Oxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet

Gaetano Serviddio, Anna M. Giudetti, Francesco Bellanti, Paola Priore, Tiziana Rollo, Rosanna Tamborra, Luisa Siculella, Gianluigi Vendemiale, Emanuele Altomare, Gabriele V. Gnoni

Research output: Contribution to journalArticle

Abstract

There is growing evidence that mitochondrial dysfunction, and more specifically fatty acid β-oxidationimpairment, is involved in the pathophysiology of non-alcoholic steatohepatitis (NASH). The goal of the present study was to achieve more understanding on the modification/s of carnitinepalmitoyltransferase-I (CPT-I), the rate-limiting enzyme of the mitochondrial fatty acid β-oxidation, duringsteatohepatitis. A high fat/methionine-choline deficient (MCD) diet, administered for 4 weeks, was used to induce NASH in rats.We demonstrated that CPT-Iactivitydecreased, to the same extent, both in isolated liver mitochondria and in digitonin-permeabilized hepatocytes from MCD-diet fed rats.At the same time, the rate of total fatty acid oxidation to CO 2 and ketone bodies, measured in isolated hepatocytes, was significantly lowered in treated animalswhen compared to controls. Finally, an increase in CPT-I mRNA abundance and protein content, together with a high level of CPT-I protein oxidation was observed in treated rats. A posttranslational modification of rat CPT-I during steatohepatitishas been here discussed.

Original languageEnglish
Article numbere24084
JournalPLoS One
Volume6
Issue number9
DOIs
Publication statusPublished - Sep 1 2011

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Fingerprint Dive into the research topics of 'Oxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet'. Together they form a unique fingerprint.

  • Cite this

    Serviddio, G., Giudetti, A. M., Bellanti, F., Priore, P., Rollo, T., Tamborra, R., Siculella, L., Vendemiale, G., Altomare, E., & Gnoni, G. V. (2011). Oxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet. PLoS One, 6(9), [e24084]. https://doi.org/10.1371/journal.pone.0024084