Ovarian cancer cytoreduction induces changes in T cell population subsets reducing immunosuppression

Chiara Napoletano, Filippo Bellati, Rachele Landi, Simona Pauselli, Claudia Marchetti, Valeria Visconti, Patrizio Sale, Marco Liberati, Aurelia Rughetti, Luigi Frati, Pierluigi Benedetti Panici, Marianna Nuti

Research output: Contribution to journalArticlepeer-review

Abstract

Surgery is the primary therapeutic strategy for most solid tumours; however, modern oncology has established that neoplasms are frequently systemic diseases. Being however a local treatment, the mechanisms through which surgery plays its systemic role remain unknown. We have investigated the influence of cytoreduction on the immune system of primary and recurrent ovarian cancer. All ovarian cancer patients show an increase in CD4+CD25+FOXP3+ circulating cells (CD4 Treg). CD4/CD8 ratio is increased in primary tumours, but not in recurrent neoplasms. Primary cytoreduction is able to increase circulating CD4 and CD8 effector cells and decrease CD4 naïve T cells. CD4+ Treg cells rapidly decreased after primary tumour debulking, while CD8+CD25+FOXP3+ (CD8 Treg) cells are not detectable in peripheral blood. Similar results on CD4 Treg were observed with chemical debulking in women subjected to neoadjuvant chemotherapy. CD4 and CD8 Treg cells are both present in neoplastic tissue. Interleukin (IL)-10 serum levels decrease after surgery, while no changes are observed in transforming growth factor-β1 and IL-6 levels. Surgically induced reduction of the immunosuppressive environment results in an increased capacity of CD8+ T cells to respond to the recall antigens. None of these changes was observed in patients previously subjected to chemotherapy or affected by recurrent disease. In conclusion, we demonstrate in ovarian cancer that primary debulking is associated with a reduction of circulating Treg and an increase in CD8 T-cell function. Debulking plays a beneficial systemic effect by reverting immunosuppression and restoring immunological fitness.

Original languageEnglish
Pages (from-to)2748-2759
Number of pages12
JournalJournal of Cellular and Molecular Medicine
Volume14
Issue number12
DOIs
Publication statusPublished - Dec 2010

Keywords

  • Interval debulking surgery
  • Ovarian cancer
  • Primary cytoreduction
  • Secondary cytoreduction
  • T cells

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine

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