Oxidative DNA damage in the human trabecular meshwork: Clinical correlation in patients with primary open-angle glaucoma

Sergio Claudio Saccà, Antonio Pascotto, Paolo Camicione, Paolo Capris, Alberto Izzotti

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To evaluate the intensity of oxidative molecular damage and its clinical correlations: visual field damage, intraocular pressure, age, and disease duration. Methods: DNA was extracted from human trabecular meshwork specimens collected from 17 glaucoma-affected patients using standard filtration surgery. Twenty-one specimens from healthy eyes collected for cornea transplants serve as controls. Oxidative DNA damage was evaluated by determining 8-hydroxy-2′-deoxyguanosine levels. All patients underwent a Humphrey 30-2 visual field examination and diurnal tonometry before surgery. Results: The mean ± SD DNA oxidative damage was 8.51 ± 5.44 and 1.75 ± 1.80 8-hydroxy-2′-deoxyguanosine molecules/105 normal nucleotides in patients with glaucoma and controls, respectively. A statistically significant correlation was found among human trabecular meshwork DNA oxidative damage, visual field damage, and intraocular pressure. No other statistically significant correlations were found. Conclusions: Oxidative stress may represent an important pathogenetic step in primary open-angle glaucoma because it could induce human trabecular meshwork degeneration, favoring an intraocular pressure increase, thus priming the glaucoma pathogenetic cascade.

Original languageEnglish
Pages (from-to)458-463
Number of pages6
JournalArchives of Ophthalmology
Volume123
Issue number4
DOIs
Publication statusPublished - Apr 2005

ASJC Scopus subject areas

  • Ophthalmology

Fingerprint Dive into the research topics of 'Oxidative DNA damage in the human trabecular meshwork: Clinical correlation in patients with primary open-angle glaucoma'. Together they form a unique fingerprint.

Cite this