OTX2 homeodomain protein binds a DNA element necessary for interphotoreceptor retinoid binding protein gene expression

Nicoletta Bobola, Paola Briata, Cristina Ilengo, Nadia Rosatto, Cheryl Craft, Giorgio Corte, Roberto Ravazzolo

Research output: Contribution to journalArticle

Abstract

Transcription of the human interphotoreceptor retinoid binding protein (IRBP) gene is strictly tissue specific, being restricted to retinal photoreceptors and pinealocytes. We have previously demonstrated that a sequence named A element, in the IRBP promoter is essential for IRBP gene transcription in vivo. Here we demonstrate that the human homeodomain protein OTX2 is present in nuclear extracts of IRBP expressing cells and specifically interacts with the IRBP A promoter element in vitro. OTX2, as well as CRX, a homeodomain protein very similar to OTX2, activates the human IRBP promoter in co-transfection experiments.

Original languageEnglish
Pages (from-to)165-169
Number of pages5
JournalMechanisms of Development
Volume82
Issue number1-2
DOIs
Publication statusPublished - Apr 1 1999

Keywords

  • Bicoid
  • CRX
  • Developmen t
  • Dro sophila
  • Eye
  • Gel retardation
  • Gene
  • HeLa
  • Homeodomain
  • Human
  • Immunohistochemistry
  • Interphotoreceptor retinoid binding protein
  • IRBP
  • OTX2
  • Photoreceptors
  • Promoter
  • Retina
  • Retinoblastoma
  • Supershift
  • Transcriptional regulation
  • Transfection
  • Vertebrate
  • WERI- Rb1

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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