Diabetes mellitus is characterized by an increased incidence of vascular thrombosis. It has been recently suggested that oxidative stress may play an important role in the pathogenesis of diabetic vascular complications. We evaluated glycemia, HbA1c, malondialdehyde (MDA, a specific free radical-dependent damage marker), prothrombin fragments 1 + 2 (F1 + 2, an index of in vivo thrombin formation) in 22 diabetic patients, on insulin therapy, and in 21 matched healthy controls. In the diabetic patients F1 + 2 (0.89 ± 0.38 vs 0.64 ± 0.13 nmol/L, p <0.009; M ± SD) and MDA (0.69 ± 0.14 vs 0.54 ± 0.8 μmol/L, p <0.003) were increased. Moreover, a good correlation between MDA and F1 + 2 (r = 0.48, p <0.02) and Hba1c (r = 0.44, p <0.05) was found. In 9 diabetics three different experiments were performed: OGTT, Glutathione (GSH) i.v. administration, OGTT plus GSH i.v. administration. Samples were drawn every 30 min, from 0 to 180 min. During OGTT, F1 + 2 plasma levels significantly increased from 30 to 120 min (p <0.01). GSH administration during OGTT normalized this phenomenon. GSH administered alone significantly decreased F1 + 2 plasma levels (p <0,01). These data show and increased thrombophilia in diabetes mellitus, and that oxidative stress, which seems to be linked to hyperglycemia, may condition this phenomenon.
|Translated title of the contribution||Oxidative stress and trombophilia in diabetes mellitus|
|Number of pages||4|
|Journal||Giornale Italiano di Chimica Clinica|
|Publication status||Published - 1994|
ASJC Scopus subject areas
- Clinical Biochemistry