Oxidative events in neuronal and glial cell-enriched fractions of rat cerebral cortex

Carla Café, Carla Torri, Laura Bertorelli, Fulvio Tartara, Flavio Tancioni, Paolo Gaetani, Riccardo Rodriguez Y Baena, Fulvio Marzatico

Research output: Contribution to journalArticle


The aim of this work was to investigate how neurons and glial cells separated from rat brain cortex respond to "in vitro" oxidative stress induced by incubation of the cellular fractions in the presence of prooxidant mixtures; in addition, the endogenous enzymatic antioxidant capacity of the purified fractions was investigated. Neuronal and glial cell-enriched fractions were obtained from rat cerebral cortex following passages of the tissue through meshes and centrifugations. The following parameters were evaluated: antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), and glucose-6-phosphate dehydrogenase (G6PDH); lipid peroxidation products (TBARS) prior to (basal) and after (iron-stimulated) incubation with a mixture of iron and ascorbic acid; intracellular production of reactive oxygen species (ROS) using a fluorescent probe, dichlorofluorescin-diacetate, in basal, iron-stimulated, and menadione stimulated conditions. SOD and GSHPx activities showed no significant changes between neurons and glia, whereas CAT and G6PDH activities were found to be significantly lower in glia than in neurons. TBARS levels were significantly lower in the glial fraction than in neurons, both in basal and iron-stimulated conditions. ROS production showed no differences between neurons and glia in both basal and menadione-stimulated conditions. Iron-stimulation produced a marked increase in ROS production, limited to the neuronal fraction, with the glial values being similar to the basal ones. Our conclusion is that glia and neurons isolated from rat cerebral cortex show a similar pattern of the most important antioxidant enzymes and of their basal ROS production, whereas glia is more resistant in "oxidative stress" conditions.

Original languageEnglish
Pages (from-to)853-857
Number of pages5
JournalFree Radical Biology and Medicine
Issue number6
Publication statusPublished - 1995


  • Antioxidant enzymes
  • Dichlorofluorescindiacetate
  • Glial enriched fractions
  • Lipid peroxidation
  • Neuronal enriched fractions
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)
  • Clinical Biochemistry
  • Medicine(all)
  • Toxicology

Fingerprint Dive into the research topics of 'Oxidative events in neuronal and glial cell-enriched fractions of rat cerebral cortex'. Together they form a unique fingerprint.

  • Cite this

    Café, C., Torri, C., Bertorelli, L., Tartara, F., Tancioni, F., Gaetani, P., Rodriguez Y Baena, R., & Marzatico, F. (1995). Oxidative events in neuronal and glial cell-enriched fractions of rat cerebral cortex. Free Radical Biology and Medicine, 19(6), 853-857. https://doi.org/10.1016/0891-5849(95)00086-D