Overexpression of BCL-XL underlies the molecular basis for resistance to staurosporine-induced apoptosis in PC-3 cells

Xiao Ying Li, Michela Marani, Roberta Mannucci, Berma Kinsey, Francesca Andriani, Ildo Nicoletti, Larry Denner, Marco Marcelli

Research output: Contribution to journalArticle

Abstract

We have reported previously that among human prostate cancer cell lines LNCaP but not PC-3 cells undergo apoptosis after treatment with the protein kinase inhibitor staurosporine (STS). We have now further investigated this model to uncover the molecular mechanism causing resistance to STS-induced apoptosis in PC-3 cells. S-100 lysates of both cell lines showed biochemical changes typical of apoptosis after the addition of cytochrome c and dATP, suggesting that the postmitochondrial phase of apoptosis was intact. Upon addition of STS, the proapoptotic molecules Bax and Bad became predominantly mitochondrial in both cell lines. This, in turn, was followed by loss of mitochondrial transmembrane potential, translocation of cytochrome c to the cytosol, activation of caspase-9, -3, and -7, and cleavage of the apoptotic targets, DNA fragmentation factor and poly(ADP-ribose) polymerase, in LNCaP but not in PC-3 cells. Components of the mitochondrial permeability transition pore, adenine nucleotide transporter and voltage- dependent anion channel, were normally expressed in the correct subcellular fraction of both cell lines. Overexpression of the proapoptotic proteins Bax and Bad, fused to a green fluorescent protein but not of green fluorescent protein alone, induced apoptosis in >80% of PC-3 cells. These experiments suggested that a factor protecting the mitochondria of PC-3 cells mediates resistance to STS-induced apoptosis. A wide search among the antiapoptotic Bcl-2 family members was performed, and Bcl-XL was found to be overexpresseÃd in PC-3 cells. Experiments down-regulating Bcl-XL expression by using the tyrosine kinase inhibitor genistein, sodium butyrate, or an antisense Bcl-XL oligonucleotide restored sensitivity to apoptosis in PC-3 cells. Thus, Bcl-XL overexpression is one of the mediators of resistance to STS-induced apoptosis in the prostate cancer cell line PC-3.

Original languageEnglish
Pages (from-to)1699-1706
Number of pages8
JournalCancer Research
Volume61
Issue number4
Publication statusPublished - Feb 15 2001

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint Dive into the research topics of 'Overexpression of BCL-XL underlies the molecular basis for resistance to staurosporine-induced apoptosis in PC-3 cells'. Together they form a unique fingerprint.

  • Cite this

    Li, X. Y., Marani, M., Mannucci, R., Kinsey, B., Andriani, F., Nicoletti, I., Denner, L., & Marcelli, M. (2001). Overexpression of BCL-XL underlies the molecular basis for resistance to staurosporine-induced apoptosis in PC-3 cells. Cancer Research, 61(4), 1699-1706.