Oxidation of ferulic acid by laccase: Identification of the products and inhibitory effects of some dipeptides

Federica Carunchio, Carlo Crescenzi, Anna Maria Girelli, Antonella Messina, Anna Maria Tarola

Research output: Contribution to journalArticle

Abstract

The laccase activity, in the presence of ferulic acid as substrate, was tested with high performance liquid chromatography (HPLC). The two products initially formed were identified by HPLC-mass spectrometry and HPLC-diode array as trans-5-[(E)-2-carboxyvinyl]-2-(4-hydroxy-3-methoxy-phenyl) -7-methoxy-2,3-dihydrobenzofuran-3-carboxylic acid and (Z)-β-{4-[(E)-2-carboxyvinyl]-2-methoxyphenoxy}-4-hydroxy-3-methoxy cinnamic acid. The inhibitory effects of some dipeptides due to the formation of copper complexes have also been reported. The results obtained confirm that the inhibitory effects on laccase activity are strongly related to the structure of the dipeptides and confirm the important role of copper(II) in the reaction mechanism.

Original languageEnglish
Pages (from-to)189-200
Number of pages12
JournalTalanta
Volume55
Issue number1
DOIs
Publication statusPublished - Aug 3 2001

Keywords

  • Dipeptides
  • High performance liquid chromatography
  • Inhibitors
  • Laccase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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