Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells

Elisa Callegari; Giuseppe D. Norata; Hiroyasu Inoue; Alberico L. Catapano

Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells

Elisa Callegari, Giuseppe D. Norata, Hiroyasu Inoue, Alberico L. Catapano

IRCCS Multimedica

Research output: Contribution to journal › Article › peer-review

Abstract

Modified high density lipoprotein (HDL) has been suggested to modulate endothelial expression of proinflammatory genes. Since oxidised HDL (Ox-HDL) has been found in atheromatous plaques and receptors for modified HDL are present on endothelial cells, we investigated the effect of Ox-HDL₃ on the expression of Cox-1 or Cox-2. Ox-HDL₃, increased Cox-2 mRNA and protein expression in endothelial cells while no effect on Cox-1 expression was observed. The intracellular pathways involved in this effect were investigated. The incubation with specific inhibitors of intracellular kinases showed that PI3K is mainly involved in the Ox-HDL₃-dependent Cox-2 induction. Transient transfection experiments suggested that the NF-IL6 response element in the proximal promoter (-327 to 59) is involved in Ox-HDL₃-mediated Cox-2 expression. These data suggest that Ox-HDL induce Cox-2 expression in endothelial cells through a PI3K/NF-IL6-dependent pathway.

Original language	English
Pages (from-to)	209-213
Number of pages	5
Journal	International Journal of Molecular Medicine
Volume	18
Issue number	1
Publication status	Published - Jul 2006

Keywords

Cox-2
Endothelial cells
High density lipoprotein

ASJC Scopus subject areas

Genetics

Cite this

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title = "Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells",

abstract = "Modified high density lipoprotein (HDL) has been suggested to modulate endothelial expression of proinflammatory genes. Since oxidised HDL (Ox-HDL) has been found in atheromatous plaques and receptors for modified HDL are present on endothelial cells, we investigated the effect of Ox-HDL3 on the expression of Cox-1 or Cox-2. Ox-HDL3, increased Cox-2 mRNA and protein expression in endothelial cells while no effect on Cox-1 expression was observed. The intracellular pathways involved in this effect were investigated. The incubation with specific inhibitors of intracellular kinases showed that PI3K is mainly involved in the Ox-HDL3-dependent Cox-2 induction. Transient transfection experiments suggested that the NF-IL6 response element in the proximal promoter (-327 to 59) is involved in Ox-HDL3-mediated Cox-2 expression. These data suggest that Ox-HDL induce Cox-2 expression in endothelial cells through a PI3K/NF-IL6-dependent pathway.",

keywords = "Cox-2, Endothelial cells, High density lipoprotein",

author = "Elisa Callegari and Norata, {Giuseppe D.} and Hiroyasu Inoue and Catapano, {Alberico L.}",

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T1 - Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells

AU - Callegari, Elisa

AU - Norata, Giuseppe D.

AU - Inoue, Hiroyasu

AU - Catapano, Alberico L.

PY - 2006/7

Y1 - 2006/7

N2 - Modified high density lipoprotein (HDL) has been suggested to modulate endothelial expression of proinflammatory genes. Since oxidised HDL (Ox-HDL) has been found in atheromatous plaques and receptors for modified HDL are present on endothelial cells, we investigated the effect of Ox-HDL3 on the expression of Cox-1 or Cox-2. Ox-HDL3, increased Cox-2 mRNA and protein expression in endothelial cells while no effect on Cox-1 expression was observed. The intracellular pathways involved in this effect were investigated. The incubation with specific inhibitors of intracellular kinases showed that PI3K is mainly involved in the Ox-HDL3-dependent Cox-2 induction. Transient transfection experiments suggested that the NF-IL6 response element in the proximal promoter (-327 to 59) is involved in Ox-HDL3-mediated Cox-2 expression. These data suggest that Ox-HDL induce Cox-2 expression in endothelial cells through a PI3K/NF-IL6-dependent pathway.

AB - Modified high density lipoprotein (HDL) has been suggested to modulate endothelial expression of proinflammatory genes. Since oxidised HDL (Ox-HDL) has been found in atheromatous plaques and receptors for modified HDL are present on endothelial cells, we investigated the effect of Ox-HDL3 on the expression of Cox-1 or Cox-2. Ox-HDL3, increased Cox-2 mRNA and protein expression in endothelial cells while no effect on Cox-1 expression was observed. The intracellular pathways involved in this effect were investigated. The incubation with specific inhibitors of intracellular kinases showed that PI3K is mainly involved in the Ox-HDL3-dependent Cox-2 induction. Transient transfection experiments suggested that the NF-IL6 response element in the proximal promoter (-327 to 59) is involved in Ox-HDL3-mediated Cox-2 expression. These data suggest that Ox-HDL induce Cox-2 expression in endothelial cells through a PI3K/NF-IL6-dependent pathway.

KW - Cox-2

KW - Endothelial cells

KW - High density lipoprotein

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Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells

Abstract

Keywords

ASJC Scopus subject areas

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