Osteopontin is an autoantigen of the somatostatin cells in human islets: Identification by screening random peptide libraries with sera of patients with insulin-dependent diabetes mellitus

Alessandra Fierabracci, Paul Andrew Biro, Yiangos Yiangou, Carmela Mennuni, Alessandra Luzzago, J. Ludvigsson, Riccardo Cortese, Gian Franco Bottazzo

Research output: Contribution to journalArticlepeer-review

Abstract

Random peptide libraries (RPLs) screening with IDDM sera has identified 5 disease-specific 'mimotopes' displayed on phage (phagotopes). We characterised one phagotope (CH1p), by raising a rabbit antibody against the peptide insert on phage, which was employed in immunohistochemistry, Western blotting and cDNA libraries screening. The CH1p mimotope was detected in somatostatin cells of human islets and experimentally raised anti-osteopontin antibodies or human sera positive for the phagotope, detected a similar subpopulation of islet cells. The screening of cDNA library identified a clone corresponding to human osteopontin. In summary, RPLs proved to be successful in the identification of a novel islet-related autoantigen (osteopontin), whose significance in disease remains to be established. Copyright (C) 1999.

Original languageEnglish
Pages (from-to)342-354
Number of pages13
JournalVaccine
Volume18
Issue number3-4
DOIs
Publication statusPublished - Sep 1999

Keywords

  • Autoantigens
  • Insulin-dependent diabetes mellitus (IDDM)
  • Random peptide libraries (RPLs)

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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