Overexpression of the T-cell receptor β-chain variable region TCRBV14 in HLA-A2-matched primary human melanomas

S. Salvi, F. Segalla, S. Rao, F. Arienti, M. Sartori, G. Bratina, E. Caronni, A. Anichini, C. Clemente, G. Parmiani, M. Sensi

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Abstract

We have shown previously that peripheral blood lymphocytes (PBL) of patients with metastatic melanoma include cytotoxic T-cell closes that recognize Melan-A/MART-1 in a HIA-A2-restricted fashion. Such clones preferentially use the variable (V) regions TCRBV14 or TCRBV7 in the β- chain of their T-cell receptor (TCRB). It was not known, however, whether this finding is associated with the presence of the HLA-A2 allele in tumor tissue and whether evidence of the predominance of these TCRBV families can also be observed in primary tumor tissue. To address these issues, we have used a semiquantitative PCR to examine the TCRBV repertoire in six HLA-A2- matched primary melanomas in comparison with their autologous PBL. Although each patient had his or her own pattern of skewed TCRBV utilization, in all patients, T-cells that used TCRBV 14 were significantly overrepresented in the neoplastic site compared with PBL. All of the primary tumors studied had detectable expression of Melan-A/MART-1 and gp100, and immunohistochemical analysis confirmed the presence of the HLA-A2 allele. Additional samples of Melan-A/MART-1-positive, gp100-positive primary melanomas from six non-HLA- A2 patients and four autologous normal skin controls failed to reveal a TCRBV 14 predominance in such tissues. These results point tn a role of TCRBV14 T lymphocytes in the HLA-A2-restricted immune recognition of primary melanomas.

Original languageEnglish
Pages (from-to)3374-3379
Number of pages6
JournalCancer Research
Volume55
Issue number15
Publication statusPublished - 1995

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HLA-A2 Antigen
MART-1 Antigen
T-Cell Antigen Receptor
Melanoma
Lymphocytes
T-Lymphocytes
varespladib methyl
Alleles
Neoplasms
Clone Cells
Polymerase Chain Reaction
Skin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Overexpression of the T-cell receptor β-chain variable region TCRBV14 in HLA-A2-matched primary human melanomas. / Salvi, S.; Segalla, F.; Rao, S.; Arienti, F.; Sartori, M.; Bratina, G.; Caronni, E.; Anichini, A.; Clemente, C.; Parmiani, G.; Sensi, M.

In: Cancer Research, Vol. 55, No. 15, 1995, p. 3374-3379.

Research output: Contribution to journalArticle

Salvi, S, Segalla, F, Rao, S, Arienti, F, Sartori, M, Bratina, G, Caronni, E, Anichini, A, Clemente, C, Parmiani, G & Sensi, M 1995, 'Overexpression of the T-cell receptor β-chain variable region TCRBV14 in HLA-A2-matched primary human melanomas', Cancer Research, vol. 55, no. 15, pp. 3374-3379.
Salvi, S. ; Segalla, F. ; Rao, S. ; Arienti, F. ; Sartori, M. ; Bratina, G. ; Caronni, E. ; Anichini, A. ; Clemente, C. ; Parmiani, G. ; Sensi, M. / Overexpression of the T-cell receptor β-chain variable region TCRBV14 in HLA-A2-matched primary human melanomas. In: Cancer Research. 1995 ; Vol. 55, No. 15. pp. 3374-3379.
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T1 - Overexpression of the T-cell receptor β-chain variable region TCRBV14 in HLA-A2-matched primary human melanomas

AU - Salvi, S.

AU - Segalla, F.

AU - Rao, S.

AU - Arienti, F.

AU - Sartori, M.

AU - Bratina, G.

AU - Caronni, E.

AU - Anichini, A.

AU - Clemente, C.

AU - Parmiani, G.

AU - Sensi, M.

PY - 1995

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N2 - We have shown previously that peripheral blood lymphocytes (PBL) of patients with metastatic melanoma include cytotoxic T-cell closes that recognize Melan-A/MART-1 in a HIA-A2-restricted fashion. Such clones preferentially use the variable (V) regions TCRBV14 or TCRBV7 in the β- chain of their T-cell receptor (TCRB). It was not known, however, whether this finding is associated with the presence of the HLA-A2 allele in tumor tissue and whether evidence of the predominance of these TCRBV families can also be observed in primary tumor tissue. To address these issues, we have used a semiquantitative PCR to examine the TCRBV repertoire in six HLA-A2- matched primary melanomas in comparison with their autologous PBL. Although each patient had his or her own pattern of skewed TCRBV utilization, in all patients, T-cells that used TCRBV 14 were significantly overrepresented in the neoplastic site compared with PBL. All of the primary tumors studied had detectable expression of Melan-A/MART-1 and gp100, and immunohistochemical analysis confirmed the presence of the HLA-A2 allele. Additional samples of Melan-A/MART-1-positive, gp100-positive primary melanomas from six non-HLA- A2 patients and four autologous normal skin controls failed to reveal a TCRBV 14 predominance in such tissues. These results point tn a role of TCRBV14 T lymphocytes in the HLA-A2-restricted immune recognition of primary melanomas.

AB - We have shown previously that peripheral blood lymphocytes (PBL) of patients with metastatic melanoma include cytotoxic T-cell closes that recognize Melan-A/MART-1 in a HIA-A2-restricted fashion. Such clones preferentially use the variable (V) regions TCRBV14 or TCRBV7 in the β- chain of their T-cell receptor (TCRB). It was not known, however, whether this finding is associated with the presence of the HLA-A2 allele in tumor tissue and whether evidence of the predominance of these TCRBV families can also be observed in primary tumor tissue. To address these issues, we have used a semiquantitative PCR to examine the TCRBV repertoire in six HLA-A2- matched primary melanomas in comparison with their autologous PBL. Although each patient had his or her own pattern of skewed TCRBV utilization, in all patients, T-cells that used TCRBV 14 were significantly overrepresented in the neoplastic site compared with PBL. All of the primary tumors studied had detectable expression of Melan-A/MART-1 and gp100, and immunohistochemical analysis confirmed the presence of the HLA-A2 allele. Additional samples of Melan-A/MART-1-positive, gp100-positive primary melanomas from six non-HLA- A2 patients and four autologous normal skin controls failed to reveal a TCRBV 14 predominance in such tissues. These results point tn a role of TCRBV14 T lymphocytes in the HLA-A2-restricted immune recognition of primary melanomas.

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