TY - JOUR
T1 - Overexpression of tumor necrosis factor (TNF),α and TNFα receptor I in human viral myocariditis
T2 - Clinicopathologic correlations
AU - Calabrese, Fiorella
AU - Carturan, Elisa
AU - Chimenti, Cristina
AU - Pieroni, Maurizio
AU - Agostini, Carlo
AU - Angelini, Annalisa
AU - Crosato, Martino
AU - Valente, Marialuisa
AU - Boffa, Giovanni M.
AU - Frustaci, Andrea
AU - Thiene, Gaetano
PY - 2004/9
Y1 - 2004/9
N2 - Proinflammatory cytokines, including tumor necrosis factor (TNF)α, have been recognized as important physiopathogenetic factors in the inflammatory initiation and continuation of inflammatory cardiomyopathies. Experimental and preliminary human studies have demonstrated that TNFα plays a crucial role in enteroviral-induced myocarditis. In this study, we investigated the expression of TNFα and both its receptors (TNFRI and TNFRII) in both viral and nonviral myocarditis. Myocardial expression of TNFα was then correlated with different clinical and pathologic findings. TNFα expression was investigated in endomyocardial biopsies obtained from 38 patients with myocarditis and from eight control subjects by using reverse transcriptase-polymerase chain reaction (PCR) and immunohistochemistry. Viral etiology was diagnosed by PCR in 20 cases: enterovirus in seven, Epstein-Barr virus in four, hepatitis C virus in three, adenovirus in two, influenza virus in two, cytomegalovirus in one, and double infection adenovirus and enterovirus in one. Immunohistochemistry was also used to analyze both TNFα receptors (RI and RII). A semiquantitative analysis was employed (score 0-3) for necrosis, inflammation, fibrosis and immunohistochemical findings. TNFα mRNA and TNFα protein were significantly more present in viral myocarditis than in nonviral myocarditis (16/20 vs 3/18, P = 0.001). Remarkable immunostaining was observed for both receptors, particularly TNFRI. Histological analysis revealed that myocardial necrosis (mean score 1.89 vs 1.15, P = 0.01) and cellular infiltration (mean score 2.26 vs 1.78, P = 0.05) were more prominent in TNFα-positive cases. Among TNFα-positive cases, the greater TNFα mRNAs, the more impaired was cardiac function. Our findings suggest that the expression of TNFα may play an important role in the pathogenesis of viral myocarditis of any etiology and may influence the severity of cardiac dysfunction. Cytokine effects are more strictly linked to overexpression of TNFRI.
AB - Proinflammatory cytokines, including tumor necrosis factor (TNF)α, have been recognized as important physiopathogenetic factors in the inflammatory initiation and continuation of inflammatory cardiomyopathies. Experimental and preliminary human studies have demonstrated that TNFα plays a crucial role in enteroviral-induced myocarditis. In this study, we investigated the expression of TNFα and both its receptors (TNFRI and TNFRII) in both viral and nonviral myocarditis. Myocardial expression of TNFα was then correlated with different clinical and pathologic findings. TNFα expression was investigated in endomyocardial biopsies obtained from 38 patients with myocarditis and from eight control subjects by using reverse transcriptase-polymerase chain reaction (PCR) and immunohistochemistry. Viral etiology was diagnosed by PCR in 20 cases: enterovirus in seven, Epstein-Barr virus in four, hepatitis C virus in three, adenovirus in two, influenza virus in two, cytomegalovirus in one, and double infection adenovirus and enterovirus in one. Immunohistochemistry was also used to analyze both TNFα receptors (RI and RII). A semiquantitative analysis was employed (score 0-3) for necrosis, inflammation, fibrosis and immunohistochemical findings. TNFα mRNA and TNFα protein were significantly more present in viral myocarditis than in nonviral myocarditis (16/20 vs 3/18, P = 0.001). Remarkable immunostaining was observed for both receptors, particularly TNFRI. Histological analysis revealed that myocardial necrosis (mean score 1.89 vs 1.15, P = 0.01) and cellular infiltration (mean score 2.26 vs 1.78, P = 0.05) were more prominent in TNFα-positive cases. Among TNFα-positive cases, the greater TNFα mRNAs, the more impaired was cardiac function. Our findings suggest that the expression of TNFα may play an important role in the pathogenesis of viral myocarditis of any etiology and may influence the severity of cardiac dysfunction. Cytokine effects are more strictly linked to overexpression of TNFRI.
KW - TNFα
KW - TNFα receptors
KW - Viral myocarditis
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U2 - 10.1038/modpathol.3800158
DO - 10.1038/modpathol.3800158
M3 - Article
C2 - 15218506
AN - SCOPUS:4344627129
VL - 17
SP - 1108
EP - 1118
JO - Modern Pathology
JF - Modern Pathology
SN - 0893-3952
IS - 9
ER -