Overexpression of n-ras oncogene and epidermal growth factor receptor gene in human glioblastomas

Massimo A. Gerosa, Marco Colombatti, Giuseppe Tridente, Daniela Talarico, Caterina Fognani, Elena Raimondi, Luigi De Carli, Giuliano Della Valle

Research output: Contribution to journalArticlepeer-review

Abstract

Five human glioblastoma cell lines were analyzed for oncogene activation with a panel of probes. Abnormal expression of the epidermal growth factor receptor (EGFr) gene was detected in four of five lines; N-ras oncogene overexpression was found in all five cell lines. These results were subsequently confirmed with fresh brain tumor and nonneoplastic brain tissue biopsy samples; increased expression of the N-ras proto-oncogene was observed in five of five glioblastomas, all of which also showed EGFr gene overex-pression, but not in well-differentiated gliomas or in nonneoplastic brain tis-sue specimens. No significant differ-ences in Ha-ras and Ki-ras expression were observed. Preliminary histochem-ical observations showed that intra-cellular levels of transforming growth factor α, a putative biochemical link between these two oncogenes, were significantly higher in glioblastoma cells than in controls.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalJournal of the National Cancer Institute
Volume81
Issue number1
DOIs
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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