Overexpression of h-prune in breast cancer is correlated with advanced disease status

Massimo Zollo, Alessandra Andrè, Antonio Cossu, Maria C. Sini, Anna D'Angelo, Natascia Marino, Mario Budroni, Francesco Tanda, Gianluigi Arrigoni, Giuseppe Palmieri

Research output: Contribution to journalArticle

Abstract

Purpose: The h-prune gene is involved in cellular motility and metastasis formation in breast cancer through interacting with the nm23-H1 protein. The aim of this study was to better define the clinical and pathologic role of h-prune in breast cancer patients. Experimental Design: Using immunohistochemistry, we assessed h-prune and nm23-H1 protein expression in two series of breast cancer patients: (i) in 2,109 cases with pathologic reports on primary tumors and (ii) in 412 cases with detailed clinical information. To assess the role of DNA amplification in gene activation, the h-prune copy number was evaluated by fluorescence in situ hybridization analysis in 1,016 breast cancer cases. Results: In the patients tested (n = 2,463), 1,340 (54%) had an increased level of h-prune expression; a positive immunostaining for nm23-H1 was observed in 615 of 2,061 (30%) cases. Overexpression of h-prune was associated with multiple gene copy number at chromosome 1q21.3 in a very limited fraction of cases (68 of 1,016; 6.7%), strongly indicating that alternative pathways induce h-prune activation in breast cancer. Multivariate Cox regression analysis showed that neither h-prune overexpression nor decreased nm23-H1 immunostaining is independent prognostic factors. However, a significant association of h-prune overexpression with either advanced lymph node status (P = 0.017) or presence of distant metastases (P = 0.029) was observed. Conclusions: Although not significantly correlated with overall survival, positive h-prune immunostaining identifies subsets of breast cancer patients with higher tumor aggressiveness. Further investigations using larger collections of advanced breast cancer patients are required for assessing the predictive role of h-prune in breast cancer.

Original languageEnglish
Pages (from-to)199-205
Number of pages7
JournalClinical Cancer Research
Volume11
Issue number1
Publication statusPublished - Jan 1 2005

Fingerprint

Breast Neoplasms
Neoplasm Metastasis
Gene Dosage
Fluorescence In Situ Hybridization
Transcriptional Activation
Neoplasms
Proteins
Research Design
Chromosomes
Lymph Nodes
Immunohistochemistry
Regression Analysis
Survival
DNA
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Zollo, M., Andrè, A., Cossu, A., Sini, M. C., D'Angelo, A., Marino, N., ... Palmieri, G. (2005). Overexpression of h-prune in breast cancer is correlated with advanced disease status. Clinical Cancer Research, 11(1), 199-205.

Overexpression of h-prune in breast cancer is correlated with advanced disease status. / Zollo, Massimo; Andrè, Alessandra; Cossu, Antonio; Sini, Maria C.; D'Angelo, Anna; Marino, Natascia; Budroni, Mario; Tanda, Francesco; Arrigoni, Gianluigi; Palmieri, Giuseppe.

In: Clinical Cancer Research, Vol. 11, No. 1, 01.01.2005, p. 199-205.

Research output: Contribution to journalArticle

Zollo, M, Andrè, A, Cossu, A, Sini, MC, D'Angelo, A, Marino, N, Budroni, M, Tanda, F, Arrigoni, G & Palmieri, G 2005, 'Overexpression of h-prune in breast cancer is correlated with advanced disease status', Clinical Cancer Research, vol. 11, no. 1, pp. 199-205.
Zollo M, Andrè A, Cossu A, Sini MC, D'Angelo A, Marino N et al. Overexpression of h-prune in breast cancer is correlated with advanced disease status. Clinical Cancer Research. 2005 Jan 1;11(1):199-205.
Zollo, Massimo ; Andrè, Alessandra ; Cossu, Antonio ; Sini, Maria C. ; D'Angelo, Anna ; Marino, Natascia ; Budroni, Mario ; Tanda, Francesco ; Arrigoni, Gianluigi ; Palmieri, Giuseppe. / Overexpression of h-prune in breast cancer is correlated with advanced disease status. In: Clinical Cancer Research. 2005 ; Vol. 11, No. 1. pp. 199-205.
@article{20a1b7b40c0d47048d0ae4b121e399ad,
title = "Overexpression of h-prune in breast cancer is correlated with advanced disease status",
abstract = "Purpose: The h-prune gene is involved in cellular motility and metastasis formation in breast cancer through interacting with the nm23-H1 protein. The aim of this study was to better define the clinical and pathologic role of h-prune in breast cancer patients. Experimental Design: Using immunohistochemistry, we assessed h-prune and nm23-H1 protein expression in two series of breast cancer patients: (i) in 2,109 cases with pathologic reports on primary tumors and (ii) in 412 cases with detailed clinical information. To assess the role of DNA amplification in gene activation, the h-prune copy number was evaluated by fluorescence in situ hybridization analysis in 1,016 breast cancer cases. Results: In the patients tested (n = 2,463), 1,340 (54{\%}) had an increased level of h-prune expression; a positive immunostaining for nm23-H1 was observed in 615 of 2,061 (30{\%}) cases. Overexpression of h-prune was associated with multiple gene copy number at chromosome 1q21.3 in a very limited fraction of cases (68 of 1,016; 6.7{\%}), strongly indicating that alternative pathways induce h-prune activation in breast cancer. Multivariate Cox regression analysis showed that neither h-prune overexpression nor decreased nm23-H1 immunostaining is independent prognostic factors. However, a significant association of h-prune overexpression with either advanced lymph node status (P = 0.017) or presence of distant metastases (P = 0.029) was observed. Conclusions: Although not significantly correlated with overall survival, positive h-prune immunostaining identifies subsets of breast cancer patients with higher tumor aggressiveness. Further investigations using larger collections of advanced breast cancer patients are required for assessing the predictive role of h-prune in breast cancer.",
author = "Massimo Zollo and Alessandra Andr{\`e} and Antonio Cossu and Sini, {Maria C.} and Anna D'Angelo and Natascia Marino and Mario Budroni and Francesco Tanda and Gianluigi Arrigoni and Giuseppe Palmieri",
year = "2005",
month = "1",
day = "1",
language = "English",
volume = "11",
pages = "199--205",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "1",

}

TY - JOUR

T1 - Overexpression of h-prune in breast cancer is correlated with advanced disease status

AU - Zollo, Massimo

AU - Andrè, Alessandra

AU - Cossu, Antonio

AU - Sini, Maria C.

AU - D'Angelo, Anna

AU - Marino, Natascia

AU - Budroni, Mario

AU - Tanda, Francesco

AU - Arrigoni, Gianluigi

AU - Palmieri, Giuseppe

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Purpose: The h-prune gene is involved in cellular motility and metastasis formation in breast cancer through interacting with the nm23-H1 protein. The aim of this study was to better define the clinical and pathologic role of h-prune in breast cancer patients. Experimental Design: Using immunohistochemistry, we assessed h-prune and nm23-H1 protein expression in two series of breast cancer patients: (i) in 2,109 cases with pathologic reports on primary tumors and (ii) in 412 cases with detailed clinical information. To assess the role of DNA amplification in gene activation, the h-prune copy number was evaluated by fluorescence in situ hybridization analysis in 1,016 breast cancer cases. Results: In the patients tested (n = 2,463), 1,340 (54%) had an increased level of h-prune expression; a positive immunostaining for nm23-H1 was observed in 615 of 2,061 (30%) cases. Overexpression of h-prune was associated with multiple gene copy number at chromosome 1q21.3 in a very limited fraction of cases (68 of 1,016; 6.7%), strongly indicating that alternative pathways induce h-prune activation in breast cancer. Multivariate Cox regression analysis showed that neither h-prune overexpression nor decreased nm23-H1 immunostaining is independent prognostic factors. However, a significant association of h-prune overexpression with either advanced lymph node status (P = 0.017) or presence of distant metastases (P = 0.029) was observed. Conclusions: Although not significantly correlated with overall survival, positive h-prune immunostaining identifies subsets of breast cancer patients with higher tumor aggressiveness. Further investigations using larger collections of advanced breast cancer patients are required for assessing the predictive role of h-prune in breast cancer.

AB - Purpose: The h-prune gene is involved in cellular motility and metastasis formation in breast cancer through interacting with the nm23-H1 protein. The aim of this study was to better define the clinical and pathologic role of h-prune in breast cancer patients. Experimental Design: Using immunohistochemistry, we assessed h-prune and nm23-H1 protein expression in two series of breast cancer patients: (i) in 2,109 cases with pathologic reports on primary tumors and (ii) in 412 cases with detailed clinical information. To assess the role of DNA amplification in gene activation, the h-prune copy number was evaluated by fluorescence in situ hybridization analysis in 1,016 breast cancer cases. Results: In the patients tested (n = 2,463), 1,340 (54%) had an increased level of h-prune expression; a positive immunostaining for nm23-H1 was observed in 615 of 2,061 (30%) cases. Overexpression of h-prune was associated with multiple gene copy number at chromosome 1q21.3 in a very limited fraction of cases (68 of 1,016; 6.7%), strongly indicating that alternative pathways induce h-prune activation in breast cancer. Multivariate Cox regression analysis showed that neither h-prune overexpression nor decreased nm23-H1 immunostaining is independent prognostic factors. However, a significant association of h-prune overexpression with either advanced lymph node status (P = 0.017) or presence of distant metastases (P = 0.029) was observed. Conclusions: Although not significantly correlated with overall survival, positive h-prune immunostaining identifies subsets of breast cancer patients with higher tumor aggressiveness. Further investigations using larger collections of advanced breast cancer patients are required for assessing the predictive role of h-prune in breast cancer.

UR - http://www.scopus.com/inward/record.url?scp=19944429294&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19944429294&partnerID=8YFLogxK

M3 - Article

C2 - 15671547

AN - SCOPUS:19944429294

VL - 11

SP - 199

EP - 205

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

IS - 1

ER -