Osteonectin transcript and metastatic behavior in v-Ki-ras transformed fibroblasts

M. P. Colombo, G. Biondi, D. Galasso, P. Baracetti, C. C. Howe, G. Parmiani

Research output: Contribution to journalArticle

Abstract

Osteonectin is one of the major non-collagenous proteins of bone. However, its transcript has been found in many soft, extracellular matrix-producing tissues; an osteonectin-related protein was detected in tumor basement membrane. We have investigated the expression of osteonectin gene in fresh BALB/c fibroblasts transformed by v-Ki-ras. Transformed cells exhibited lower levels of RNA as compared with normal fibroblasts. The transformed cells were cloned after in vivo tumorigenic assay, and 4 clones were analyzed for osteonectin expression by Northern blots. Two of them were selected for high or low osteonectin expression and tested in vivo in spontaneous and artificial metastasis assays. High osteonectin expression was correlated with high lung colonization. When 105 cells were injected i.v., median colony value was 55 and 20 in higher expressor vs. lower expressor respectively (p

Original languageEnglish
Pages (from-to)76-77
Number of pages2
JournalInternational Journal of Cancer
Issue numberSUPPL. 4
Publication statusPublished - 1989

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Osteonectin
Fibroblasts
Basement Membrane
Northern Blotting
Extracellular Matrix
Proteins
Clone Cells
RNA
Neoplasm Metastasis
Gene Expression
Bone and Bones
Lung

ASJC Scopus subject areas

  • Cancer Research
  • Medicine(all)
  • Oncology

Cite this

Colombo, M. P., Biondi, G., Galasso, D., Baracetti, P., Howe, C. C., & Parmiani, G. (1989). Osteonectin transcript and metastatic behavior in v-Ki-ras transformed fibroblasts. International Journal of Cancer, (SUPPL. 4), 76-77.

Osteonectin transcript and metastatic behavior in v-Ki-ras transformed fibroblasts. / Colombo, M. P.; Biondi, G.; Galasso, D.; Baracetti, P.; Howe, C. C.; Parmiani, G.

In: International Journal of Cancer, No. SUPPL. 4, 1989, p. 76-77.

Research output: Contribution to journalArticle

Colombo, MP, Biondi, G, Galasso, D, Baracetti, P, Howe, CC & Parmiani, G 1989, 'Osteonectin transcript and metastatic behavior in v-Ki-ras transformed fibroblasts', International Journal of Cancer, no. SUPPL. 4, pp. 76-77.
Colombo MP, Biondi G, Galasso D, Baracetti P, Howe CC, Parmiani G. Osteonectin transcript and metastatic behavior in v-Ki-ras transformed fibroblasts. International Journal of Cancer. 1989;(SUPPL. 4):76-77.
Colombo, M. P. ; Biondi, G. ; Galasso, D. ; Baracetti, P. ; Howe, C. C. ; Parmiani, G. / Osteonectin transcript and metastatic behavior in v-Ki-ras transformed fibroblasts. In: International Journal of Cancer. 1989 ; No. SUPPL. 4. pp. 76-77.
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