TY - JOUR
T1 - Oxidation of ferulic acid by laccase
T2 - Identification of the products and inhibitory effects of some dipeptides
AU - Carunchio, Federica
AU - Crescenzi, Carlo
AU - Girelli, Anna Maria
AU - Messina, Antonella
AU - Tarola, Anna Maria
PY - 2001/8/3
Y1 - 2001/8/3
N2 - The laccase activity, in the presence of ferulic acid as substrate, was tested with high performance liquid chromatography (HPLC). The two products initially formed were identified by HPLC-mass spectrometry and HPLC-diode array as trans-5-[(E)-2-carboxyvinyl]-2-(4-hydroxy-3-methoxy-phenyl) -7-methoxy-2,3-dihydrobenzofuran-3-carboxylic acid and (Z)-β-{4-[(E)-2-carboxyvinyl]-2-methoxyphenoxy}-4-hydroxy-3-methoxy cinnamic acid. The inhibitory effects of some dipeptides due to the formation of copper complexes have also been reported. The results obtained confirm that the inhibitory effects on laccase activity are strongly related to the structure of the dipeptides and confirm the important role of copper(II) in the reaction mechanism.
AB - The laccase activity, in the presence of ferulic acid as substrate, was tested with high performance liquid chromatography (HPLC). The two products initially formed were identified by HPLC-mass spectrometry and HPLC-diode array as trans-5-[(E)-2-carboxyvinyl]-2-(4-hydroxy-3-methoxy-phenyl) -7-methoxy-2,3-dihydrobenzofuran-3-carboxylic acid and (Z)-β-{4-[(E)-2-carboxyvinyl]-2-methoxyphenoxy}-4-hydroxy-3-methoxy cinnamic acid. The inhibitory effects of some dipeptides due to the formation of copper complexes have also been reported. The results obtained confirm that the inhibitory effects on laccase activity are strongly related to the structure of the dipeptides and confirm the important role of copper(II) in the reaction mechanism.
KW - Dipeptides
KW - High performance liquid chromatography
KW - Inhibitors
KW - Laccase
UR - http://www.scopus.com/inward/record.url?scp=0035800642&partnerID=8YFLogxK
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U2 - 10.1016/S0039-9140(01)00417-9
DO - 10.1016/S0039-9140(01)00417-9
M3 - Article
AN - SCOPUS:0035800642
VL - 55
SP - 189
EP - 200
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 1
ER -