Oxidative stress, sister chromatid exchanges and apoptosis in the pathogenesis of lymphocytopenia in ESRD patients

Francesco Pernice, Fulvio Floccari, Lorena Nostro, Chiara Caccamo, Nadia Belghity, Stefania Mantuano, Adolfo Romeo, Antonio Barillà, Carmela Aloisi, Antonella Ruello, Nicola Frisina, Michele Buemi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: In end-stage renal disease (ESRD) patients on hemodialysis (HD) there may be a link between oxidative stress, genomic damage and the tendency of peripheral lymphocytes to die by apoptosis. Our aim was to verify this hypothesis, and to ascertain whether the link, if present, could explain lymphopenia in uremic patients. Methods: The series investigated comprised 55 participants: 30 HD patients on regular maintenance acetate-free biofiltration (AFB) and 25 age-matched healthy volunteers. One blood sample was drawn from the cubital vein of each participant. In HD patients, samples were drawn 3 times: predialytic, postdialytic and interdialytic (24 hours after the end of the session). Thiobarbituric acid reactants (TBARs), sister chromatid exchange (SCE) rate, high frequency cells (HFCs), total circulating lymphocytes and the percentage of circulating apoptotic lymphocytes were assayed in all samples. A statistical analysis of the findings was made using multiple and linear regression. Results: In AFB patients, TBAR levels appeared higher than in controls, even at baseline (2.15 ± 0.5 μmol/L vs. 1.20 ± 0.4 μmol/L; p

Original languageEnglish
Pages (from-to)613-620
Number of pages8
JournalJournal of Nephrology
Volume19
Issue number5
Publication statusPublished - Sep 2006

Fingerprint

Sister Chromatid Exchange
Lymphopenia
Chronic Kidney Failure
Oxidative Stress
Apoptosis
Hemodiafiltration
Renal Dialysis
Lymphocytes
Veins
Linear Models
Healthy Volunteers
Maintenance

Keywords

  • AFB
  • Apoptosis
  • Lymphocytopenia
  • Oxidative stress
  • Sister chromatid exchange

ASJC Scopus subject areas

  • Nephrology

Cite this

Pernice, F., Floccari, F., Nostro, L., Caccamo, C., Belghity, N., Mantuano, S., ... Buemi, M. (2006). Oxidative stress, sister chromatid exchanges and apoptosis in the pathogenesis of lymphocytopenia in ESRD patients. Journal of Nephrology, 19(5), 613-620.

Oxidative stress, sister chromatid exchanges and apoptosis in the pathogenesis of lymphocytopenia in ESRD patients. / Pernice, Francesco; Floccari, Fulvio; Nostro, Lorena; Caccamo, Chiara; Belghity, Nadia; Mantuano, Stefania; Romeo, Adolfo; Barillà, Antonio; Aloisi, Carmela; Ruello, Antonella; Frisina, Nicola; Buemi, Michele.

In: Journal of Nephrology, Vol. 19, No. 5, 09.2006, p. 613-620.

Research output: Contribution to journalArticle

Pernice, F, Floccari, F, Nostro, L, Caccamo, C, Belghity, N, Mantuano, S, Romeo, A, Barillà, A, Aloisi, C, Ruello, A, Frisina, N & Buemi, M 2006, 'Oxidative stress, sister chromatid exchanges and apoptosis in the pathogenesis of lymphocytopenia in ESRD patients', Journal of Nephrology, vol. 19, no. 5, pp. 613-620.
Pernice F, Floccari F, Nostro L, Caccamo C, Belghity N, Mantuano S et al. Oxidative stress, sister chromatid exchanges and apoptosis in the pathogenesis of lymphocytopenia in ESRD patients. Journal of Nephrology. 2006 Sep;19(5):613-620.
Pernice, Francesco ; Floccari, Fulvio ; Nostro, Lorena ; Caccamo, Chiara ; Belghity, Nadia ; Mantuano, Stefania ; Romeo, Adolfo ; Barillà, Antonio ; Aloisi, Carmela ; Ruello, Antonella ; Frisina, Nicola ; Buemi, Michele. / Oxidative stress, sister chromatid exchanges and apoptosis in the pathogenesis of lymphocytopenia in ESRD patients. In: Journal of Nephrology. 2006 ; Vol. 19, No. 5. pp. 613-620.
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AU - Romeo, Adolfo

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