Abstract
Modified high density lipoprotein (HDL) has been suggested to modulate endothelial expression of proinflammatory genes. Since oxidised HDL (Ox-HDL) has been found in atheromatous plaques and receptors for modified HDL are present on endothelial cells, we investigated the effect of Ox-HDL3 on the expression of Cox-1 or Cox-2. Ox-HDL3, increased Cox-2 mRNA and protein expression in endothelial cells while no effect on Cox-1 expression was observed. The intracellular pathways involved in this effect were investigated. The incubation with specific inhibitors of intracellular kinases showed that PI3K is mainly involved in the Ox-HDL3-dependent Cox-2 induction. Transient transfection experiments suggested that the NF-IL6 response element in the proximal promoter (-327 to 59) is involved in Ox-HDL3-mediated Cox-2 expression. These data suggest that Ox-HDL induce Cox-2 expression in endothelial cells through a PI3K/NF-IL6-dependent pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 209-213 |
| Number of pages | 5 |
| Journal | International Journal of Molecular Medicine |
| Volume | 18 |
| Issue number | 1 |
| Publication status | Published - Jul 2006 |
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Keywords
- Cox-2
- Endothelial cells
- High density lipoprotein
ASJC Scopus subject areas
- Genetics
Cite this
Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells. / Callegari, Elisa; Norata, Giuseppe D.; Inoue, Hiroyasu; Catapano, Alberico L.
In: International Journal of Molecular Medicine, Vol. 18, No. 1, 07.2006, p. 209-213.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells
AU - Callegari, Elisa
AU - Norata, Giuseppe D.
AU - Inoue, Hiroyasu
AU - Catapano, Alberico L.
PY - 2006/7
Y1 - 2006/7
N2 - Modified high density lipoprotein (HDL) has been suggested to modulate endothelial expression of proinflammatory genes. Since oxidised HDL (Ox-HDL) has been found in atheromatous plaques and receptors for modified HDL are present on endothelial cells, we investigated the effect of Ox-HDL3 on the expression of Cox-1 or Cox-2. Ox-HDL3, increased Cox-2 mRNA and protein expression in endothelial cells while no effect on Cox-1 expression was observed. The intracellular pathways involved in this effect were investigated. The incubation with specific inhibitors of intracellular kinases showed that PI3K is mainly involved in the Ox-HDL3-dependent Cox-2 induction. Transient transfection experiments suggested that the NF-IL6 response element in the proximal promoter (-327 to 59) is involved in Ox-HDL3-mediated Cox-2 expression. These data suggest that Ox-HDL induce Cox-2 expression in endothelial cells through a PI3K/NF-IL6-dependent pathway.
AB - Modified high density lipoprotein (HDL) has been suggested to modulate endothelial expression of proinflammatory genes. Since oxidised HDL (Ox-HDL) has been found in atheromatous plaques and receptors for modified HDL are present on endothelial cells, we investigated the effect of Ox-HDL3 on the expression of Cox-1 or Cox-2. Ox-HDL3, increased Cox-2 mRNA and protein expression in endothelial cells while no effect on Cox-1 expression was observed. The intracellular pathways involved in this effect were investigated. The incubation with specific inhibitors of intracellular kinases showed that PI3K is mainly involved in the Ox-HDL3-dependent Cox-2 induction. Transient transfection experiments suggested that the NF-IL6 response element in the proximal promoter (-327 to 59) is involved in Ox-HDL3-mediated Cox-2 expression. These data suggest that Ox-HDL induce Cox-2 expression in endothelial cells through a PI3K/NF-IL6-dependent pathway.
KW - Cox-2
KW - Endothelial cells
KW - High density lipoprotein
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UR - http://www.scopus.com/inward/citedby.url?scp=33748195217&partnerID=8YFLogxK
M3 - Article
C2 - 16786175
AN - SCOPUS:33748195217
VL - 18
SP - 209
EP - 213
JO - International Journal of Molecular Medicine
JF - International Journal of Molecular Medicine
SN - 1107-3756
IS - 1
ER -