Overexpression of ADAR1 into the cytoplasm correlates with a better prognosis of patients with oral squamous cells carcinoma

Vito Carlo Alberto Caponio, Giuseppe Troiano, Gerardo Botti, Maria Carmela Pedicillo, Lucio Lo Russo, Filiberto Mastrangelo, Domenico Ciavarella, Nunzia Simona Losito, Gabriella Aquino, Riccardo Nocini, Rossella Santoro, Angela Santoro, Lorenzo Lo Muzio, Giuseppe Pannone

Research output: Contribution to journalArticle

Abstract

Background: ADAR1 is an enzymatic protein, which catalyzes a RNA editing reaction by converting Adenosine to Inosine, and its expression has been found to be dysregulated in many cancer types. The aim of this study was to analyze the expression of ADAR1 in oral squamous cells carcinoma. Methods: In order to analyze the ADAR1 mRNA expression, data from The Cancer Genome Atlas (TCGA) database were downloaded and analyzed. In addition, immunohistochemistry analysis was performed on an institutional database including 46 samples of oral squamous cell carcinoma in a tissue microarray (TMA). Results: No statistically significant correlation linked the mRNA ADAR1 expression to any clinic-pathological variables in the TCGA database. Immunohistochemistry analysis of ADAR1 showed different expressions between normal mucosa and tumor tissue. Focusing on the subcellular localization, the nuclear expression of ADAR1 correlated with higher grading of differentiation (ρ = 0.442; P-value = 0.002); the general expression of ADAR1 either in cytoplasm or in nuclei, correlated with the Gender of patients (Cytoplasm expression: ρ = −0.295; P-value = 0.049; while for nuclear expression: ρ = +0.374; P = 0.011); cytosol expression resulted to be an independent protective prognostic factor (HR = 0.047; C.I. 95% 0.007-0.321; P-value = 0.002). Conclusion: Higher expression of ADAR1 into the cytoplasm resulted to be an independent prognostic factor. In order to understand ADAR1 role in cancer, further studies should be performed, in bigger cohort and under a bio-molecular point of view.

Original languageEnglish
Pages (from-to)108-114
Number of pages7
JournalJournal of Oral Pathology and Medicine
Volume48
Issue number2
DOIs
Publication statusPublished - Feb 1 2019

Fingerprint

Squamous Cell Carcinoma
Cytoplasm
Atlases
Databases
Neoplasms
Immunohistochemistry
Genome
RNA Editing
Inosine
Messenger RNA
Cytosol
Adenosine
Mucous Membrane
Proteins

Keywords

  • ADAR
  • head neck
  • oral cancer
  • oral squamous cell carcinoma
  • prognosis
  • RNA editing
  • RNA-binding protein
  • survival
  • TCGA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

Cite this

Overexpression of ADAR1 into the cytoplasm correlates with a better prognosis of patients with oral squamous cells carcinoma. / Caponio, Vito Carlo Alberto; Troiano, Giuseppe; Botti, Gerardo; Pedicillo, Maria Carmela; Lo Russo, Lucio; Mastrangelo, Filiberto; Ciavarella, Domenico; Losito, Nunzia Simona; Aquino, Gabriella; Nocini, Riccardo; Santoro, Rossella; Santoro, Angela; Lo Muzio, Lorenzo; Pannone, Giuseppe.

In: Journal of Oral Pathology and Medicine, Vol. 48, No. 2, 01.02.2019, p. 108-114.

Research output: Contribution to journalArticle

Caponio, VCA, Troiano, G, Botti, G, Pedicillo, MC, Lo Russo, L, Mastrangelo, F, Ciavarella, D, Losito, NS, Aquino, G, Nocini, R, Santoro, R, Santoro, A, Lo Muzio, L & Pannone, G 2019, 'Overexpression of ADAR1 into the cytoplasm correlates with a better prognosis of patients with oral squamous cells carcinoma', Journal of Oral Pathology and Medicine, vol. 48, no. 2, pp. 108-114. https://doi.org/10.1111/jop.12808
Caponio, Vito Carlo Alberto ; Troiano, Giuseppe ; Botti, Gerardo ; Pedicillo, Maria Carmela ; Lo Russo, Lucio ; Mastrangelo, Filiberto ; Ciavarella, Domenico ; Losito, Nunzia Simona ; Aquino, Gabriella ; Nocini, Riccardo ; Santoro, Rossella ; Santoro, Angela ; Lo Muzio, Lorenzo ; Pannone, Giuseppe. / Overexpression of ADAR1 into the cytoplasm correlates with a better prognosis of patients with oral squamous cells carcinoma. In: Journal of Oral Pathology and Medicine. 2019 ; Vol. 48, No. 2. pp. 108-114.
@article{ff87c95c5e9745f8b34497d8143e9715,
title = "Overexpression of ADAR1 into the cytoplasm correlates with a better prognosis of patients with oral squamous cells carcinoma",
abstract = "Background: ADAR1 is an enzymatic protein, which catalyzes a RNA editing reaction by converting Adenosine to Inosine, and its expression has been found to be dysregulated in many cancer types. The aim of this study was to analyze the expression of ADAR1 in oral squamous cells carcinoma. Methods: In order to analyze the ADAR1 mRNA expression, data from The Cancer Genome Atlas (TCGA) database were downloaded and analyzed. In addition, immunohistochemistry analysis was performed on an institutional database including 46 samples of oral squamous cell carcinoma in a tissue microarray (TMA). Results: No statistically significant correlation linked the mRNA ADAR1 expression to any clinic-pathological variables in the TCGA database. Immunohistochemistry analysis of ADAR1 showed different expressions between normal mucosa and tumor tissue. Focusing on the subcellular localization, the nuclear expression of ADAR1 correlated with higher grading of differentiation (ρ = 0.442; P-value = 0.002); the general expression of ADAR1 either in cytoplasm or in nuclei, correlated with the Gender of patients (Cytoplasm expression: ρ = −0.295; P-value = 0.049; while for nuclear expression: ρ = +0.374; P = 0.011); cytosol expression resulted to be an independent protective prognostic factor (HR = 0.047; C.I. 95{\%} 0.007-0.321; P-value = 0.002). Conclusion: Higher expression of ADAR1 into the cytoplasm resulted to be an independent prognostic factor. In order to understand ADAR1 role in cancer, further studies should be performed, in bigger cohort and under a bio-molecular point of view.",
keywords = "ADAR, head neck, oral cancer, oral squamous cell carcinoma, prognosis, RNA editing, RNA-binding protein, survival, TCGA",
author = "Caponio, {Vito Carlo Alberto} and Giuseppe Troiano and Gerardo Botti and Pedicillo, {Maria Carmela} and {Lo Russo}, Lucio and Filiberto Mastrangelo and Domenico Ciavarella and Losito, {Nunzia Simona} and Gabriella Aquino and Riccardo Nocini and Rossella Santoro and Angela Santoro and {Lo Muzio}, Lorenzo and Giuseppe Pannone",
year = "2019",
month = "2",
day = "1",
doi = "10.1111/jop.12808",
language = "English",
volume = "48",
pages = "108--114",
journal = "Journal of Oral Pathology and Medicine",
issn = "0904-2512",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Overexpression of ADAR1 into the cytoplasm correlates with a better prognosis of patients with oral squamous cells carcinoma

AU - Caponio, Vito Carlo Alberto

AU - Troiano, Giuseppe

AU - Botti, Gerardo

AU - Pedicillo, Maria Carmela

AU - Lo Russo, Lucio

AU - Mastrangelo, Filiberto

AU - Ciavarella, Domenico

AU - Losito, Nunzia Simona

AU - Aquino, Gabriella

AU - Nocini, Riccardo

AU - Santoro, Rossella

AU - Santoro, Angela

AU - Lo Muzio, Lorenzo

AU - Pannone, Giuseppe

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Background: ADAR1 is an enzymatic protein, which catalyzes a RNA editing reaction by converting Adenosine to Inosine, and its expression has been found to be dysregulated in many cancer types. The aim of this study was to analyze the expression of ADAR1 in oral squamous cells carcinoma. Methods: In order to analyze the ADAR1 mRNA expression, data from The Cancer Genome Atlas (TCGA) database were downloaded and analyzed. In addition, immunohistochemistry analysis was performed on an institutional database including 46 samples of oral squamous cell carcinoma in a tissue microarray (TMA). Results: No statistically significant correlation linked the mRNA ADAR1 expression to any clinic-pathological variables in the TCGA database. Immunohistochemistry analysis of ADAR1 showed different expressions between normal mucosa and tumor tissue. Focusing on the subcellular localization, the nuclear expression of ADAR1 correlated with higher grading of differentiation (ρ = 0.442; P-value = 0.002); the general expression of ADAR1 either in cytoplasm or in nuclei, correlated with the Gender of patients (Cytoplasm expression: ρ = −0.295; P-value = 0.049; while for nuclear expression: ρ = +0.374; P = 0.011); cytosol expression resulted to be an independent protective prognostic factor (HR = 0.047; C.I. 95% 0.007-0.321; P-value = 0.002). Conclusion: Higher expression of ADAR1 into the cytoplasm resulted to be an independent prognostic factor. In order to understand ADAR1 role in cancer, further studies should be performed, in bigger cohort and under a bio-molecular point of view.

AB - Background: ADAR1 is an enzymatic protein, which catalyzes a RNA editing reaction by converting Adenosine to Inosine, and its expression has been found to be dysregulated in many cancer types. The aim of this study was to analyze the expression of ADAR1 in oral squamous cells carcinoma. Methods: In order to analyze the ADAR1 mRNA expression, data from The Cancer Genome Atlas (TCGA) database were downloaded and analyzed. In addition, immunohistochemistry analysis was performed on an institutional database including 46 samples of oral squamous cell carcinoma in a tissue microarray (TMA). Results: No statistically significant correlation linked the mRNA ADAR1 expression to any clinic-pathological variables in the TCGA database. Immunohistochemistry analysis of ADAR1 showed different expressions between normal mucosa and tumor tissue. Focusing on the subcellular localization, the nuclear expression of ADAR1 correlated with higher grading of differentiation (ρ = 0.442; P-value = 0.002); the general expression of ADAR1 either in cytoplasm or in nuclei, correlated with the Gender of patients (Cytoplasm expression: ρ = −0.295; P-value = 0.049; while for nuclear expression: ρ = +0.374; P = 0.011); cytosol expression resulted to be an independent protective prognostic factor (HR = 0.047; C.I. 95% 0.007-0.321; P-value = 0.002). Conclusion: Higher expression of ADAR1 into the cytoplasm resulted to be an independent prognostic factor. In order to understand ADAR1 role in cancer, further studies should be performed, in bigger cohort and under a bio-molecular point of view.

KW - ADAR

KW - head neck

KW - oral cancer

KW - oral squamous cell carcinoma

KW - prognosis

KW - RNA editing

KW - RNA-binding protein

KW - survival

KW - TCGA

UR - http://www.scopus.com/inward/record.url?scp=85058670211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85058670211&partnerID=8YFLogxK

U2 - 10.1111/jop.12808

DO - 10.1111/jop.12808

M3 - Article

VL - 48

SP - 108

EP - 114

JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

SN - 0904-2512

IS - 2

ER -