Otx1 null mutant mice show partial segregation of sensory epithelia comparable to lamprey ears

B. Fritzsch, M. Signore, A. Simeone

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We investigated the development of inner ear innervation in Otx1 null mutants, which lack a horizontal canal, between embryonic day 12 (E12) and postnatal day 7 (P7) with DiI and immunostaining for acetylated tubulin. Comparable to control animals, horizontal crista-like fibers were found to cross over the utricle in Otx1 null mice. In mutants these fibers extend toward an area near the endolymphatic duct, not to a horizontal crista. Most Otx1 null mutants had a small patch of sensory hair cells at this position. Measurement of the area of the utricular macula suggested it to be enlarged in Otx1 null mutants. We suggest that parts of the horizontal canal crista remain incorporated in the utricular sensory epithelium in Otx1 null mutants. Other parts of the horizontal crista appear to be variably segregated to form the isolated patch of hair cells identifiable by the unique fiber trajectory as representing the horizontal canal crista. Comparison with lamprey ear innervation reveals similarities in the pattern of innervation with the dorsal macula, a sensory patch of unknown function. SEM data confirm that all foramina are less constricted in Otx1 null mutants. We propose that Otx1 is not directly involved in sensory hair cell formation of the horizontal canal but affects the segregation of the horizontal canal crista from the utricle. It also affects constriction of the two main foramina in the ear, but not their initial formation. Otx1 is thus causally related to horizontal canal morphogenesis as well as morphogenesis of these foramina.

Original languageEnglish
Pages (from-to)388-396
Number of pages9
JournalDevelopment Genes and Evolution
Volume211
Issue number8-9
DOIs
Publication statusPublished - 2001

Fingerprint

Lampreys
Petromyzontiformes
Saccule and Utricle
Ear
canal
ears
epithelium
Epithelium
Morphogenesis
mutants
mice
Endolymphatic Duct
innervation
hair
hairs
morphogenesis
Inner Ear
Tubulin
Constriction
cells

Keywords

  • Ear development
  • Ear evolution
  • Ear innervation
  • Horizontal canal
  • Vestibular ganglion

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

Otx1 null mutant mice show partial segregation of sensory epithelia comparable to lamprey ears. / Fritzsch, B.; Signore, M.; Simeone, A.

In: Development Genes and Evolution, Vol. 211, No. 8-9, 2001, p. 388-396.

Research output: Contribution to journalArticle

Fritzsch, B. ; Signore, M. ; Simeone, A. / Otx1 null mutant mice show partial segregation of sensory epithelia comparable to lamprey ears. In: Development Genes and Evolution. 2001 ; Vol. 211, No. 8-9. pp. 388-396.
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