Overexpression of TWIST2 correlates with poor prognosis in head and neck squamous cell carcinomas

Daniela Gasparotto, Jerry Polesel, Alessandra Marzotto, Roberta Colladel, Sara Piccinin, Piergiorgio Modena, Alessandra Grizzo, Sandro Sulfaro, Diego Serraino, Luigi Barzan, Claudio Doglioni, Roberta Maestro

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Head and neck squamous cell carcinomas (HNSCC) are a heterogeneous group of tumors with variable presentation and clinical behavior. Despite improvements in surgical and radiation therapy techniques, the 5-year survival rate has not improved significantly over the past decades. Thus, there is an urgent need to identify novel markers that may allow for the development of personalized therapeutic approaches. In the present study we evaluated the prognostic role of the expression of genes related to the induction of epithelial mesenchymal transition (EMT). To this aim, a consecutive series of 69 HNSCC were analyzed for the expression of TWIST1, TWIST2, SNAI1, SNAI2, E-Cadherin, N-Cadherin and Vimentin. TWIST1, TWIST2, SNAI1 and SNAI2 were significantly overexpressed in HNSCC, with TWIST2, SNAI1 and SNAI2 being more markedly increased in tumors compared to normal mucosae. The expression of TWIST1 and SNAI2 was associated with upregulation of mesenchymal markers, but failed to correlate with pathological parameters or clinical behaviour. In contrast, we found that upregulation of TWIST2, which was independent of the activation of a mesenchymal differentiation program, correlated with poor differentiation grade (p=0.016) and shorter survival (p=0.025), and identifies a subset of nodepositive oral cavity/pharynx cancer patients with very poor prognosis (p

Original languageEnglish
Pages (from-to)1165-1175
Number of pages11
JournalOncotarget
Volume2
Issue number12
Publication statusPublished - Dec 2011

Fingerprint

Cadherins
Up-Regulation
Pharyngeal Neoplasms
Epithelial-Mesenchymal Transition
Vimentin
Mouth
Neoplasms
Mucous Membrane
Radiotherapy
Survival Rate
Gene Expression
Survival
Carcinoma, squamous cell of head and neck
Therapeutics

Keywords

  • EMT
  • HNSCC
  • SNAI1
  • SNAI2
  • TWIST1
  • TWIST2

ASJC Scopus subject areas

  • Oncology

Cite this

Gasparotto, D., Polesel, J., Marzotto, A., Colladel, R., Piccinin, S., Modena, P., ... Maestro, R. (2011). Overexpression of TWIST2 correlates with poor prognosis in head and neck squamous cell carcinomas. Oncotarget, 2(12), 1165-1175.

Overexpression of TWIST2 correlates with poor prognosis in head and neck squamous cell carcinomas. / Gasparotto, Daniela; Polesel, Jerry; Marzotto, Alessandra; Colladel, Roberta; Piccinin, Sara; Modena, Piergiorgio; Grizzo, Alessandra; Sulfaro, Sandro; Serraino, Diego; Barzan, Luigi; Doglioni, Claudio; Maestro, Roberta.

In: Oncotarget, Vol. 2, No. 12, 12.2011, p. 1165-1175.

Research output: Contribution to journalArticle

Gasparotto, D, Polesel, J, Marzotto, A, Colladel, R, Piccinin, S, Modena, P, Grizzo, A, Sulfaro, S, Serraino, D, Barzan, L, Doglioni, C & Maestro, R 2011, 'Overexpression of TWIST2 correlates with poor prognosis in head and neck squamous cell carcinomas', Oncotarget, vol. 2, no. 12, pp. 1165-1175.
Gasparotto D, Polesel J, Marzotto A, Colladel R, Piccinin S, Modena P et al. Overexpression of TWIST2 correlates with poor prognosis in head and neck squamous cell carcinomas. Oncotarget. 2011 Dec;2(12):1165-1175.
Gasparotto, Daniela ; Polesel, Jerry ; Marzotto, Alessandra ; Colladel, Roberta ; Piccinin, Sara ; Modena, Piergiorgio ; Grizzo, Alessandra ; Sulfaro, Sandro ; Serraino, Diego ; Barzan, Luigi ; Doglioni, Claudio ; Maestro, Roberta. / Overexpression of TWIST2 correlates with poor prognosis in head and neck squamous cell carcinomas. In: Oncotarget. 2011 ; Vol. 2, No. 12. pp. 1165-1175.
@article{3a48ab945fd14bbbba1b0fbbefc6041e,
title = "Overexpression of TWIST2 correlates with poor prognosis in head and neck squamous cell carcinomas",
abstract = "Head and neck squamous cell carcinomas (HNSCC) are a heterogeneous group of tumors with variable presentation and clinical behavior. Despite improvements in surgical and radiation therapy techniques, the 5-year survival rate has not improved significantly over the past decades. Thus, there is an urgent need to identify novel markers that may allow for the development of personalized therapeutic approaches. In the present study we evaluated the prognostic role of the expression of genes related to the induction of epithelial mesenchymal transition (EMT). To this aim, a consecutive series of 69 HNSCC were analyzed for the expression of TWIST1, TWIST2, SNAI1, SNAI2, E-Cadherin, N-Cadherin and Vimentin. TWIST1, TWIST2, SNAI1 and SNAI2 were significantly overexpressed in HNSCC, with TWIST2, SNAI1 and SNAI2 being more markedly increased in tumors compared to normal mucosae. The expression of TWIST1 and SNAI2 was associated with upregulation of mesenchymal markers, but failed to correlate with pathological parameters or clinical behaviour. In contrast, we found that upregulation of TWIST2, which was independent of the activation of a mesenchymal differentiation program, correlated with poor differentiation grade (p=0.016) and shorter survival (p=0.025), and identifies a subset of nodepositive oral cavity/pharynx cancer patients with very poor prognosis (p",
keywords = "EMT, HNSCC, SNAI1, SNAI2, TWIST1, TWIST2",
author = "Daniela Gasparotto and Jerry Polesel and Alessandra Marzotto and Roberta Colladel and Sara Piccinin and Piergiorgio Modena and Alessandra Grizzo and Sandro Sulfaro and Diego Serraino and Luigi Barzan and Claudio Doglioni and Roberta Maestro",
year = "2011",
month = "12",
language = "English",
volume = "2",
pages = "1165--1175",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals LLC",
number = "12",

}

TY - JOUR

T1 - Overexpression of TWIST2 correlates with poor prognosis in head and neck squamous cell carcinomas

AU - Gasparotto, Daniela

AU - Polesel, Jerry

AU - Marzotto, Alessandra

AU - Colladel, Roberta

AU - Piccinin, Sara

AU - Modena, Piergiorgio

AU - Grizzo, Alessandra

AU - Sulfaro, Sandro

AU - Serraino, Diego

AU - Barzan, Luigi

AU - Doglioni, Claudio

AU - Maestro, Roberta

PY - 2011/12

Y1 - 2011/12

N2 - Head and neck squamous cell carcinomas (HNSCC) are a heterogeneous group of tumors with variable presentation and clinical behavior. Despite improvements in surgical and radiation therapy techniques, the 5-year survival rate has not improved significantly over the past decades. Thus, there is an urgent need to identify novel markers that may allow for the development of personalized therapeutic approaches. In the present study we evaluated the prognostic role of the expression of genes related to the induction of epithelial mesenchymal transition (EMT). To this aim, a consecutive series of 69 HNSCC were analyzed for the expression of TWIST1, TWIST2, SNAI1, SNAI2, E-Cadherin, N-Cadherin and Vimentin. TWIST1, TWIST2, SNAI1 and SNAI2 were significantly overexpressed in HNSCC, with TWIST2, SNAI1 and SNAI2 being more markedly increased in tumors compared to normal mucosae. The expression of TWIST1 and SNAI2 was associated with upregulation of mesenchymal markers, but failed to correlate with pathological parameters or clinical behaviour. In contrast, we found that upregulation of TWIST2, which was independent of the activation of a mesenchymal differentiation program, correlated with poor differentiation grade (p=0.016) and shorter survival (p=0.025), and identifies a subset of nodepositive oral cavity/pharynx cancer patients with very poor prognosis (p

AB - Head and neck squamous cell carcinomas (HNSCC) are a heterogeneous group of tumors with variable presentation and clinical behavior. Despite improvements in surgical and radiation therapy techniques, the 5-year survival rate has not improved significantly over the past decades. Thus, there is an urgent need to identify novel markers that may allow for the development of personalized therapeutic approaches. In the present study we evaluated the prognostic role of the expression of genes related to the induction of epithelial mesenchymal transition (EMT). To this aim, a consecutive series of 69 HNSCC were analyzed for the expression of TWIST1, TWIST2, SNAI1, SNAI2, E-Cadherin, N-Cadherin and Vimentin. TWIST1, TWIST2, SNAI1 and SNAI2 were significantly overexpressed in HNSCC, with TWIST2, SNAI1 and SNAI2 being more markedly increased in tumors compared to normal mucosae. The expression of TWIST1 and SNAI2 was associated with upregulation of mesenchymal markers, but failed to correlate with pathological parameters or clinical behaviour. In contrast, we found that upregulation of TWIST2, which was independent of the activation of a mesenchymal differentiation program, correlated with poor differentiation grade (p=0.016) and shorter survival (p=0.025), and identifies a subset of nodepositive oral cavity/pharynx cancer patients with very poor prognosis (p

KW - EMT

KW - HNSCC

KW - SNAI1

KW - SNAI2

KW - TWIST1

KW - TWIST2

UR - http://www.scopus.com/inward/record.url?scp=84863516199&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863516199&partnerID=8YFLogxK

M3 - Article

C2 - 22201613

AN - SCOPUS:84863516199

VL - 2

SP - 1165

EP - 1175

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 12

ER -