Overexpression of ELAV-like Protein HuR is Associated with Increased COX-2 Expression in Atrophy, High-grade Prostatic Intraepithelial Neoplasia, and Incidental Prostate Cancer in Cystoprostatectomies

Francesca Barbisan, Roberta Mazzucchelli, Alfredo Santinelli, Antonio Lopez-Beltran, Liang Cheng, Marina Scarpelli, Francesco Montorsi, Rodolfo Montironi

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Background: The human ELAV-like protein HuR regulates the stability of several mRNA targets, including that of cyclooygenase-2 (COX-2). Their expression in prostatic carcinogenesis is uncertain. Objective: To analyze HuR and COX-2 expression in cystoprostatectomies (CyPs) with incidental prostate cancer and compare their expression with those in radical prostatectomies (RPs) with clinically detected cancer. Design, setting, and participants: HuR and COX-2 were immunohistochemically evaluated in normal-looking epithelium (NEp), atrophy, high-grade prostatic intraepithelial neoplasia (HGPIN), and prostate carcinoma (PCa) in 20 CyPs and 20 RPs, both types of specimens with pT2a Gleason score 6 PCa. Measurements: At least 1000 cells were counted in contiguous 400X microscopic fields in each case, separately for NEp, atrophy, HGPIN, and PCa. Results and limitations: There was an increase in the percentage of secretory cells with cytoplasmic HuR staining from NEp to atrophy, HGPIN, and PCa. The mean percentages in NEp, atrophy, and HGPIN adjacent to PCa were greater than away from cancer, both in the CyP and RPs. There was a trend towards a reduced nuclear HuR expression in atrophy, HGPIN, and PCa, compared to NEp. COX-2 staining was seen in the cytoplasm of the basal and secretory cells. There was a reduction in the mean proportion of positive basal cells and progressive increase in the percentage of positive secretory cells from atrophy to HGPIN and PCa, compared to NEp. Cytoplasmic HuR overexpression was correlated with COX-2 expression. There was no difference in HuR and COX-2 expression between cancers with tumour volume 0.5 ccm. The limitations of this study were the small number of cases investigated and lack of a control group without cancer. Conclusions: The secretory cells showed shift in HuR staining from nuclear in NEp to cytoplasmic in PCa. This is associated with a parallel shift in COX-2 expression from basal to secretory cells.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalEuropean Urology
Volume56
Issue number1
DOIs
Publication statusPublished - Jul 2009

Fingerprint

ELAV Proteins
Prostatic Intraepithelial Neoplasia
Atrophy
Prostate
Prostatic Neoplasms
Epithelium
Carcinoma
Prostatectomy
Staining and Labeling
Neoplasms
Neoplasm Grading
RNA Stability
Tumor Burden
Carcinogenesis
Cytoplasm

Keywords

  • Atrophy
  • COX-2
  • Cystoprostatectomy
  • High-grade prostatic intraepithelial neoplasia
  • HuR
  • Incidental cancer
  • Prostate cancer

ASJC Scopus subject areas

  • Urology

Cite this

Overexpression of ELAV-like Protein HuR is Associated with Increased COX-2 Expression in Atrophy, High-grade Prostatic Intraepithelial Neoplasia, and Incidental Prostate Cancer in Cystoprostatectomies. / Barbisan, Francesca; Mazzucchelli, Roberta; Santinelli, Alfredo; Lopez-Beltran, Antonio; Cheng, Liang; Scarpelli, Marina; Montorsi, Francesco; Montironi, Rodolfo.

In: European Urology, Vol. 56, No. 1, 07.2009, p. 105-112.

Research output: Contribution to journalArticle

Barbisan, Francesca ; Mazzucchelli, Roberta ; Santinelli, Alfredo ; Lopez-Beltran, Antonio ; Cheng, Liang ; Scarpelli, Marina ; Montorsi, Francesco ; Montironi, Rodolfo. / Overexpression of ELAV-like Protein HuR is Associated with Increased COX-2 Expression in Atrophy, High-grade Prostatic Intraepithelial Neoplasia, and Incidental Prostate Cancer in Cystoprostatectomies. In: European Urology. 2009 ; Vol. 56, No. 1. pp. 105-112.
@article{576fa73b7b9e45c9bdf9b6f995e7b093,
title = "Overexpression of ELAV-like Protein HuR is Associated with Increased COX-2 Expression in Atrophy, High-grade Prostatic Intraepithelial Neoplasia, and Incidental Prostate Cancer in Cystoprostatectomies",
abstract = "Background: The human ELAV-like protein HuR regulates the stability of several mRNA targets, including that of cyclooygenase-2 (COX-2). Their expression in prostatic carcinogenesis is uncertain. Objective: To analyze HuR and COX-2 expression in cystoprostatectomies (CyPs) with incidental prostate cancer and compare their expression with those in radical prostatectomies (RPs) with clinically detected cancer. Design, setting, and participants: HuR and COX-2 were immunohistochemically evaluated in normal-looking epithelium (NEp), atrophy, high-grade prostatic intraepithelial neoplasia (HGPIN), and prostate carcinoma (PCa) in 20 CyPs and 20 RPs, both types of specimens with pT2a Gleason score 6 PCa. Measurements: At least 1000 cells were counted in contiguous 400X microscopic fields in each case, separately for NEp, atrophy, HGPIN, and PCa. Results and limitations: There was an increase in the percentage of secretory cells with cytoplasmic HuR staining from NEp to atrophy, HGPIN, and PCa. The mean percentages in NEp, atrophy, and HGPIN adjacent to PCa were greater than away from cancer, both in the CyP and RPs. There was a trend towards a reduced nuclear HuR expression in atrophy, HGPIN, and PCa, compared to NEp. COX-2 staining was seen in the cytoplasm of the basal and secretory cells. There was a reduction in the mean proportion of positive basal cells and progressive increase in the percentage of positive secretory cells from atrophy to HGPIN and PCa, compared to NEp. Cytoplasmic HuR overexpression was correlated with COX-2 expression. There was no difference in HuR and COX-2 expression between cancers with tumour volume 0.5 ccm. The limitations of this study were the small number of cases investigated and lack of a control group without cancer. Conclusions: The secretory cells showed shift in HuR staining from nuclear in NEp to cytoplasmic in PCa. This is associated with a parallel shift in COX-2 expression from basal to secretory cells.",
keywords = "Atrophy, COX-2, Cystoprostatectomy, High-grade prostatic intraepithelial neoplasia, HuR, Incidental cancer, Prostate cancer",
author = "Francesca Barbisan and Roberta Mazzucchelli and Alfredo Santinelli and Antonio Lopez-Beltran and Liang Cheng and Marina Scarpelli and Francesco Montorsi and Rodolfo Montironi",
year = "2009",
month = "7",
doi = "10.1016/j.eururo.2008.04.043",
language = "English",
volume = "56",
pages = "105--112",
journal = "European Urology",
issn = "0302-2838",
publisher = "Elsevier B.V.",
number = "1",

}

TY - JOUR

T1 - Overexpression of ELAV-like Protein HuR is Associated with Increased COX-2 Expression in Atrophy, High-grade Prostatic Intraepithelial Neoplasia, and Incidental Prostate Cancer in Cystoprostatectomies

AU - Barbisan, Francesca

AU - Mazzucchelli, Roberta

AU - Santinelli, Alfredo

AU - Lopez-Beltran, Antonio

AU - Cheng, Liang

AU - Scarpelli, Marina

AU - Montorsi, Francesco

AU - Montironi, Rodolfo

PY - 2009/7

Y1 - 2009/7

N2 - Background: The human ELAV-like protein HuR regulates the stability of several mRNA targets, including that of cyclooygenase-2 (COX-2). Their expression in prostatic carcinogenesis is uncertain. Objective: To analyze HuR and COX-2 expression in cystoprostatectomies (CyPs) with incidental prostate cancer and compare their expression with those in radical prostatectomies (RPs) with clinically detected cancer. Design, setting, and participants: HuR and COX-2 were immunohistochemically evaluated in normal-looking epithelium (NEp), atrophy, high-grade prostatic intraepithelial neoplasia (HGPIN), and prostate carcinoma (PCa) in 20 CyPs and 20 RPs, both types of specimens with pT2a Gleason score 6 PCa. Measurements: At least 1000 cells were counted in contiguous 400X microscopic fields in each case, separately for NEp, atrophy, HGPIN, and PCa. Results and limitations: There was an increase in the percentage of secretory cells with cytoplasmic HuR staining from NEp to atrophy, HGPIN, and PCa. The mean percentages in NEp, atrophy, and HGPIN adjacent to PCa were greater than away from cancer, both in the CyP and RPs. There was a trend towards a reduced nuclear HuR expression in atrophy, HGPIN, and PCa, compared to NEp. COX-2 staining was seen in the cytoplasm of the basal and secretory cells. There was a reduction in the mean proportion of positive basal cells and progressive increase in the percentage of positive secretory cells from atrophy to HGPIN and PCa, compared to NEp. Cytoplasmic HuR overexpression was correlated with COX-2 expression. There was no difference in HuR and COX-2 expression between cancers with tumour volume 0.5 ccm. The limitations of this study were the small number of cases investigated and lack of a control group without cancer. Conclusions: The secretory cells showed shift in HuR staining from nuclear in NEp to cytoplasmic in PCa. This is associated with a parallel shift in COX-2 expression from basal to secretory cells.

AB - Background: The human ELAV-like protein HuR regulates the stability of several mRNA targets, including that of cyclooygenase-2 (COX-2). Their expression in prostatic carcinogenesis is uncertain. Objective: To analyze HuR and COX-2 expression in cystoprostatectomies (CyPs) with incidental prostate cancer and compare their expression with those in radical prostatectomies (RPs) with clinically detected cancer. Design, setting, and participants: HuR and COX-2 were immunohistochemically evaluated in normal-looking epithelium (NEp), atrophy, high-grade prostatic intraepithelial neoplasia (HGPIN), and prostate carcinoma (PCa) in 20 CyPs and 20 RPs, both types of specimens with pT2a Gleason score 6 PCa. Measurements: At least 1000 cells were counted in contiguous 400X microscopic fields in each case, separately for NEp, atrophy, HGPIN, and PCa. Results and limitations: There was an increase in the percentage of secretory cells with cytoplasmic HuR staining from NEp to atrophy, HGPIN, and PCa. The mean percentages in NEp, atrophy, and HGPIN adjacent to PCa were greater than away from cancer, both in the CyP and RPs. There was a trend towards a reduced nuclear HuR expression in atrophy, HGPIN, and PCa, compared to NEp. COX-2 staining was seen in the cytoplasm of the basal and secretory cells. There was a reduction in the mean proportion of positive basal cells and progressive increase in the percentage of positive secretory cells from atrophy to HGPIN and PCa, compared to NEp. Cytoplasmic HuR overexpression was correlated with COX-2 expression. There was no difference in HuR and COX-2 expression between cancers with tumour volume 0.5 ccm. The limitations of this study were the small number of cases investigated and lack of a control group without cancer. Conclusions: The secretory cells showed shift in HuR staining from nuclear in NEp to cytoplasmic in PCa. This is associated with a parallel shift in COX-2 expression from basal to secretory cells.

KW - Atrophy

KW - COX-2

KW - Cystoprostatectomy

KW - High-grade prostatic intraepithelial neoplasia

KW - HuR

KW - Incidental cancer

KW - Prostate cancer

UR - http://www.scopus.com/inward/record.url?scp=67349194017&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349194017&partnerID=8YFLogxK

U2 - 10.1016/j.eururo.2008.04.043

DO - 10.1016/j.eururo.2008.04.043

M3 - Article

C2 - 18468781

AN - SCOPUS:67349194017

VL - 56

SP - 105

EP - 112

JO - European Urology

JF - European Urology

SN - 0302-2838

IS - 1

ER -