Abstract
Osteonectin is one of the major non‐collagenous proteins of bone. However, its transcript has been found in many soft, extracellular matrix‐producing tissues; an osteonectin‐related protein was detected in tumor basement membrane. We have investigated the expression of osteonectin gene in fresh BALB/c fibroblasts transformed by v‐Ki‐ras. Transformed cells exhibited lower levels of RNA as compared with normal fibroblasts. The transformed cells were cloned after in vivo tumorigenic assay, and 4 clones were analyzed for osteonectin expression by Northern blots. Two of them were selected for high or low osteonectin expression and tested in vivo in spontaneous and artificial metastasis assays. High osteonectin expression was correlated with high lung colonization. When 105 cells were injected i.v., median colony value was 55 and 20 in higher expressor vs. lower expressor respectively (p < 0.005). Spontaneous metastasis indicates a possible reverse correlation. Our data align osteonectin with other matrix‐components and adhesion molecules in affecting potential metastatic spreading of transformed cells.
| Original language | English |
|---|---|
| Pages (from-to) | 76-77 |
| Number of pages | 2 |
| Journal | International Journal of Cancer |
| Volume | 44 |
| Issue number | 1 S |
| DOIs | |
| Publication status | Published - 1989 |
Fingerprint
ASJC Scopus subject areas
- Medicine(all)
- Oncology
- Cancer Research
Cite this
Osteonectin transcript and metastatic behavior in v‐Ki‐ras transformed fibroblasts. / Colombo, M. P.; Biondi, G.; Galasso, D.; Baracetti, P.; Howe, C. C.; Parmiani, G.
In: International Journal of Cancer, Vol. 44, No. 1 S, 1989, p. 76-77.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Osteonectin transcript and metastatic behavior in v‐Ki‐ras transformed fibroblasts
AU - Colombo, M. P.
AU - Biondi, G.
AU - Galasso, D.
AU - Baracetti, P.
AU - Howe, C. C.
AU - Parmiani, G.
PY - 1989
Y1 - 1989
N2 - Osteonectin is one of the major non‐collagenous proteins of bone. However, its transcript has been found in many soft, extracellular matrix‐producing tissues; an osteonectin‐related protein was detected in tumor basement membrane. We have investigated the expression of osteonectin gene in fresh BALB/c fibroblasts transformed by v‐Ki‐ras. Transformed cells exhibited lower levels of RNA as compared with normal fibroblasts. The transformed cells were cloned after in vivo tumorigenic assay, and 4 clones were analyzed for osteonectin expression by Northern blots. Two of them were selected for high or low osteonectin expression and tested in vivo in spontaneous and artificial metastasis assays. High osteonectin expression was correlated with high lung colonization. When 105 cells were injected i.v., median colony value was 55 and 20 in higher expressor vs. lower expressor respectively (p < 0.005). Spontaneous metastasis indicates a possible reverse correlation. Our data align osteonectin with other matrix‐components and adhesion molecules in affecting potential metastatic spreading of transformed cells.
AB - Osteonectin is one of the major non‐collagenous proteins of bone. However, its transcript has been found in many soft, extracellular matrix‐producing tissues; an osteonectin‐related protein was detected in tumor basement membrane. We have investigated the expression of osteonectin gene in fresh BALB/c fibroblasts transformed by v‐Ki‐ras. Transformed cells exhibited lower levels of RNA as compared with normal fibroblasts. The transformed cells were cloned after in vivo tumorigenic assay, and 4 clones were analyzed for osteonectin expression by Northern blots. Two of them were selected for high or low osteonectin expression and tested in vivo in spontaneous and artificial metastasis assays. High osteonectin expression was correlated with high lung colonization. When 105 cells were injected i.v., median colony value was 55 and 20 in higher expressor vs. lower expressor respectively (p < 0.005). Spontaneous metastasis indicates a possible reverse correlation. Our data align osteonectin with other matrix‐components and adhesion molecules in affecting potential metastatic spreading of transformed cells.
UR - http://www.scopus.com/inward/record.url?scp=84991138912&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991138912&partnerID=8YFLogxK
U2 - 10.1002/ijc.2910440720
DO - 10.1002/ijc.2910440720
M3 - Article
AN - SCOPUS:84991138912
VL - 44
SP - 76
EP - 77
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 1 S
ER -