Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells

Elisa Callegari, Giuseppe D. Norata, Hiroyasu Inoue, Alberico L. Catapano

Research output: Contribution to journalArticle

Abstract

Modified high density lipoprotein (HDL) has been suggested to modulate endothelial expression of proinflammatory genes. Since oxidised HDL (Ox-HDL) has been found in atheromatous plaques and receptors for modified HDL are present on endothelial cells, we investigated the effect of Ox-HDL3 on the expression of Cox-1 or Cox-2. Ox-HDL3, increased Cox-2 mRNA and protein expression in endothelial cells while no effect on Cox-1 expression was observed. The intracellular pathways involved in this effect were investigated. The incubation with specific inhibitors of intracellular kinases showed that PI3K is mainly involved in the Ox-HDL3-dependent Cox-2 induction. Transient transfection experiments suggested that the NF-IL6 response element in the proximal promoter (-327 to 59) is involved in Ox-HDL3-mediated Cox-2 expression. These data suggest that Ox-HDL induce Cox-2 expression in endothelial cells through a PI3K/NF-IL6-dependent pathway.

Original languageEnglish
Pages (from-to)209-213
Number of pages5
JournalInternational Journal of Molecular Medicine
Volume18
Issue number1
Publication statusPublished - Jul 2006

Keywords

  • Cox-2
  • Endothelial cells
  • High density lipoprotein

ASJC Scopus subject areas

  • Genetics

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