Overexpression of parkin rescues the defective mitochondrial phenotype and the increased apoptosis of Cockayne Syndrome A cells

Barbara Pascucci, Mariarosaria D'Errico, Alessandra Romagnoli, Chiara De Nuccio, Miriam Savino, Donatella Pietraforte, Manuela Lanzafame, Angelo Salvatore Calcagnile, Paola Fortini, Sara Baccarini, Donata Orioli, Paolo Degan, Sergio Visentin, Miria Stefanini, Ciro Isidoro, Gian Maria Fimia, Eugenia Dogliotti

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The ERCC8/CSA gene encodes a WD-40 repeat protein (CSA) that is part of a E3-ubiquitin ligase/COP9 signalosome complex. When mutated, CSA causes the Cockayne Syndrome group A (CS-A), a rare recessive progeroid disorder characterized by sun sensitivity and neurodevelopmental abnormalities. CS-A cells features include ROS hyperproduction, accumulation of oxidative genome damage, mitochondrial dysfunction and increased apoptosis that may contribute to the neurodegenerative process. In this study, we show that CSA localizes to mitochondria and specifically interacts with the mitochondrial fission protein dynamin-related protein (DRP1) that is hyperactivated when CSA is defective. Increased fission is not counterbalanced by increased mitophagy in CS-A cells thus leading to accumulation of fragmented mitochondria. However, when mitochondria are challenged with the mitochondrial toxin carbonyl cyanide m-chloro phenyl hydrazine, CS-A fibroblasts undergo mitophagy as efficiently as normal fibroblasts, suggesting that this process remains targetable to get rid of damaged mitochondria. Indeed, when basal mitophagy was potentiated by overexpressing Parkin in CSA deficient cells, a significant rescue of the dysfunctional mitochondrial phenotype was observed. Importantly, Parkin overexpression not only reactivates basal mitophagy, but plays also an anti-apoptotic role by significantly reducing the translocation of Bax at mitochondria in CS-A cells. These findings provide new mechanistic insights into the role of CSA in mitochondrial maintenance and might open new perspectives for therapeutic approaches.

Original languageEnglish
Pages (from-to)102852-102867
Number of pages16
JournalOncotarget
Volume8
Issue number61
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Cockayne Syndrome
Mitochondrial Degradation
Mitochondria
Apoptosis
Phenotype
hydrazine
Fibroblasts
Mitochondrial Dynamics
Dynamins
Ubiquitin-Protein Ligases
Mitochondrial Genome
Mitochondrial Proteins
Solar System
Proteins
Maintenance
Genes

Keywords

  • Cockayne syndrome
  • Mitochondrial dysfunction
  • Mitophagy
  • ROS

ASJC Scopus subject areas

  • Oncology

Cite this

Overexpression of parkin rescues the defective mitochondrial phenotype and the increased apoptosis of Cockayne Syndrome A cells. / Pascucci, Barbara; D'Errico, Mariarosaria; Romagnoli, Alessandra; De Nuccio, Chiara; Savino, Miriam; Pietraforte, Donatella; Lanzafame, Manuela; Calcagnile, Angelo Salvatore; Fortini, Paola; Baccarini, Sara; Orioli, Donata; Degan, Paolo; Visentin, Sergio; Stefanini, Miria; Isidoro, Ciro; Fimia, Gian Maria; Dogliotti, Eugenia.

In: Oncotarget, Vol. 8, No. 61, 01.01.2017, p. 102852-102867.

Research output: Contribution to journalArticle

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AU - D'Errico, Mariarosaria

AU - Romagnoli, Alessandra

AU - De Nuccio, Chiara

AU - Savino, Miriam

AU - Pietraforte, Donatella

AU - Lanzafame, Manuela

AU - Calcagnile, Angelo Salvatore

AU - Fortini, Paola

AU - Baccarini, Sara

AU - Orioli, Donata

AU - Degan, Paolo

AU - Visentin, Sergio

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AU - Isidoro, Ciro

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