Overexpression of a neural-specific Rho family GTPase, cRac1B, selectively induces enhanced neuritogenesis and neurite branching in primary neurons

Chiara Albertinazzi, Daniela Gilardelli, Simona Paris, Renato Longhi, Ivan De Curtis

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Rho family GTPases have been implicated in cytoskeletal reorganization during neuritogenesis. We have recently identified a new gene of this family, cRac1B, specifically expressed in the chicken developing nervous system. This GTPase was overexpressed in primary neurons to study the role of cRac1B in the development of the neuronal phenotype. Overexpression of cRac1B induced an increment in the number of neurites per neuron, and dramatically increased neurite branching, whereas overexpression of the highly related and ubiquitous cRac1A GTPase did not evidently affect neuronal morphology. Furthermore, expression of an inactive form of cRac1B strikingly inhibited neurite formation. The specificity of cRac1B action observed in neurons was not observed in fibroblasts, where both GTPases produced similar effects on cell morphology and actin organization, indicating the existence of a cell type-dependent specificity of cRac1B function. Molecular dissection of cRac1B function by analysis of the effects of chimetic cRac1A/cRacZ1B proteins showed that the COOH-terminal portion of cRac1B is essential to induce increased neuritogenesis and neurite branching. Considering the distinctive regulation of cRaclB expression during neural development, our data strongly support an important role of cRaclB during neuritogenesis, and they uncover new mechanisms underlying the functional specificity of distinct Rho family GTPases.

Original languageEnglish
Pages (from-to)815-825
Number of pages11
JournalJournal of Cell Biology
Volume142
Issue number3
DOIs
Publication statusPublished - Aug 10 1998

Fingerprint

rho GTP-Binding Proteins
Neurites
GTP Phosphohydrolases
Neurons
Nervous System
Dissection
Actins
Chickens
Fibroblasts
Phenotype
Genes
Proteins

Keywords

  • Actin
  • Cytoskeleton
  • Development
  • Neurites
  • Small GTPases

ASJC Scopus subject areas

  • Cell Biology

Cite this

Overexpression of a neural-specific Rho family GTPase, cRac1B, selectively induces enhanced neuritogenesis and neurite branching in primary neurons. / Albertinazzi, Chiara; Gilardelli, Daniela; Paris, Simona; Longhi, Renato; De Curtis, Ivan.

In: Journal of Cell Biology, Vol. 142, No. 3, 10.08.1998, p. 815-825.

Research output: Contribution to journalArticle

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