Osteoinduction of human mesenchymal stem cells by bioactive composite scaffolds without supplemental osteogenic growth factors

Alessandro Polini, Dario Pisignano, Manuela Parodi, Rodolfo Quarto, Silvia Scaglione

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

The development of a new family of implantable bioinspired materials is a focal point of bone tissue engineering. Implant surfaces that better mimic the natural bone extracellular matrix, a naturally nano-composite tissue, can stimulate stem cell differentiation towards osteogenic lineages in the absence of specific chemical treatments. Herein we describe a bioactive composite nanofibrous scaffold, composed of poly-caprolactone (PCL) and nano-sized hydroxyapatite (HA) or beta-tricalcium phosphate (TCP), which was able to support the growth of human bone marrow mesenchymal stem cells (hMSCs) and guide their osteogenic differentiation at the same time. Morphological and physical/chemical investigations were carried out by scanning, transmission electron microscopy, Fourier-transform infrared (FTIR) spectroscopy, mechanical and wettability analysis. Upon culturing hMSCs on composite nanofibers, we found that the incorporation of either HA or TCP into the PCL nanofibers did not affect cell viability, meanwhile the presence of the mineral phase increases the activity of alkaline phosphatase (ALP), an early marker of bone formation, and mRNA expression levels of osteoblast-related genes, such as the Runt-related transcription factor 2 (Runx-2) and bone sialoprotein (BSP), in total absence of osteogenic supplements. These results suggest that both the nanofibrous structure and the chemical composition of the scaffolds play a role in regulating the osteogenic differentiation of hMSCs.

Original languageEnglish
Article numbere26211
JournalPLoS One
Volume6
Issue number10
DOIs
Publication statusPublished - Oct 12 2011

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composite materials
Stem cells
Mesenchymal Stromal Cells
Scaffolds
growth factors
Nanofibers
stem cells
Intercellular Signaling Peptides and Proteins
bone marrow cells
Bone
Bone Marrow
nanofibers
tricalcium phosphate
hydroxyapatite
Composite materials
bones
Integrin-Binding Sialoprotein
Wettability
Scanning Transmission Electron Microscopy
Bone Matrix

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Osteoinduction of human mesenchymal stem cells by bioactive composite scaffolds without supplemental osteogenic growth factors. / Polini, Alessandro; Pisignano, Dario; Parodi, Manuela; Quarto, Rodolfo; Scaglione, Silvia.

In: PLoS One, Vol. 6, No. 10, e26211, 12.10.2011.

Research output: Contribution to journalArticle

Polini, Alessandro ; Pisignano, Dario ; Parodi, Manuela ; Quarto, Rodolfo ; Scaglione, Silvia. / Osteoinduction of human mesenchymal stem cells by bioactive composite scaffolds without supplemental osteogenic growth factors. In: PLoS One. 2011 ; Vol. 6, No. 10.
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