Overexpression of p27BBP in head and neck carcinomas and their lymph node metastases

Paola Rosso, Giorgio Cortesina, Francesca Sanvito, Alessandra Donadini, Barbara Di Benedetto, Stefano Biffo, Pier Carlo Marchisio

Research output: Contribution to journalArticlepeer-review

Abstract

Background. p27BBP is a regulator of ribosome assembly and an essential nuclear and cytoplasmic component of eukaryotes. Methods. We investigated the immunochemical distribution of p27BBP in head and neck carcinomas, in the associated normal mucosa, and in regional lymph nodes. Results. p27BBP is detectable in mucosal cells but is overexpressed in carcinomas, highly concentrated in large polymorphous nucleoli, and even larger and more evident in lymph node metastatic foci. Western blotting confirms increased p27BBP in carcinomas versus normal mucosa and also in metastatic versus normal lymph nodes. The overexpression of p27 BBP corresponds to mRNA upregulation in carcinomas. Unexpectedly, a 52-kDa band specifically reacting with antibodies to p27BBP was observed in several carcinomas. Conclusions. p27BBP alterations are common events in the transition to malignancy and are probably involved in squamous carcinoma progression. Immune reagents raised to p27BBP may provide additional diagnostic tools for surgical pathology of tumor boundaries and lymph nodes. The 52-kDa band may represent an abnormal form of p27 BBP expressed by transformed airway epithelia.

Original languageEnglish
Pages (from-to)408-417
Number of pages10
JournalHead and Neck
Volume26
Issue number5
DOIs
Publication statusPublished - May 2004

Keywords

  • Head and neck carcinoma
  • Immunohistochemistry
  • Integrin β4
  • p27
  • Ribosome biogenesis

ASJC Scopus subject areas

  • Otorhinolaryngology

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