The Aeromonas hydrophila CphA metallo-β-lactamase was overexpressed in a soluble secreted form in Escherichia coli using a T7 RNA polymerase-based expression system, and a simple protocol based on a single cation-exchange chromatographic step was developed, which is suitable for rapid purification of the overexpressed enzyme from E. coli lysates. A yield of up to 30 μg of purified enzyme per milliliter of culture was obtained. The purified enzyme preparation showed properties identical to those previously reported in the literature.
|Number of pages||4|
|Journal||Microbial Drug Resistance|
|Publication status||Published - 1996|
ASJC Scopus subject areas
- Microbiology (medical)