Oxidative stress in patients with chronic obstructive pulmonary disease: Validation of a new photometric test (exhalation test) for the measurement of hydrogen peroxide in exhaled breath condensate

Gaetano Melillo, Eugenio Luigi Iorio, Franca Giuliano, Giovanni Balzano, Enrico Maria Melillo

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction. Increased oxidative stress in smokers and patients with chronic obstructive pulmonary disease (COPD) has been reported in several studies. Different methods have shown that various markers of oxidative stress may be found in serum, urine and exhaled breath. Aim. Aim of this study was the evaluation of systemic oxidative stress in serum and local oxidative stress in exhaled breath condensate (EBC) in patients (n = 54) with COPD and healthy control subjects (n = 12), by means of a new photometric method. Methods. We used two new photometric methods: the d-ROMs test to measure serum hydroperoxides and the exhalation test to measure hydrogen peroxide (H2O2) in EBC. Results. Mean level of serum hydroperoxides was 157 ± 81 CARR U in healthy non smoking subjects and 286 ± 60 CARR U in COPD patients. Mean H2O2 level in EBC was 0.33 ± 0.54 μM in healthy non smoking subjects and 2.84 ± 3.5 μM in CODP patients. Systemic oxidative stress, evaluated through the d-ROMs test, increased in 19 out of 54 studied patients (35.1%). Local oxidative stress, evaluated through the exhalation test, increased in 37 out of 54 patients (68%). Correlation was observed neither between expired H2O2 levels and cigarette smoking status (non smokers: 2.32 ± 1.48 μM H2O2; former smokers: 2.90 ± 3.71 μM H2O2; current smokers: 3.22 ± 4.34 μM H2O2), nor between various degrees of airway obstruction severity (mild obstruction: 3.63 ± 1.83 μM H2O2; moderate obstruction: 2.23 ± 2.28 μM H2O2; severe obstruction: 3.25 ± 4.44 μM H2O2). No correlation was then observed between expired H2O2 levels and arterial oxygen levels (mild hypoxemia: 4.59 ± 6.28 μM H2O2; moderate hypoxemia: 3.56 ± 2.4 pM H2O2). Conclusions. This study points out better sensitivity of the exhalation test compared to serum test (d-ROMs test) in evaluating oxidative stress levels in COPD patients. Moreover, exhalation test proved to be a simple, easy, non invasive and time saving photometric test for the measurement of oxidative stress in COPD patients: therefore, it could be also usefully adopted in other chronic inflammatory lung diseases.

Original languageEnglish
Pages (from-to)98-104
Number of pages7
JournalRassegna di Patologia dell'Apparato Respiratorio
Volume22
Issue number2
Publication statusPublished - Apr 2007

Keywords

  • COPD
  • d-ROMs test
  • Exhalation test
  • Exhaled breath condensate
  • Hydrogen peroxide
  • Oxidative stress

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Pulmonary and Respiratory Medicine

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