Oxidative metabolism of human peripheral blood eosinophils and neutrophils: H2O2 production after stimulation with phorbol myristate acetate and immune complexes

G. A. Rossi, O. Sacco, F. Vassallo, S. Lantero, A. Morelli, U. Benatti, G. Damiani

Research output: Contribution to journalArticlepeer-review

Abstract

Eosinophils like neutrophils posses an oxidative metabolism which is activated by phagocytosis and cell membrane activation. Since eosinophils may take part in inflammatory reactions by releasing oxygen species and have membrane receptors for immunoglobulins, immune complexes may activate their respiratory burst. We compared the ability of phorbol myristate acetate (PMA) and immunoglobulin G (IgG) and immunoglobulin E (IgE) immune complexes to stimulate hydroge peroxide (H2O2) release from circulating eosinophils and neutrophils. PMA was able to stimulate a significant respiratory burst in both cell types, PMA-induced H2O2 production being higher in eosinophils than in neutrophils (p <0.05). Similar results were obtained when the cells were stimulated by IgG (p <0.05). In contrast, aggregated human myeloma IgE induced a detectable H2O2 release from peripheral blood eosinophils. We conclude that H2O2 release from eosinophils may play a role in parenchymal injury associated with hypereosinophilic disorders and the presence of immune complexes at sites of disease activity.

Original languageEnglish
JournalEuropean Respiratory Journal
Volume2
Issue numberSUPPL. 6
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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