Abstract
Five human glioblastoma cell lines were analyzed for oncogene activation with a panel of probes. Abnormal expression of the epidermal growth factor receptor (EGFr) gene was detected in four of five lines; N-ras oncogene overexpression was found in all five cell lines. These results were subsequently confirmed with fresh brain tumor and nonneoplastic brain tissue biopsy samples; increased expression of the N-ras proto-oncogene was observed in five of five glioblastomas, all of which also showed EGFr gene overex-pression, but not in well-differentiated gliomas or in nonneoplastic brain tis-sue specimens. No significant differ-ences in Ha-ras and Ki-ras expression were observed. Preliminary histochem-ical observations showed that intra-cellular levels of transforming growth factor α, a putative biochemical link between these two oncogenes, were significantly higher in glioblastoma cells than in controls.
| Original language | English |
|---|---|
| Pages (from-to) | 63-67 |
| Number of pages | 5 |
| Journal | Journal of the National Cancer Institute |
| Volume | 81 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1989 |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
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Overexpression of n-ras oncogene and epidermal growth factor receptor gene in human glioblastomas. / Gerosa, Massimo A.; Colombatti, Marco; Tridente, Giuseppe; Talarico, Daniela; Fognani, Caterina; Raimondi, Elena; Carli, Luigi De; Valle, Giuliano Della.
In: Journal of the National Cancer Institute, Vol. 81, No. 1, 1989, p. 63-67.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Overexpression of n-ras oncogene and epidermal growth factor receptor gene in human glioblastomas
AU - Gerosa, Massimo A.
AU - Colombatti, Marco
AU - Tridente, Giuseppe
AU - Talarico, Daniela
AU - Fognani, Caterina
AU - Raimondi, Elena
AU - Carli, Luigi De
AU - Valle, Giuliano Della
PY - 1989
Y1 - 1989
N2 - Five human glioblastoma cell lines were analyzed for oncogene activation with a panel of probes. Abnormal expression of the epidermal growth factor receptor (EGFr) gene was detected in four of five lines; N-ras oncogene overexpression was found in all five cell lines. These results were subsequently confirmed with fresh brain tumor and nonneoplastic brain tissue biopsy samples; increased expression of the N-ras proto-oncogene was observed in five of five glioblastomas, all of which also showed EGFr gene overex-pression, but not in well-differentiated gliomas or in nonneoplastic brain tis-sue specimens. No significant differ-ences in Ha-ras and Ki-ras expression were observed. Preliminary histochem-ical observations showed that intra-cellular levels of transforming growth factor α, a putative biochemical link between these two oncogenes, were significantly higher in glioblastoma cells than in controls.
AB - Five human glioblastoma cell lines were analyzed for oncogene activation with a panel of probes. Abnormal expression of the epidermal growth factor receptor (EGFr) gene was detected in four of five lines; N-ras oncogene overexpression was found in all five cell lines. These results were subsequently confirmed with fresh brain tumor and nonneoplastic brain tissue biopsy samples; increased expression of the N-ras proto-oncogene was observed in five of five glioblastomas, all of which also showed EGFr gene overex-pression, but not in well-differentiated gliomas or in nonneoplastic brain tis-sue specimens. No significant differ-ences in Ha-ras and Ki-ras expression were observed. Preliminary histochem-ical observations showed that intra-cellular levels of transforming growth factor α, a putative biochemical link between these two oncogenes, were significantly higher in glioblastoma cells than in controls.
UR - http://www.scopus.com/inward/record.url?scp=0024546277&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024546277&partnerID=8YFLogxK
U2 - 10.1093/jnci/81.1.63
DO - 10.1093/jnci/81.1.63
M3 - Article
VL - 81
SP - 63
EP - 67
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
SN - 0027-8874
IS - 1
ER -