, G & Visai, L 2012, Osteogenic effects of an electromagnetic stimulation on human mesenchymal stromal cells seeded onto gelatin cryogel. in Osteocalcin: Production, Regulation and Disease. Nova Science Publishers, Inc., pp. 29-42.
Fassina L, Saino E, Vlierberghe SV, Schelfhout J, Avanzini MA, Dubruel P et al. Osteogenic effects of an electromagnetic stimulation on human mesenchymal stromal cells seeded onto gelatin cryogel. In Osteocalcin: Production, Regulation and Disease. Nova Science Publishers, Inc. 2012. p. 29-42
Fassina, Lorenzo ; Saino, Enrica ; Vlierberghe, Sandra Van ; Schelfhout, Jorg ; Avanzini, Maria Antonietta ; Dubruel, Peter ; Benazzo, Francesco ; Magenes, Giovanni ; Visai, Livia. / Osteogenic effects of an electromagnetic stimulation on human mesenchymal stromal cells seeded onto gelatin cryogel. Osteocalcin: Production, Regulation and Disease. Nova Science Publishers, Inc., 2012. pp. 29-42
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