Overcoming the hurdles for a reproducible generation of human functionally mature reprogrammed neurons

Vania Broccoli, Alicia Rubio, Stefano Taverna, Latefa Yekhlef

Research output: Contribution to journalArticle

Abstract

The advent of cell reprogramming technologies has widely disclosed the possibility to have direct access to human neurons for experimental and biomedical applications. Human pluripotent stem cells can be instructed in vitro to generate specific neuronal cell types as well as different glial cells. Moreover, new approaches of direct neuronal cell reprogramming can strongly accelerate the generation of different neuronal lineages. However, genetic heterogeneity, reprogramming fidelity, and time in culture of the starting cells can still significantly bias their differentiation efficiency and quality of the neuronal progenies. In addition, reprogrammed human neurons exhibit a very slow pace in gaining a full spectrum of functional properties including physiological levels of membrane excitability, sustained and prolonged action potential firing, mature synaptic currents and synaptic plasticity. This delay poses serious limitations for their significance as biological experimental model and screening platform. We will discuss new approaches of neuronal cell differentiation and reprogramming as well as methods to accelerate the maturation and functional activity of the converted human neurons.

Original languageEnglish
Pages (from-to)787-794
Number of pages8
JournalExperimental Biology and Medicine
Volume240
Issue number6
DOIs
Publication statusPublished - Jun 12 2015

Keywords

  • disease modeling
  • embryonic stem cells
  • induced pluripotent stem cells
  • neuronal activity
  • Neurons
  • synapses

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Overcoming the hurdles for a reproducible generation of human functionally mature reprogrammed neurons'. Together they form a unique fingerprint.

  • Cite this