Oxidative damage in DNA bases revealed by UV resonant Raman spectroscopy

Francesco D'Amico, Francesca Cammisuli, Riccardo Addobbati, Clara Rizzardi, Alessandro Gessini, Claudio Masciovecchio, Barbara Rossi, Lorella Pascolo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We report on the use of the UV Raman technique to monitor the oxidative damage of deoxynucleotide triphosphates (dATP, dGTP, dCTP and dTTP) and DNA (plasmid vector) solutions. Nucleotide and DNA aqueous solutions were exposed to hydrogen peroxide (H2O2) and iron containing carbon nanotubes (CNTs) to produce Fenton's reaction and induce oxidative damage. UV Raman spectroscopy is shown to be maximally efficient to reveal changes in the nitrogenous bases during the oxidative mechanisms occurring on these molecules. The analysis of Raman spectra, supported by numerical computations, revealed that the Fenton's reaction causes an oxidation of the nitrogenous bases in dATP, dGTP and dCTP solutions leading to the production of 2-hydroxyadenine, 8-hydroxyguanine and 5-hydroxycytosine. No thymine change was revealed in the dTTP solution under the same conditions. Compared to single nucleotide solutions, plasmid DNA oxidation has resulted in more radical damage that causes the breaking of the adenine and guanine aromatic rings. Our study demonstrates the advantage of using UV Raman spectroscopy for rapidly monitoring the oxidation changes in DNA aqueous solutions that can be assigned to specific nitrogenous bases. This journal is

Original languageEnglish
Pages (from-to)1477-1485
Number of pages9
JournalAnalyst
Volume140
Issue number5
DOIs
Publication statusPublished - Mar 7 2015

Fingerprint

Raman Spectrum Analysis
Raman spectroscopy
DNA Damage
DNA
damage
plasmid
oxidation
aqueous solution
Nucleotides
Ultraviolet spectroscopy
Oxidation
Plasmids
hydrogen peroxide
Carbon Nanotubes
Thymine
Guanine
Adenine
Hydrogen peroxide
iron
Hydrogen Peroxide

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry
  • Medicine(all)

Cite this

D'Amico, F., Cammisuli, F., Addobbati, R., Rizzardi, C., Gessini, A., Masciovecchio, C., ... Pascolo, L. (2015). Oxidative damage in DNA bases revealed by UV resonant Raman spectroscopy. Analyst, 140(5), 1477-1485. https://doi.org/10.1039/c4an02364a

Oxidative damage in DNA bases revealed by UV resonant Raman spectroscopy. / D'Amico, Francesco; Cammisuli, Francesca; Addobbati, Riccardo; Rizzardi, Clara; Gessini, Alessandro; Masciovecchio, Claudio; Rossi, Barbara; Pascolo, Lorella.

In: Analyst, Vol. 140, No. 5, 07.03.2015, p. 1477-1485.

Research output: Contribution to journalArticle

D'Amico, F, Cammisuli, F, Addobbati, R, Rizzardi, C, Gessini, A, Masciovecchio, C, Rossi, B & Pascolo, L 2015, 'Oxidative damage in DNA bases revealed by UV resonant Raman spectroscopy', Analyst, vol. 140, no. 5, pp. 1477-1485. https://doi.org/10.1039/c4an02364a
D'Amico F, Cammisuli F, Addobbati R, Rizzardi C, Gessini A, Masciovecchio C et al. Oxidative damage in DNA bases revealed by UV resonant Raman spectroscopy. Analyst. 2015 Mar 7;140(5):1477-1485. https://doi.org/10.1039/c4an02364a
D'Amico, Francesco ; Cammisuli, Francesca ; Addobbati, Riccardo ; Rizzardi, Clara ; Gessini, Alessandro ; Masciovecchio, Claudio ; Rossi, Barbara ; Pascolo, Lorella. / Oxidative damage in DNA bases revealed by UV resonant Raman spectroscopy. In: Analyst. 2015 ; Vol. 140, No. 5. pp. 1477-1485.
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