Oxidative Damage of the Gastric Mucosa in Helicobacter pylori Positive Chronic Atrophic and Nonatrophic Gastritis, Before and After Eradication

Federico Iacopini, Adriana Consolazio, Daniela Bosco, Adriana Marcheggiano, Antonino Bella, Roberta Pica, Omero A. Paoluzi, Pietro Crispino, Margherita Rivera, Marcella Mottolese, Francesco Nardi, Paolo Paoluzi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background. Helicobacter pylori is the main cause of gastritis and a primary carcinogen. The aim of this study was to assess oxidative damage in mucosal compartments of gastric mucosa in H. pylori positive and negative atrophic and nonatrophic gastritis. Materials and methods. Five groups of 10 patients each were identified according to H. pylori positive or negative chronic atrophic (Hp-CAG and CAG, respectively) and nonatrophic gastritis (Hp-CG and CG, respectively), and H. pylori negative normal mucosa (controls). Oxidative damage was evaluated by nitrotyrosine immunohistochemistry in the whole mucosa and in each compartment at baseline and at 2 and 12 months after eradication. Types of intestinal metaplasia were classified by histochemistry. Results. Total nitrotyrosine levels appeared significantly higher in H. pylori positive than in negative patients, and in Hp-CAG than in Hp-CG (p <.001); no differences were found between H. pylori negative gastritis and normal mucosa. Nitrotyrosine were found in foveolae and intestinal metaplasia only in Hp-CAG. At 12 months after H. pylori eradication, total nitrotyrosine levels showed a trend toward a decrease in Hp-CG and decreased significantly in Hp-CAG (p = .002), disappearing from the foveolae (p = .002), but remaining unchanged in intestinal metaplasia. Type I and II of intestinal metaplasia were present with the same prevalence in Hp-CAG and CAG, and did not change after H. pylori eradication. Conclusions. Oxidative damage of the gastric mucosa increases from Hp-CG to Hp-CAG, involving the foveolae and intestinal metaplasia. H. pylori eradication induces a complete healing of foveolae but not of intestinal metaplasia, reducing the overall oxidative damage in the mucosa.

Original languageEnglish
Pages (from-to)503-512
Number of pages10
JournalHelicobacter
Volume8
Issue number5
DOIs
Publication statusPublished - 2003

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Atrophic Gastritis
Gastric Mucosa
Helicobacter pylori
Metaplasia
Mucous Membrane
Gastritis
Carcinogens
Immunohistochemistry
3-nitrotyrosine

Keywords

  • Carcinogenesis
  • Chronic gastritis
  • Helicobacter pylori
  • Nitrotyrosine
  • Oxidative damage

ASJC Scopus subject areas

  • Gastroenterology
  • Microbiology

Cite this

Oxidative Damage of the Gastric Mucosa in Helicobacter pylori Positive Chronic Atrophic and Nonatrophic Gastritis, Before and After Eradication. / Iacopini, Federico; Consolazio, Adriana; Bosco, Daniela; Marcheggiano, Adriana; Bella, Antonino; Pica, Roberta; Paoluzi, Omero A.; Crispino, Pietro; Rivera, Margherita; Mottolese, Marcella; Nardi, Francesco; Paoluzi, Paolo.

In: Helicobacter, Vol. 8, No. 5, 2003, p. 503-512.

Research output: Contribution to journalArticle

Iacopini, F, Consolazio, A, Bosco, D, Marcheggiano, A, Bella, A, Pica, R, Paoluzi, OA, Crispino, P, Rivera, M, Mottolese, M, Nardi, F & Paoluzi, P 2003, 'Oxidative Damage of the Gastric Mucosa in Helicobacter pylori Positive Chronic Atrophic and Nonatrophic Gastritis, Before and After Eradication', Helicobacter, vol. 8, no. 5, pp. 503-512. https://doi.org/10.1046/j.1523-5378.2003.00172.x
Iacopini, Federico ; Consolazio, Adriana ; Bosco, Daniela ; Marcheggiano, Adriana ; Bella, Antonino ; Pica, Roberta ; Paoluzi, Omero A. ; Crispino, Pietro ; Rivera, Margherita ; Mottolese, Marcella ; Nardi, Francesco ; Paoluzi, Paolo. / Oxidative Damage of the Gastric Mucosa in Helicobacter pylori Positive Chronic Atrophic and Nonatrophic Gastritis, Before and After Eradication. In: Helicobacter. 2003 ; Vol. 8, No. 5. pp. 503-512.
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T1 - Oxidative Damage of the Gastric Mucosa in Helicobacter pylori Positive Chronic Atrophic and Nonatrophic Gastritis, Before and After Eradication

AU - Iacopini, Federico

AU - Consolazio, Adriana

AU - Bosco, Daniela

AU - Marcheggiano, Adriana

AU - Bella, Antonino

AU - Pica, Roberta

AU - Paoluzi, Omero A.

AU - Crispino, Pietro

AU - Rivera, Margherita

AU - Mottolese, Marcella

AU - Nardi, Francesco

AU - Paoluzi, Paolo

PY - 2003

Y1 - 2003

N2 - Background. Helicobacter pylori is the main cause of gastritis and a primary carcinogen. The aim of this study was to assess oxidative damage in mucosal compartments of gastric mucosa in H. pylori positive and negative atrophic and nonatrophic gastritis. Materials and methods. Five groups of 10 patients each were identified according to H. pylori positive or negative chronic atrophic (Hp-CAG and CAG, respectively) and nonatrophic gastritis (Hp-CG and CG, respectively), and H. pylori negative normal mucosa (controls). Oxidative damage was evaluated by nitrotyrosine immunohistochemistry in the whole mucosa and in each compartment at baseline and at 2 and 12 months after eradication. Types of intestinal metaplasia were classified by histochemistry. Results. Total nitrotyrosine levels appeared significantly higher in H. pylori positive than in negative patients, and in Hp-CAG than in Hp-CG (p <.001); no differences were found between H. pylori negative gastritis and normal mucosa. Nitrotyrosine were found in foveolae and intestinal metaplasia only in Hp-CAG. At 12 months after H. pylori eradication, total nitrotyrosine levels showed a trend toward a decrease in Hp-CG and decreased significantly in Hp-CAG (p = .002), disappearing from the foveolae (p = .002), but remaining unchanged in intestinal metaplasia. Type I and II of intestinal metaplasia were present with the same prevalence in Hp-CAG and CAG, and did not change after H. pylori eradication. Conclusions. Oxidative damage of the gastric mucosa increases from Hp-CG to Hp-CAG, involving the foveolae and intestinal metaplasia. H. pylori eradication induces a complete healing of foveolae but not of intestinal metaplasia, reducing the overall oxidative damage in the mucosa.

AB - Background. Helicobacter pylori is the main cause of gastritis and a primary carcinogen. The aim of this study was to assess oxidative damage in mucosal compartments of gastric mucosa in H. pylori positive and negative atrophic and nonatrophic gastritis. Materials and methods. Five groups of 10 patients each were identified according to H. pylori positive or negative chronic atrophic (Hp-CAG and CAG, respectively) and nonatrophic gastritis (Hp-CG and CG, respectively), and H. pylori negative normal mucosa (controls). Oxidative damage was evaluated by nitrotyrosine immunohistochemistry in the whole mucosa and in each compartment at baseline and at 2 and 12 months after eradication. Types of intestinal metaplasia were classified by histochemistry. Results. Total nitrotyrosine levels appeared significantly higher in H. pylori positive than in negative patients, and in Hp-CAG than in Hp-CG (p <.001); no differences were found between H. pylori negative gastritis and normal mucosa. Nitrotyrosine were found in foveolae and intestinal metaplasia only in Hp-CAG. At 12 months after H. pylori eradication, total nitrotyrosine levels showed a trend toward a decrease in Hp-CG and decreased significantly in Hp-CAG (p = .002), disappearing from the foveolae (p = .002), but remaining unchanged in intestinal metaplasia. Type I and II of intestinal metaplasia were present with the same prevalence in Hp-CAG and CAG, and did not change after H. pylori eradication. Conclusions. Oxidative damage of the gastric mucosa increases from Hp-CG to Hp-CAG, involving the foveolae and intestinal metaplasia. H. pylori eradication induces a complete healing of foveolae but not of intestinal metaplasia, reducing the overall oxidative damage in the mucosa.

KW - Carcinogenesis

KW - Chronic gastritis

KW - Helicobacter pylori

KW - Nitrotyrosine

KW - Oxidative damage

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