Oxidative stress, sister chromatid exchanges and apoptosis in the pathogenesis of lymphocytopenia in ESRD patients

Francesco Pernice, Fulvio Floccari, Lorena Nostro, Chiara Caccamo, Nadia Belghity, Stefania Mantuano, Adolfo Romeo, Antonio Barillà, Carmela Aloisi, Antonella Ruello, Nicola Frisina, Michele Buemi

Research output: Contribution to journalArticlepeer-review


Background: In end-stage renal disease (ESRD) patients on hemodialysis (HD) there may be a link between oxidative stress, genomic damage and the tendency of peripheral lymphocytes to die by apoptosis. Our aim was to verify this hypothesis, and to ascertain whether the link, if present, could explain lymphopenia in uremic patients. Methods: The series investigated comprised 55 participants: 30 HD patients on regular maintenance acetate-free biofiltration (AFB) and 25 age-matched healthy volunteers. One blood sample was drawn from the cubital vein of each participant. In HD patients, samples were drawn 3 times: predialytic, postdialytic and interdialytic (24 hours after the end of the session). Thiobarbituric acid reactants (TBARs), sister chromatid exchange (SCE) rate, high frequency cells (HFCs), total circulating lymphocytes and the percentage of circulating apoptotic lymphocytes were assayed in all samples. A statistical analysis of the findings was made using multiple and linear regression. Results: In AFB patients, TBAR levels appeared higher than in controls, even at baseline (2.15 ± 0.5 μmol/L vs. 1.20 ± 0.4 μmol/L; p

Original languageEnglish
Pages (from-to)613-620
Number of pages8
JournalJournal of Nephrology
Issue number5
Publication statusPublished - Sep 2006


  • AFB
  • Apoptosis
  • Lymphocytopenia
  • Oxidative stress
  • Sister chromatid exchange

ASJC Scopus subject areas

  • Nephrology


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