TY - JOUR
T1 - Oxidative DNA damage in the human trabecular meshwork
T2 - Clinical correlation in patients with primary open-angle glaucoma
AU - Saccà, Sergio Claudio
AU - Pascotto, Antonio
AU - Camicione, Paolo
AU - Capris, Paolo
AU - Izzotti, Alberto
PY - 2005/4
Y1 - 2005/4
N2 - Objective: To evaluate the intensity of oxidative molecular damage and its clinical correlations: visual field damage, intraocular pressure, age, and disease duration. Methods: DNA was extracted from human trabecular meshwork specimens collected from 17 glaucoma-affected patients using standard filtration surgery. Twenty-one specimens from healthy eyes collected for cornea transplants serve as controls. Oxidative DNA damage was evaluated by determining 8-hydroxy-2′-deoxyguanosine levels. All patients underwent a Humphrey 30-2 visual field examination and diurnal tonometry before surgery. Results: The mean ± SD DNA oxidative damage was 8.51 ± 5.44 and 1.75 ± 1.80 8-hydroxy-2′-deoxyguanosine molecules/105 normal nucleotides in patients with glaucoma and controls, respectively. A statistically significant correlation was found among human trabecular meshwork DNA oxidative damage, visual field damage, and intraocular pressure. No other statistically significant correlations were found. Conclusions: Oxidative stress may represent an important pathogenetic step in primary open-angle glaucoma because it could induce human trabecular meshwork degeneration, favoring an intraocular pressure increase, thus priming the glaucoma pathogenetic cascade.
AB - Objective: To evaluate the intensity of oxidative molecular damage and its clinical correlations: visual field damage, intraocular pressure, age, and disease duration. Methods: DNA was extracted from human trabecular meshwork specimens collected from 17 glaucoma-affected patients using standard filtration surgery. Twenty-one specimens from healthy eyes collected for cornea transplants serve as controls. Oxidative DNA damage was evaluated by determining 8-hydroxy-2′-deoxyguanosine levels. All patients underwent a Humphrey 30-2 visual field examination and diurnal tonometry before surgery. Results: The mean ± SD DNA oxidative damage was 8.51 ± 5.44 and 1.75 ± 1.80 8-hydroxy-2′-deoxyguanosine molecules/105 normal nucleotides in patients with glaucoma and controls, respectively. A statistically significant correlation was found among human trabecular meshwork DNA oxidative damage, visual field damage, and intraocular pressure. No other statistically significant correlations were found. Conclusions: Oxidative stress may represent an important pathogenetic step in primary open-angle glaucoma because it could induce human trabecular meshwork degeneration, favoring an intraocular pressure increase, thus priming the glaucoma pathogenetic cascade.
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U2 - 10.1001/archopht.123.4.458
DO - 10.1001/archopht.123.4.458
M3 - Article
C2 - 15824217
AN - SCOPUS:16844379509
VL - 123
SP - 458
EP - 463
JO - Archives of Ophthalmology
JF - Archives of Ophthalmology
SN - 0003-9950
IS - 4
ER -